doi:

DOI: 10.3724/SP.J.1006.2008.00777

Acta Agronomica Sinica (作物学报) 2008/34:5 PP.777-782

Cloning of A S-Adenosylmethionine Synthetase Gene from Broomcorn Millet (Panicum miliaceum L.) and Its Expression during Drought and Re-watering


Abstract:
To explore more genes for the improvement of drought-tolerance and water use efficiency in plants, broomcorn millet (Panicum miliaceum L.) was used to investigate the differential gene expression during drought stress and re-watering after serious drought stress by constructing SSH-cDNA library in previous study. A full-length cDNA of S-adenosylmethionine synthetase (SAMS) gene was amplified from broomcorn millet using PCR. The PCR primers were designed based on an EST sequence highly similar to SAMS gene in the SSH-cDNA library and the sequences of SAMS genes from rice and barley. The full-length cDNA sequence of SAMS gene amplified from broomcorn millet was 1 293 bp in length and designated as PmSAMS, encoding 396 amino acids with the 3 typical domains of SAMS (N terminal domain, inter domain and C terminal domain). Multiple alignment analysis based on the amino acids encoded by some SAMS genes from broomcorn millet (P. miliaceum L.), potato (S. tuberosum), Arabidopsis (A. thaliana), sugar beet (B. vulgaris), barley (H. vulgare), litchi (L. chinensis), tomato (L. esculentum), and rice (O. sativa) indicated that SAMS was very conserved among different species of plants with 92–97% of sequence similarity of amino acids, and PmSAMS had the highest similarity with that of rice (97%). The expression patterns of PmSAMS during drought and re-watering after serious drought were investigated by means of semi-quantitative RT-PCR. The results showed that the expressions of PmSAMS were great at the beginning of drought treatment (soil relative moisture of 36%), then declined under serious drought (soil relative moisture of 24%) to less than the normal level, and increased at 2 h after re-watering, declined to the normal level at 6 h after re-watering. The expression patterns of PmSAMS during drought and re-watering after serious drought indicated that it was involved in the response of broomcorn millet to drought and re-watering and might be one of the key genes for drought tolerance and water use efficiency of broomcorn millet. The cloning of this gene provides the potential for its utilization in the improvement of drought-tolerance and water use efficiency in other plants.

Key words:Broomcorn millet,S-adenosylmethionine synthetase,Drought and re-watering,Express profile,Phylogenetic analysis

ReleaseDate:2014-07-21 14:20:02



Lin F Y, Hu Y G, Song G Q, Zhang H, Liu T M, He B R. Isolation and analysis of genes induced by rehydration after serious drought in broomcorn millet (Panicum miliaceum L.) by using SSH. Chin J Agric Biotechnol, 2006, 3: 237-242

Lin F-Y(林凡云), Hu Y-G(胡银岗), Song G-Q(宋国琦), He B-R(何蓓如). Gene expression profile analysis of broomcorn millet during rehydration after serious drought by means of SSH. J Northwest A F Univ (Nat Sci Edn)(西北农林科技大学学报·自然科学版), 2007, 35(3): 81-86 (in Chinese with English abstract)

Heby O, Persson L. Molecular genetics of polyamine synthesis in eukaryotic cells. Trends Biochem Sci, 1990, 15: 153-158

Tabor C W, Tabor H. Methionine adenosyltransferase (Sadenosylmethionine synthetase) and S-adenosylmethionine decarboxylase. Adv Enzymol, 1984, 56: 251-282

Yang S F, Hoffman N E. Ethylene biosynthesis and its regulation in higher plants. Ann Rev Plant Physiol Plant Mol Biol, 1984, 35: 155-189

Espartero J, Pintor-Toro J A, Pardo J M. Differential accumulation of S-adenosylmethionine synthetase transcripts in response to salt stress. Plant Mol Biol, 1994, 25: 217-227

Izhaki A, Shoseyov O, Weiss D. A petunia cDNA encoding S-adenosylmethionine synthase. Plant Physiol, 1995, 108: 841-842

