DOI: 10.3724/SP.J.1008.2010.00012

Academic Journal of Second Military Medical University (第二军医大学学报) 2010/30:1 PP.12-17

Relationship of GSTP1,RASSF1A polymorphisms and environmental agent with susceptibility to prostate cancer:a case-control study

Objective To investigate relationship of GSTP1,RASSF1A polymorphisms and environmental agent with susceptibility to prostate cancer (Pca).Methods The GSTP1 and RASSF1A genotypes were determined by TaqMan/MGB Probe Technology in 103 patients with Pca and 103 normal controls. Multivariate logistic regression model was used to assess the association of smoking,alcohol drinking,tea drinking,weekly pork and beef consumption,and the genetic polymorphisms with the susceptibility to Pca,while taking into consideration of the environmental agent. Results The frequencies of the GSTP1 AA,AG and GG genotypes were 66.02%, 22.33%, and 11.65% in patients with Pca and 67.96%, 29.13% and 2.91% in controls,respectively,with significant difference found between the two groups (χ2=6.35,P=0.04). The frequencies of RASSF1A CC,CA and AA genotypes were 88.34%,5.83%, and 5.83% in patients with Pca,and 85.44%,12.62%, and 1.94% in the controls, respectively,with no significant difference found between the two groups (χ2=4.63,P=0.10).Multivariate analysis showed a decreased risk in those who had a tea drinking history (OR=0.40,95%CI,0.19-0.82) and an increased risk in those who had a smoking history (OR=3.02,95%CI,1.44-6.32).Conclusion Our results indicate that GSTP1,RASSF1A polymorphisms are not associated with Pca susceptibility in Chinese Han nationality. Smoking is the risk factor of Pca,and tea drinking is a protective factor against Pca.

Key words:prostatic neoplasms,case-control studies,GSTP1,RASSF1A,genetic polymorphism

ReleaseDate:2014-07-21 15:07:27

Funds:Supported by National Natural Science Foundation of China(30671793).

[1] 孙颖浩.我国前列腺癌研究现状[J].中华泌尿外科杂志,2004,25:77-79.

[2] Mannervik B,Awasthi Y C,Board P G,Hayes J D,Di Ilio C,Ketterer B,et al. Nomenclature for human glutathione transferases[J]. Biochem J,1992,282(Pt1):305-306.

[3] Borad P G,Webb G C,Coggan M.Isolation of ac DNA clone and localization of the human glutathione S-transferase 3 genes to chromosome bands 11q13 and 12q13-14[J].Ann Hum Genet,1989,53(pt3):205-213.

[4] Lee S A,Fowke J H,Lu W,Ye C,Zheng Y,Cai Q,et al.Cruciferous vegetables,the GSTP1 Ile105Val genetic polymorphism,and breast cancer risk[J]. Am J Clin Nutr,2008,87:753-760.

[5] Rossini A,Rapozo D C,Soares Lima S C,GuimarAes D P,Ferreira M A,Teixeira R,et al. Polymorphisms of GSTP1 and GSTT1,but not of CYP2A6,CYP2E1 or GSTM1,modify the risk for esophageal cancer in a western population[J]. Carcinogenesis,2007,28:2537-2542.

[6] Vlaykova T,Miteva L,Gulubova M,Stanilova S.Ile105Val GSTP1 polymorphism and susceptibility to colorectal carcinoma in Bulgarian population[J]. Int J Colorectal Dis,2007,22:1209-1215.

[7] Sun N,Sun X,Chen B,Cheng H,Feng J,Cheng L,et al. MRP2 and GSTP1 polymorphisms and chemotherapy response in advanced non-small cell lung cancer[J].Cancer Chemother Pharmacol,2010,65:437-446.

[8] Kote-Jarai Z,Easton D,Edwards S M,Jefferies S,Durocher F,Jackson R A,et al. Relationship between glutathione S-transferase M1,P1 and T1 polymorphisms and early onset prostate cancer[J].Pharmacogenetics,2001,11:325-330.

[9] Nakazato H,Suzuki K,Matsui H,Koike H,Okugi H,Ohtake N,et al. Association of genetic polymorphisms of glutathione-S-transferase genes (GSTM1,GSTT1 and GSTP1) with familial prostate cancer risk in a Japanese population[J]. Anticancer Res,2003,23(3C):2897-2902.

[10] Srivastava D S,Mandhani A,Mittal B,Mittal R D.Genetic polymorphism of glutathione S-transferase genes (GSTM1,GSTT1 and GSTP1) and susceptibility to prostate cancer in Northern India[J]. BJU Int,2005,95:170-173.

