DOI: 10.3724/SP.J.1006.2014.00581

Acta Agronomica Sinica (作物学报) 2014/40:4 PP.581-590

Comparison of Cadmium-Accumulation-Associated Genes Expression and Molecular Regulation Mechanism between Two Rice Cultivars (Oryza sativa L. subspecies japonica)

In plants, as in other eukaryotes, endogenous small interfering RNAs (siRNAs), a class of small non-coding RNAs, and DNA methylation regulate gene expression in developmental processes and adaptating to environmental stresses, including Cd stress. Cadmium (Cd) is a non-essential heavy metal and highly toxic to plants. To investigate the regulatory role of siRNAs and DNA methylation on genes involved in heavy metals transport, we compared these genes' expression profiles between a high Cd-accumulating rice (Oryza sativa L. subspecies japonica) cultivar (Xiushui 11) and a low Cd-accumulating rice cultivar (Xiushui 110). At five rice development stages investigated, the difference of these genes expression level between the two rice cultivars was not significant except OsPCR1, indicating OsPCR1 may be important in Cd transport in rice. Furthermore, quantita tive real time PCR (qRT-PCR) was performed to examine the expression level of a siRNA matched OsPCR1 second exon. Results indicated that the expression level of the siRNA negatively correlated with OsPCR1 expression level at the five stages. In addition, McrBC-qRT-PCR technology was used to determine DNA methylation level, showing that OsPCR1 expression level also negatively correlated with OsPCR1 second exon methylation level. These results of regulatory roles of siRNA and DNA methylation on OsPCR1 expression will contribute to the studies on OsPCR1 function and rice breeding for low Cd accumulation.

Key words:siRNA,DNA methylation,OsPCR1,Rice (Oryza sativa L.),Cadmium accumulation

ReleaseDate:2015-09-12 18:44:02

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