doi:

DOI: 10.3724/SP.J.1006.2016.01487

Acta Agronomica Sinica (作物学报) 2016/42:10 PP.1487-1494

Global Transcriptome Analysis in High- and Low-Nitrogen Responsive Inbred Lines of Maize


Abstract:
Different maize genotypes exhibit variation in nitrogen (N) responsiveness, however, the corresponding gene expression patterns are unexplored yet. Here, we performed a comprehensive transcriptome profiling of high- and low-nitrogen responsiveness genotypes in the conditions of sufficient-nitrogen (SN) and limiting-nitrogen (LN) supply. Under LN condition, B73, a high-N-responsive inbred line, accumulated a much lower N content in leaf than under SN condition. Whereas, the Mo17, a low-N-responsive inbred line, showed no significant difference in leaf N content between SN and LN. The RNAseq assay re-vealed a total of 13 867 and 10 028 differentially expressed genes in Mo17 and B73, respectively, including 9044 (Mo17) and 4233 (B73) up-regulated genes and 4823 (Mo17) and 5795 (B73) down-regulated under LN. Moreover, the principal component analysis (PCA) showed that the magnitude of transcriptome variation was greater in Mo17 than in B73 under LN. According to two-tail variance analysis, 342 differentially expressed genes had significant genotype-by-nitrogen interaction. These genes were mainly involved in amino acid metabolism, photosynthesis, biosynthesis of secondary metabolites, and gene replication and ex-pression. Our results suggest that more genes might be activated under N limiting condition in the low-N-responsive genotype than in the high-N-responsive genotype in order to modulate N assimilation and utilization in maize plant.

Key words:Maize,Nitrogen,Nitrogen stress,Transcriptome profiling,Nitrogen use efficiency (NUE)

ReleaseDate:2017-01-12 13:30:48



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