doi:

DOI: 10.3724/SP.J.1006.2014.00264

Acta Agronomica Sinica (作物学报) 2014/40:2 PP.264-272

Evaluation of Male-Sterility Induction Effect of Various Amino Acid Biosynthesis Inhibiting-Herbicides on Rapeseed (Brassica napus)


Abstract:
Oilseed rape is very sensitive to most sulfonylurea herbicides.The active constituent of sulfonylurea herbicides is acetolactate synthase (ALS), which catalyzes the common reaction to synthesize three branched-chain amino acids including leucine, valine, and isoleucine in plants.Some sulfonylurea herbicides have strong gametocidal effect on rapeseed.In the present paper, we evaluated the gametocidal effect of 26 herbicides inhibiting the biosynthesis of amino acids after leaf-spraying them at bolting stage of rapeseed (Brassica napus).Application of 6000 mg ha-1 glufosinate ammonium and 22 500 mg ha-1 glyphosate, inhibiting the biosynthesis of glutamine and phenylalanine respectively, caused partially male sterile.Except Bispyribac-sodium, Florasulam + flumetsulam, and Ethametsulfuron, the twenty-three ALS-inhibiting herbicides, including eighteen sulfonylureas, two imidazolinones, one sulfonylamino-carbonyltriazolinone, one triazolopyrimidines, and one pyrimidinylthio (or oxy)-benzoate, could induce male sterility in Brassica napus.Among them, Imazethapyr (600 mg ha-1), Pyrazosulfuron-ethyl (150 mg ha-1), Nicosulfuron (240 mg ha-1), Monosulfuron (200 mg ha-1), and Chlorsulfuron (120 mg ha-1) had high gametocidal efficiency which was not stable when the dosage changed slightly, indicating that they would be potential CHAs for Brassica napus by fine adjustment of dosage.Foliar application of Tribenuron-methyl or Amidosulfuron at the doses from 60 to 90 mg ha-1 on 20 Brassica napus varieties (lines) showed over 95% male sterility as well as lower phytotoxicity on pistil fertility.In conclusion, Tribenuron-methyl and Amidosulfuron are the best gametocides for Brassica napus among those herbicides.Our findings of gametocidal effect of those chemicals extend the function of ALS-inhibiting herbicides.

Key words:Brassica napus,Male sterility,Chemical hybridizing agents,Herbicide,Sulfonylurea

ReleaseDate:2015-09-12 18:10:57



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