DOI: 10.3724/SP.J.1006.2008.00578

Acta Agronomica Sinica (作物学报) 2008/34:4 PP.578-586

Molecular Tagging and Source Analysis of QTL for Elite Fiber Quality in Upland Cotton

With the development and application of molecular biology technology in recent years, several genetic maps developed from different combinations have been constructed and lots of QTL controlling fiber quality or yield components detected, which makes the breeding procedure more effective, immediate and time-saving. In this paper, both F2 and F2:3 populations were constructed using two elite upland cotton (Gossypium hirsutum L.) strains (variety), 7235 and Yumian 1 as parents. Linkage analysis with SSR molecular markers revealed that 80 loci were mapped into 19 linkage groups that covered 1 147.8 cM genetic distance. A total of 36 QTLs were detected by composite interval mapping method, including 6 for fiber length, 8 for fiber strength, 7 for micronaire value, 8 for fiber elongation and 7 for fiber uniformity; and explained 3.4%-14.4%, 5.0%-42.4%, 6.5%0-11.7%, 5.1%-19.4%, and 6.9%-14.0% of the corresponding phenotypic variations, respectively. Analysis of QTL distribution indicated that D7, D8, and D9 chromosomes were enriched with QTLs controlling fiber strength in both parents, among them those existed in D7 and D8 chromosomes were closely linked and two QTLs controlling fiber strength located respectively in D7 and D9 chromosomes were in the complete same position, which could be related with that both 7235 and Yumian 1 bear the pedigree of elite fiber accession PD4381. The utilization potential of the QTL of super quality in the pyramiding breeding was also discussed.

Key words:Gossypium hirsutum,Fiber quality,QTL,Source

ReleaseDate:2014-07-21 14:20:31

[1] Beasley J O. The origin of the American tetraploid Gossypium species.Am Nat,1940,74:285-286

[2] Culp T W, Harrell D C, Kerr T. Some genetic implications in the transfer of high fiber strength genes to Upland cotton.Crop Sci,1979,19:481-483

[3] Culp T W. Simultaneous improvement of lint yield and fiber quality in upland cotton. In: Proceedings from Cotton Fiber Cellulose: Structure, Function, and Utilization Conference. Memphis, TN: National Cotton Council of American,1992.pp 247-288

[4] Qian S-Y(钱思颖), Huang J-Q (黄骏麒), Peng Y-J(彭跃进), Zhou B-L(周宝良), Ying M-C(应苗成), Shen D-Z(沈端庄), Liu G-L (刘桂玲), Hu T-X(胡廷馨), Xu Y-J(徐英俊), Gu L-M(顾立美), Ni W-C(倪万潮), Chen S(陈松). Study on hybridization of Gossypium hirsutum L. × G. anomalum Wawr. & Peyr. and application in breeding.Sci Agric Sin(中国农业科学),1992,25(6):44-51 (in Chinese with English abstract)

[5] Hu S-A(胡绍安), Cui R-X(崔荣霞), Wang K-B(王坤波), Wang C-Y(王春英), Yao C-B(姚长兵), Li S-H(黎绍惠). Studies on interspecific hybridization between the cultivated and the wild species in Gossypium and selection of new genotypes.Cotton Sci (棉花学报), 1993,5(2):7-13 (in Chinese with English abstract)

[6] Qian S-Y(钱思颖), Huang J-Q(黄骏麒), Zhou B-L(周宝良), Peng Y-J(彭跃进), Xu Y-J(徐英俊), Gu L-M(顾立美), Shen X-L(沈新莲). Study on hybridization of Gossypium hirsutum L. × G. klotzschianum Anderss and its uses.Jiangsu J Agric Sci(江苏农业学报),1996,12(4):18-22 (in Chinese with English abstract)

[7] Li S-C(李首成), Jiang X-Y (江先炎). Selecting high fiber strength and good quality cotton materials by utilizing interspecies hybrid from Gossypium hirsutum L. × G.arboreum L.China Cotton(中国棉花),1997,24(11):9-11 (in Chinese)

[8] Huang Z-K(黄滋康). Cotton Varieties and Their Genealogy in China (中国棉花品种及其系谱). Beijing: China Agriculture Press, 2007. p 154 (in Chinese)

[9] Liang Z-L(梁正兰). The Genetics and Breeding of Interspecific Hybridization in Cotton (棉花远缘杂交的遗传与育种). Beijing: Science Press,1999. pp 233-234 (in Chinese)

[10] Zhou B-L(周宝良), Chen S(陈松), Shen X-L(沈新莲), Zhang X-G(张香桂), Zhang Z-L (张震陵). Study on effect of three wild species for improving fiber quality in upland cotton (Gossypium hirsutum L.).Cotton Sci(棉花学报),2003,15(1):22-25 (in Chinese with English abstract)

[11] Reinisch A J, Dong J M, Brubaker C L, Stelly D M, Wendel J F, Paterson A H. A detailed RFLP map of cotton,Gossypium hirsutum × Gossypium barbadense: Chromosome organization and evolution in a disomic polyploid genome.Genetics,1994,138:829-847