Yu T(余涛), Zhi L-F(支立峰), Peng L(彭论), Li Y-S(李阳生), Zhu Y-G(朱英国). Cloning and characterization of a novel S-adenosyl-L-methionine synthase gene in tobacco. Wuhan Bot Res (武汉植物学研究), 2004, 22 (4): 277-283 (in Chinese with English abstract)

Ma X L, Wang Z L, Qi Y C, Zhao Y X, Zhang H. Isolation of S-adenosylmethionine synthetase gene from Suaeda salsa and its differential expression under NaCl stress. Acta Bot Sin, 2003, 45: 1359-1365

Zhang J-G(张建国), Li X-H(李新华), Yuan Z-Y(袁中一). Research advances in gene and structure of S-adenodyl-L-methionine synthetase. Industr Microbiol (工业微生物), 2005, 35(3): 39-44 (in Chinese with English abstract)

Gupta P, Duplessis S, White H, Karnosky D F, Martin F, Podila G K. Gene expression patterns of trembling aspen trees following long-term exposure to interacting elevated CO2 and tropospheric O3. New Phytol, 2005, 167: 129-142

Vanden B N, Crampton B G, Hein I, Birch P R, Berger D K. High-throughput screening of suppression subtractive hybridization cDNA libraries using DNA microarray analysis. BioTechniques, 2004, 37: 818-824

Luo Y-X(罗赟星), Zhao F-K(赵辅昆), Luo G-M(罗贵民), Yuan Z-Y(袁中一). Research and development of S-Adenosyl-L-methionine. Industr Microbiol (工业微生物), 2006, 36(1): 54-59 (in Chinese with English abstract)

Yang J-C(杨建昌), Zhang Y-J(张亚洁), Zhang J-H(张建华), Wang Z-Q(王志琴), Zhu Q-S(朱庆森). Changes in contents of polyamines in the flag leaf and their relationship with drought-resistance of rice cultivars under water deficit stress. Acta Agron Sin (作物学报), 2004, 30(11): 1069-1075 (in Chinese with English abstract)

Chen K-M(陈坤明), Zhang C-L(张承烈). Polyamine contents in the spring wheat leaves and their relations to drought-resistance. Acta Phytophysiol Sin (植物生理学报), 2000, 26(5): 381-386 (in Chinese with English abstract)

Breusegem Van F, Dekeyser R, Gielen J, Montagu Van M, Caplan A. Characterization of a S-adenosylmethionine synthetase gene in rice. Plant Physiol, 1994, 105: 1463-1464

Peleman J, Boerjan W, Engker G, Seurinck J, Botterman J, Alliotte T, Montagu Van M, Inzé D. Strong cellular preference in the expression of a housekeeping gene of Arabidopsis thaliana encoding S-adenosylmethionine synthetase. Plant Cell, 1989, 1: 81-93

Schröder G, Eichel J, Breining S, Schröder J. Three differentially expressed S-adenosylmethionine synthetases from Catharanthus roseus: Molecular and functional characterization. Plant Mol Biol, 1997, 33: 211-222

Shen B, Li C, Tarczynski M C. High free-methionine and decreased lignin content result from a mutation in the Arabidopsis S-adenosyl-L-methionine synthetase 3 gene. Plant J, 2002, 29: 371-380

Gómez-Gómez L, Carrasco P. Differential expression of the S-Adenosyl-L-Methionine synthase genes during pea development. Plant Physiol, 1998, 117: 397-405

Oono Y, Seki M, Nanjo T, Narusaka M, Fujita M, Satoh R, Satou M, Sakurai T, Ishida J, Akiyama K, Iida K, Maruyama K, Satoh S, Yamaguchi-Shinozaki K, Shinozaki K. Monitoring expression profiles of Arabidopsis gene expression during rehydration process after dehydration using ca.7000 full-length cDNA microarray. Plant J, 2003, 34: 868-887

PDF