doi:

DOI: 10.3724/SP.J.1006.2016.01462

Acta Agronomica Sinica (作物学报) 2016/42:10 PP.1462-1470

Transcription Characteristics of ZmPHYA1 and ZmPHYA2 under Different Light Treatments in Maize


Abstract:
Plant phytochromes are a family of red/far-red light photoreceptors, which have two forms in plant: inactive red light absorbing form (Pr) and active far-red light absorbing form (Pfr). During plant growth and developmental processes, phytochromes play pivotal roles in regulations of seed germination, plant height, flowering time, and shade-avoidance. In the grasses, three subfamilies are present: PHYA, PHYB and PHYC. In maize, an ancient genome duplication has increased the family member to six: PHYA1, PHYA2, PHYB1, PHYB2, PHYC1, and PHYC2. Phytochrome A facilitates the inhibition of hypocotyl elongation, opening of the apical hook, expansion of cotyledons, accumulation of anthocyanin and blocking of greening by continuous FR (FRc) light. In order to evaluate the light response capability and difference of transcription abundance between ZmPHYA1 and ZmPHYA2, we employed quantitative real-time PCR (qRT-PCR) assay to investigate the expression patterns of ZmPHYA1 and ZmPHYA2 in the inbred line B73 and Mo17 with different light treatments. The results indicated that both ZmPHYA1 and ZmPHYA2 had a high expression level in leaf and silk, and the transcription abundance of ZmPHYA1 was 2-8 times higher than that of ZmPHYA2. Inbred lines of both B73 and Mo17 possessed longer mesocotyls in dark, far-red and blue light conditions than in red or white light conditions. Both ZmPHYA1 and ZmPHYA2 had a high expression level in far-red and blue lights and rapidly responded to dark-to-far-red and dark-to-blue transitions. ZmPHYA1 was more important under far-red light, so was ZmPHYA2 in blue light. Both of the genes could rapidly respond to transi-tions from dark to red or white light with similar expression pattern. The both genes also respond to long-day or short-day treatments, however the transcription abundance of ZmPHYA1 was 2-5 times higher than that of ZmPHYA2 during the treatments. All the results suggested that the transcription of both ZmPHYA1 and ZmPHYA2 could rapidly responded to different light treatments; ZmPHYA1 might be more effective than ZmPHYA2 in crop improvement. Our results provide a theoretical basis for the function study and evaluation of light response ability for both ZmPHYA1 and ZmPHYA2.

Key words:Maize,Phytochrome,Light signaling transduction,Expression analysis,Light treatment

ReleaseDate:2017-01-12 13:30:44



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