[11] Kidd L C,Woodson K,Taylor P R,Albanes D,Virtamo J,Tangrea J A.Polymorphisms in glutathione-S-transferase genes (GST-M1,GST-T1 and GST-P1) and susceptibility to prostate cancer among male smokers of the ATBC cancer prevention study[J]. Eur J Cancer Prev,2003,12:317-320.

[12] Nam R K,Zhang W W,Trachtenberg J,Jewett M A,Emami M,Vesprini D,et al. Comprehensive assessment of candidate genes and serological markers for the detection of prostate cancer[J].Cancer Epidemiol Biomarkers Prev,2003,12:1429-1437.

[13] Mittal R D,Mishra D K,Mandhani A. Evaluating polymorphic status of glutathione-S-transferase genes in blood and tissue samples of prostate cancer patients[J].Asian Pac J Cancer Prev,2006,7:444-446.

[14] Agathanggelou A,Cooper W N,Latif F.Role of the Ras-association domain family 1 tumor suppressor gene in human cancers[J].Cancer Res,2005,65:3497-3508.

[15] Schagdarsurengin U,Seidel C,Ulbrich E J,KOlbl H,Dittmer J,Dammann R.A polymorphism at codon 133 of the tumor suppressor RASSF1A is associated with tumorous alteration of the breast[J].Int J Oncol,2005,27:185-191.

[16] Gao B,Xie X J,Huang C,Shames D S,Chen T T,Lewis C M,et al.RASSF1A polymorphism A133S is associated with early onset breast cancer in BRCA1/2 mutation carriers[J]. Cancer Res,2008,68:22-25.

[17] Lichtenstein P,Holm N V,Verkasalo P K,Iliadou A,Kaprio J,Koskenvuo M,et al. Environmental and heritable factors in the causation of cancer:analyses of cohorts of twins from Sweden,Denmark,and Finland[J].N Engl J Med,2000,343:78-85.

[18] Payne S R,Serth J,Schostak M,Kamradt J,Strauss A,Thelen P,et al. DNA methylation biomarkers of prostate cancer: confirmation of candidates and evidence urine is the most sensitive body fluid for non-invasive detection[J]. Prostate,2009,69:1257-1269.

[19] 杜稳斌,侯建国,武 旗,常文军,翟羽佳,林丽萍,等.前列腺癌组织中谷胱甘肽S-转移酶P1基因启动子区域甲基化序列分析[J].第二军医大学学报,2008,29:762-767. Du W B,Hou J G,Wu Q,Chang W J,Zhai Y J,Lin L P,et al. Promoter region methylation analysis of glutathione S-transferase p1 gene in prostate carcinoma[J]. Acad J Sec Mil Med Univ,2008,29:762-767.

[20] Bryzgunova O E,Morozkin E S,Yarmoschuk S V,Vlassov V V,Laktionov P P.Methylation-specific sequencing of GSTP1 gene promoter in circulating/extracellular DNA from blood and urine of healthy donors and prostate cancer patients[J].Ann N Y Acad Sci,2008,1137:222-225.

[21] 翟 芸,周钢桥,董晓佳,张秀梅,贺凤英,汪海建,等.大规模发掘及分型SNP技术平台的建立[J].军事医学科学院院刊,2004,28:52-60.

[22] Mo Z,Gao Y,Cao Y,Gao F,Jian L.An updating meta-analysis of the GSTM1,GSTT1,and GSTP1 polymorphisms and prostate cancer: a HuGE review[J]. Prostate,2009,69:662-688.

[23] Tommasi S,Dammann R,Zhang Z,Wang Y,Liu L,Tsark W M,et al.Tumor susceptibility of Rassf1a knockout mice[J]. Cancer Res,2005,65:92-98.

[24] Vos M D,Ellis C A,Bell A,Birrer M J,Clark G J.Ras uses the novel tumor suppressor RASSF1 as an effector to mediate apoptosis[J].J Biol Chem,2000,275:35669-35672.

[25] Nock N L,Cicek M S,Li L,Liu X,Rybicki B A,Moreira A,et al. Polymorphisms in estrogen bioactivation,detoxification and oxidative DNA base excision repair genes and prostate cancer risk[J]. Carcinogenesis,2006,27:1842-1848.

[26] Saleem M,Adhami V M,Siddiqui I A,Mukhtar H. Tea beverage in chemoprevention of prostate cancer: a mini-review[J]. Nutr Cancer,2003,47:13-23.

[27] Kurahashi N,Sasazuki S,Iwasaki M,Inoue M,Tsugane S,JPHC Study Group.Green tea consumption and prostate cancer risk in Japanese men: a prospective study[J]. Am J Epidemiol,2008,167:71-77.

[28] Park O J,Surh Y J.Chemopreventive potential of epigallocatechin gallate and genistein: evidence from epidemiological and laboratory studies[J]. Toxicol Lett,2004,150:43-56.