DOI: 10.3724/SP.J.1006.2014.00629

Acta Agronomica Sinica (作物学报) 2014/40:4 PP.629-635

QTL Mapping for Germination Percentage under Salinity and Drought Stresses in Brassica napus L. Using a SNP Genetic Map

The objective of this study was to identify QTLs for seed germination percentage under salinity and drought stresses in Brassica napus L. using a recombinant inbred line (RIL) population derived from the cross between yellow-seeded GH06 (female parent) and black-seeded P174 (male parent). The RILs were F11 developed via single-seed-descent propagation. QTLs for germination percentage under 16 g L-1 NaCl and 20% PEG-6000 (w/w) treatment, after four-day treatment and on the third and fourth day, were detected using the composite interval mapping (CIM) method. The linkage map contains 2795 SNP markers with the total length of 1832.9 cM and the average distance of 0.66 cM. A total of 19 QTLs were mapped on chromosomes A01, A03, A06, A07, A09, and C06. Twelve of them were identified under salinity stress with explained phenotypic variation from 4.9% to 10.9%, and eight QTLs were related to drought stress with explained phenotypic variation from 3.8% to 6.9%. The QTLs on A03 and A09 were located in close marker regions. Our results showed that the germination percentage of rapeseed is controlled by many minor-effect loci with great influence by environmental factors. Different genes are triggered in response to salinity or drought stress along with the stress duration.

Key words:Brassica napus,SNP,Germination percentage,Salinity stress,Drought stress,QTL

ReleaseDate:2015-09-12 18:44:03

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