DOI: 10.3724/SP.J.1006.2017.01426

Acta Agronomica Sinica (作物学报) 2017/43:10 PP.1426-1433

Phenotypes and Gene Mapping of a Thermo-sensitive Yellow Leaf Mutant of Rice

Phenotypic analysis and gene mapping of rice leaf color could lay a foundation for map-based cloning of related genes and the function research of rice photosynthetic system. Leaf yellow mutant dy1 was obtained from rice cultivar "Nanjing 11" (abbreviated as NJ11) mutated by ethyl methanesulfonate (EMS). The mutant dy1 showed leaf yellowing at seedling stage and maturity stage in natural environment, with an abnormal structure of thylakoids under TEM, and significant differences in plant height, tiller number, seed setting rate and so on. The mutant dy1 showed albinism in 20℃, etiolation in 25℃ and virescence in 30℃. The rice leaf yellow mutant dy1 was controlled by a single recessive gene. F2 population was constructed by crossing the mutant with "02428", and the mutant phenotypes of extreme individuals were selected to map gene dy1. The gene was located in the 115 kb region of the long arm of chromosome 1, and contained 16 ORFs. Sequencing analysis showed that LOC_Os01g73450 controlling a uracil nucleotide kinase, with a single base substitution in the junction of the fourth intron and the fifth exon in dy1, might be a candidate gene. And the expression of genes related to chloroplast synthesis was significantly decreased, indicating dy1 may involve in the chloroplast synthesis

Key words:Rice,Thermo-sensitive,Genetic analysis,Leaf color,Gene mapping

ReleaseDate:2017-12-07 14:03:21

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