DOI: 10.3724/SP.J.1006.2017.01434

Acta Agronomica Sinica (作物学报) 2017/43:10 PP.1434-1447

Identification of QTLs Associated with High Yield of Super Rice Variety Zhongjiazao 17

Rice yield has obvious effect on national food security. The further excavation of high yield related genes is of great significance. In this research, a doubled haploid population (DH population) of Zhongjiazao 17 (YK17)×D50 was constructed by using the anther culture technique. The yield related traits including effective panicles per plant, grain number per panicle, seed setting rate and 1000-grain weight in three different planting environments (Lingshui, Hainan planting environment, high yield field in Hangzhou planting environment, low yield field in Hangzhou planting environment) were examined. Further, genetic linkage map of the DH population was constructed. A total of 74 QTLs for yield related traits were detected by QTL mapping, these QTLs were distributed on all the 12 chromosomes of rice with the contribution rate ranging from 3.7% to 43.2%. Among these QTLs, qPH1-1 and qFLL12 were detected repeatedly in three different planting environments, and the contribution rate of them was 8.9%, 24.2%, 43.2% and 16.6%, 17.9%, 18.9%, respectively. In addition, qPH3, qFLL10-2, qFLW11-1, qPL11, qGNP11, qSSR3, and qTGW5-1 were detected repeatedly in two different planting environments with the contribution rate ranging from 7.4% to 42.2%. The analysis of QTL×environment interaction showed that qPH1-1, qFLW2, qEPP1, and qTGW5-1 had significant additive×environment interaction effects. GW5 sequencing, located in qTGW5-1, indicated that GW5 alleles of high yield lines and low yield lines were inherited from the parents YK17 and D50, respectively, which was consistent with that the additive effect of qTGW5-1 derived from YK17. This research provides theoretical basis and technical support for further excavation of rice yield related genes and breeding super high yield varieties by using molecular polymerization breeding.

Key words:Yield related traits,QTL mapping,DH population,Zhongjiazao 17,Rice

ReleaseDate:2017-12-07 14:03:23

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