DOI: 10.3724/SP.J.1006.2016.01419

Acta Agronomica Sinica (作物学报) 2016/42:10 PP.1419-1428

Interaction of Soybean 14-3-3 Proteins with Transcription Factor GmMYB173

14-3-3 proteins, nearly existing in all eukaryotic cells, may regulate many physiological and biochemical processes through interacting with other proteins. As the largest class of transcription factors in plants, MYB gene family is widely involved in plant growth and metabolism regulation. A gene cloned from soybean cultivar Zigongdongdou, and specifically expressed in the nuclear in the subcellular location assay. Sequence analysis showed that there was a binding site of 14-4-4 proteins, namely the pST binding site, in GmMYB173 similar to that in GmMYB176. The nuclear-specific expression of GmMYB173 disappeared when the sequence of the pST binding site was deleted through the splicing by overlap extension PCR. All 14-3-3 proteins from GmSGF14a to GmSGF14p could interact with GmMYB173. Among them, GmSGF14n interacted with GmMYB173 strongest, GmSGF14e and GmSGF14k took second place, which was proved by the β-galactosidase activity analysis. These results suggest that 14-3-3 proteins not only interact with GmMYB173, but also probably regulate its subcellular location. The information pro-vided by this study will facilitate the study of interaction relationship between 14-3-3 proteins and GmMYB173 and its function on the soybean development.

Key words:14-3-3 proteins,GmMYB173,Subcellular location,Yeast two-hybrid

ReleaseDate:2017-01-12 13:30:39

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