doi:

DOI: 10.3724/SP.J.1008.2008.01069

Academic Journal of Second Military Medical University (第二军医大学学报) 2008/28:9 PP.1069-1073

Influence of glucosidorum tripterygii tororum on expression of interleukin-23, interleukin-17 and interleukin-12 in colonic tissues of mice with colitis


Abstract:
Objective:To study the influence of glucosidorum tripterygii tororum (GTT) and Mesalazine on the expression of IL-23, IL-17 and IL-12 in the colonic tissues of mice with rinitrobenzene sulphonic acid (TNBS)-induced acute colitis, and to study the possible mechanism.Methods: The C57BL/6 mice were divided into 4 groups, namely, a control group, a model group, a Mesalazine group and a GTT group. Colitis was induced by TNBS in the last 3 groups. The mice in the model group received no additional treatment; those in the GTT group received GTT daily by oral gavage 4 days before exposure to TNBS till the end of the experiment, and those in the Mesalazine group received Mesalazine enema solution daily 4 days before exposure till the end of the experiment. All the mice were sacrificed at 48 h after enema with TNBS. The macroscopic and histological scores of colon damage and myeloperoxidase (MPO) activity in colonic tissue were evaluated in every group. IL-23p19 and IL-17 contents in colonic tissues were measured by ELISA; the expression of IL-23p19, IL-17 and IL-12p35 mRNA in colonic tissues were examined by real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (RT-FQ-PCR) with SYBR Green Ⅰ.Results: Compared with the model group, GTT group and Mesalazine group had significantly lower macroscopic and histological scores and MPO activity (P<0.05). Expression of IL-23p19, IL-17 and IL-12p35 mRNA in the colonic tissues of the model group was significantly higher than that of the other 3 groups(P<0.05); the expression of IL-23p19, IL-17 protein was significant higher in the model group than that in the other 3 groups (P<0.05), with no significant difference found between the later 3 groups.Conclusion: GTT, like Mesalazine, can effectively inhibit inflammation in mice with TNBS-induced acute colitis through non-selectively inhibiting the expression of IL-23p19, IL-17 and IL-12p35.

Key words:colitis,interleukin-23,interleukin-17,interleukin-12,glucosidorum tripterygii tororum,mesalazine,trinitrobenzenesulfonic acid

ReleaseDate:2014-07-21 14:44:53



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