doi:

DOI: 10.3724/SP.J.1008.2010.00822

Academic Journal of Second Military Medical University (第二军医大学学报) 2010/30:8 PP.822-825

Prediction of B cell epitopes of tumor-associated protein EIF4G1 subtypes


Abstract:
Objective To predict the B cell epitopes of tumor-associated protein EIF4G1 subtypes.Methods The sequences of all the protein subtypes of EIF4G1 were retrieved from NCBI protein database.Based on single parameter evaluation,including hydrophilicity,flexility,antigenicity,the B cell epitopes of the EIF4G1 protein subtypes were predicted using NPS@structure software and ABCpred software.Results EIF4G1 protein had five subtypes.The variation of the five different EIF4G1 subtypes was limited within a 300aa region.We identified eight epitopes locating in or near 14-19,21-27,52-61,106-112,113-139,183-189,201-216, and 217-224, which can be used to identify specific B cell epitopes of different protein subtypes. Conclusion B cell epitopes of EIF4G1 protein subtypes do exist, and they may be used for the protein subtypes evaluation and early diagnosis of tumor patients using artificially-produced matched peptides.

Key words:eukaryotic initiation factor-4G,protein isofroms,B lymphocytes epitopes,neoplasms

ReleaseDate:2014-07-21 15:25:16

Funds:National Natural Science Foundation of China (30872562) and Innovation Program of Shanghai Municipal Education Commission(08ZZ39).



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