[12] Yu J, Park Y H, Lazo R G, Kohel R J. Mapping cotton genome with molecular markers. In: Dugger P, Richter DA eds. Proceedings of the Beltwide Cotton Production Research Conferences. Memphis,TN: National Cotton Council of America, 1997. p 447

[13] Jiang C X, Wright R J, El-Zik K M, Paterson A H. Polyploid formation created unique avenues for response to selection in Gossypium (cotton).Proc Natl Acad Sci USA, 1998,95:4419-4424

[14] Kohel R J, Yu J, Park Y H. Molecular mapping and characterization of traits controlling fiber quality in cotton.Euphytica,2001,121:163-172

[15] Zhang T Z, Yuan Y L, Yu J, Guo W Z, Kohel R J. Molecular tagging of a major QTL for fiber strength in upland cotton and its marker-assisted selection.Theor Appl Genet,2003,106:262-268

[16] Mei M, Syed N H, Gao W, Thaxton P M, Smith C W, Stelly D M, Chen Z J. Genetic mapping and QTL analysis of fiber-related traits in cotton (Gossypium).Theor Appl Genet,2004,108:280-291

[17] Lacape J M, Nguyen T B, Courtois B, Belot J L, Giband M, Gourlot J P, Gawryziak G, Roques S, Hau B. QTL analysis of cotton fiber quality using multiple Gossypium hirsutum × Gossypium barbadense backcross generations.Crop Sci,2005,45:123-140

[18] Shen X L, Guo W Z, Zhu X F, Yuan Y L, Yu J, Kohel R J, Zhang T Z. Molecular mapping of QTL for fiber qualities in three diverse lines in upland cotton using SSR markers.Mol Breed,2005,15:169-181

[19] Zhang Z S, Xiao Y H, Luo M, Li X B, Luo X Y, Hou L, Li D M, Pei Y. Construction of a genetic linkage map and QTL analysis of fiber-related traits in upland cotton (Gossypium hirsutum L.).Euphytica,2005,144:91-99

[20] Wang J(王娟), Guo W-Z(郭旺珍), Zhang T-Z(张天真). QTL mapping for fiber quality properties in cotton cultivar Yumian1.Acta Agron Sin(作物学报),2007,33(12):1915-1921 (in Chinese with English abstract)

[21] Paterson A H, Brubaker C L, Wendel J F. A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP and PCR analysis.Plant Mol Biol Rep,1993,11:122-127

[22] Zhang J(张军), Wu Y-T(武耀庭), Guo W-Z(郭旺珍), Zhang T-Z(张天真). Fast screening of SSR markers in cotton with PAGE/silver staining.Cotton Sci(棉花学报),2000,12(5):267-269 (in Chinese with English abstract)

[23] Lander E S, Green P, Abrahmson J. MAPMAKER: An interactive computer package for constructing primary linkage maps of experimental and natural population.Genomics,1987,1:174-181

[24] Wang S C, Basten C J, Zeng Z B. Windows QTL Cartographer V2.0 Statistical Genetics, Bioinformatics Research Center, North Carolina State University. 2003. [2007-08-20]

[25] Zeng Z B. Precision mapping of quantitative trait loci.Genetics,1994,136:1457-1468

[26] Stuber C W, Edwards M D, Wendel J F. Molecular marker facilitated investigations of quantitative trait loci in maize: II. Factors influencing yield and its component traits.Crop Sci,1987,27:639-648

[27] Song X L, Wang K, Guo W Z, Zhang J, Zhang T Z. A comparison of genetic maps constructed from haploid and BC1 mapping populations from the same crossing between Gossypium hirsutum L × G. barbadense L.Genome,2005,48:378-390

[28] McCouch S R, Cho Y G, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T. Report on QTL nomenclature.Rice Genet Newsl,1997,14:11-13

[29] Ulloa M, Meredith W R Jr, Shappley Z W, Kahler A L. RFLP genetic linkage maps from four F2:3 populations and a join map of Gossypium hirsutum L.Theor Appl Genet,2002,104:200-208

[30] Lacape J M, Nguyen T B, Thibivilliers S, Bojinnov T B, Courtois B, Cantrell R G, Burr B, Hau B. A combined RFLP-SSR-AFLP map of tetraploid cotton based on a Gossypium hirsutum × Gossypium barbadense backcross population.Genome,2003,46:612-626

[31] Song X-L(宋宪亮), Sun X-Z(孙学振), Zhang T-Z(张天真). Segregation distortion and its effect on genetic mapping in plants.J Agric Biotechnol(农业生物技术学报),2006,14(2):286-292 (in Chinese with English abstract)

[32] Han Z G, Guo W Z, Song X L, Zhang T Z. Genetic mapping of EST-derived microsatellites from the diploid Gossypium arboreum in allotetraploid cotton.Mol Gen Genet,2004,272:308-327

[33] He D H, Lin Z X, Zhang X L, Nie Y C, Guo X P, Zhang Y X, Li W. QTL mapping for economic traits based on a dense genetic map of cotton with PCR-based markers using the interspecific cross of Gossypium hirsutum × Gossypium barbadense.Euphytica,2007,153:181-197

[34] Ulloa M, Cantrell R G, Percy R G, Zeiger E, Lu Z. QTL analysis of stomatal conductance and relationship to lint yield in intraspecific cotton.J Cotton Sci,2000,4:10-18