Academic Journal of Second Military Medical University (第二军医大学学报) 2015/36:12 PP.1295-1299
Objective To explore the effect of vitamin E (VE) against the neurotoxicity of benzo[a]pryene (B[a]P) in male SD rats. Methods A total of 60 male SD rats were randomly assigned to one of the following six groups (n=10/group): the blank control group, vehicle control group, B[a]P group(5 mg/kg), low dose of VE (10 mg/kg) + B[a]P (5 mg/kg) group, medium dose of VE (50 mg/kg) + B[a]P (5 mg/kg) group and high dose of VE (100 mg/kg) + B[a]P (5 mg/kg) group. The rats were gavaged with the corresponding dose of B[a]P once a day for 30 days. Morris water maze was used to evaluate the learning and memory performance of rats. Morphological changes in hippocampus were observed. The activities of superoxide dismutase(SOD), glutathione peroxidase(GSH-Px) and γ-glutamylcysteine synthetase(γ-GCS), the contents of glutathione(GSH), oxidized glutathione (GSSG) and malonaldehyde (MDA) in the hippocampus tissue were all examined. Results The results of the behavior tests showed that rats treated with B[a]P exhibited a significantly increased escape latency and a significantly decreased cross-platform times and the residence time on the platform compared with the blank control group and vehicle group (P<0.01). VE treatment could obviously improve the changes of the above index (P<0.01). Besides, the morphological changes in hippocampus suggested that VE could protect against the injury induced by B[a]P. The activities of SOD, GSH-Px and γ-GCS and the contents of GSH, GSSG in rat hippocampus of B[a]P group were significantly decreased, while the content of MDA was significantly increased compared with the blank control group and vehicle control group (P<0.01). Moreover, the above indices were obviously improved with the increase of VE dosage in VE groups. Conclusion VE can play an protective role in B[a]P-induced neurotoxicity.
 李军生, 邹义英.维生素E作用机制研究新进展[J].中国医院药学杂志, 2005, 25: 556-558.
 陈承志, 汤 艳, 蒋学君, 涂白杰.苯并[a]芘对大鼠学习记忆及海马神经元影响[J].中国公共卫生, 2011, 27: 608-610.
 Zhang H, Nie J, Li X, Niu Q. Association of aryl hydrocarbon receptor gene polymorphism with the neurobehavioral function and autonomic nervous system function changes induced by benzo[a]pyrene exposure in coke oven workers [J]. J Occupa Environmental Med, 2013, 55: 265-271.
 汤 艳, 陈承志, 郭庆喜, 戚友斌, 涂白杰. 14C-苯并(a)芘在大鼠海马中的分布及对海马神经元的损害[J].环境与职业医学, 2011, 28: 141-143.
 Qiu C Y, Cheng S Q, Xia Y Y, Peng B, Tang Y, Tu B J. Effects of subchronic benzo(a)pyrene exposure on neurotransmitter receptor gene expression in the rat hippocampus related with spatial learning and memory change [J]. Toxicology, 2011, 289(2-3):83-90.
 李金艳, 常珊珊, 袁晋峰, 王 鑫, 蒋 勇, 郑金平.亚慢性染毒B[a]P与大鼠海马细胞凋亡及学习记忆损伤的关系[J].环境与职业医学, 2012, 29: 118-121.
 初巍巍, 霍 阳, 陈 悦, 宋 涛, 赵跃萍, 王 莉. 苯并芘氧化损伤鼠肺泡细胞及维生素E对其保护作用的机制研究[J]. 解放军医药杂志, 2013, 25: 66-68.
 沈梅红, 李 成, 李忠仁. 电针对脑缺血再灌注模型大鼠的GSH含量、GSH-Px及GR活性的影响[J]. 南京中医药大学学报, 2011, 27: 137-139.
 房 群, 马爱国, 孙永叶. 大剂量维生素E补充对大鼠抗氧化能力和红细胞膜流动性无明显改善作用[J]. 医学信息, 2011, 24: 4-5.
 李 斐, 赵 瑛. 津力达颗粒对糖尿病大鼠海马组织的保护作用[J]. 第二军医大学学报, 2013, 34: 137-141. Li F, Zhao Y. Protective effect of Jinlida granules on hippocampus of diabetic rats[J]. Acad J Sec Mil Med Univ, 2013, 34: 137-141.
 邰 贺, 王 丹, 梁 艳, 戴 红, 陈亚琼. 苯并芘氧化损伤胎盘组织作用机制及维生素E防护作用探讨[J]. 临床误诊误治, 2013, 26: 94-96.
 初巍巍, 霍 阳, 王 莉, 陈 悦, 宋 涛, 赵跃萍.黄芪注射液对苯并芘损伤的肺泡组织氧化相关物质的影响[J].临床误诊误治, 2013, 26: 90-92.
 李 明, 严律南. 益生注射液抗大鼠移植心慢性失功加快模型的实验研究[J]. 重庆医科大学学报, 2011, 36: 294-297.
 Liang X, Tang Y, Duan L, Cheng S Q, Luo L, Cao X, et al. Adverse effect of sub-chronic exposure to benzo(a)pyrene and protective effect of butylated hydroxyanisole on learning and memory ability in male Sprague-Dawley rat [J]. J Toxicol Sci, 2014, 39: 739-748.