DOI: 10.3724/SP.J.1006.2016.01541

Acta Agronomica Sinica (作物学报) 2016/42:10 PP.1541-1550

Differential Proteomic Analysis and Photosynthetic Characteristics of Winter Rapeseed under Low Temperature Stress

Isolation and identification of the differentially expressed proteins in winter rapeseed at low temperature, laid a foundation for revealing the mechanism of cold resistance of winter rapa. The strong cold resistant winter rape Longyou 7 was used as experimental material. Two dimensional electrophoresis, mass spectrometry and retrieval techniques were used to compare the proteomic differences at low temperature (4℃, -4℃) and normal atmospheric temperature (25℃/20℃), and functions were analyzed by KO and KEGG. It was observed that Longyou 7 had subsided growth point, creeping stem, and closed or semi-closed stomata under low temperature. The 2-DE and PDQuest8.0.1 software analysis showed that the number of protein spots were 726 and 738, respectively. Compared with the normal temperature treatment, at the 4℃ treatment showed differential expression at 10 protein spots while didn't at five protein. Eleven proteins were identified by MS MALDI-TOF-TOF mass spectrometry analysis. They are involved in carbohydrate metabolism, rugan metabolism, amino acid metabolism, organic acid metabolism, nucleic acid metabolism, signal transduction in cell and communication and other cellular processes. we found the high activity of antifreeze protein in Longyou 7 leaf protein extraction solution after low temperature treatment through ice crystal morphology microscopic observation. Five out of the 11 proteins identified were associated with photosynthesis. Under low temperature, ribulose 1,5-bisphosphate carboxylase (RuBPCase) activity and photosynthetic rate decreases in leaves of Longyou 7. Under the low tem-perature, the proteome of winter rape was significantly changed, and the specific protein was expressed. The decrease of Pn in leaves was related to the expression inhibition and the activity decrease of RuBPCase. The decrease of Pn in leaves was mainly caused by non stomatal limitation. High activity of antifreeze protein plays an important role in cold resistance of winter rape.

Key words:Winter rape,Low temperature stress,Protein proteomics,Photosynthetic characteristics

ReleaseDate:2017-01-12 13:30:55

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