DOI: 10.3724/SP.J.1006.2016.01448

Acta Agronomica Sinica (作物学报) 2016/42:10 PP.1448-1461

DNA Fingerprinting of Peanut (Arachis hypogaea L.) Varieties Released in Henan Province

The DNA fingerprints of 90 peanut varieties released in Henan province before 2015 were generated using 95 polymorphic loci of 14 SSR markers. All varieties were distinguished by the DNA polymorphisms except for three pairs of varieties with only one SSR locus difference between each pair. SSR clustering analysis revealed that the 90 peanut varieties could be classified into 88 types at a 0.98 genetic similarity coefficient. The remaining two variety pairs had only one allele difference since one variety was the maternal parent of the other. About three fourth (74.4%) of the peanut varieties were distinctive from other varieties at a genetic similarity coefficient threshold of 0.95, indicating a very relatively narrow genetic background of the released peanut varieties in Henan province compared to other crops. Population structure analysis based on 60 SSR markers demonstrated that the 90 varieties could be divided into three subgroups in accordance with the classification according to botani-cal and pod characteristics, which was consistent with the results of SSR markers cluster analysis. This study provides some fun-damental information for selection of parental lines, identification and evaluation of new varieties, as well as establishment of DNA fingerprinting standard in peanut breeding program.

Key words:Peanut variety,Fingerprinting,SSR,Population structure

ReleaseDate:2017-01-12 13:30:43

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