DOI: 10.3724/SP.J.1006.2016.01429

Acta Agronomica Sinica (作物学报) 2016/42:10 PP.1429-1436

Development and Characterization of TaMYB86-Overexpressing Transgenic Wheat Lines with Resistance to Common Root Rot

Wheat common root rot, mainly caused by Bipolaris sorokiniana, is a difficultly prevent soil-borne disease of wheat (Triticum aestivum L.) worldwide. TaMYB86, a B. sorokiniana-induced wheat MYB gene, encodes a MYB transcription factor. We constructed the TaMYB86 overexpression vector pUbi:MYC-TaMYB86 and introduced TaMYB86 into Yangmai 16 via the particle bombardment. The TaMYB86 transgenic wheat lines on generations of T0-T3 were underwent by molecular characteristics analysis and disease resistance evaluation. The PCR and quantitative RT-PCR results showed that the alien TaMYB86 was intro-duced into three transgenic wheat lines, and the relative transcriptional level of TaMYB86 was apparently higher in transgenic wheat lines than in non-transformed Yangmai 16. As Western blot results presented, the introduced MYC-TaMYB86 gene was translated into the MYC-TaMYB86 protein in the three overexpressing transgenic lines, but not in non-transformed Yangmai 16. The infection types and disease indexes of three TaMYB86 transgenic wheat lines were significantly lower than those of non-transformation Yangmai 16 (t-test, P < 0.01). The transcript levels of 3 wheat defense genes (PR10, PR17c, and Chit1) were significantly elevated in three transgenic wheat lines than in the non-transformed Yangmai 16. These results indicate that overex-pression of TaMYB86 enhances significantly resistance to B. sorokiniana in transgenic wheat lines and TaMYB86 plays a positive role in defense response to B. sorokiniana.

Key words:Triticum aestivum,MYB transcription factor,Defense response,Common root rot,Transgene

ReleaseDate:2017-01-12 13:30:40

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