DOI: 10.3724/SP.J.1006.2017.01489

Acta Agronomica Sinica (作物学报) 2017/43:10 PP.1489-1498

Transcriptome Analysis of Promotive Effects of Active Carbon on Growth and Development of Maize Seedlings from in vitro Cultured Immature Embryos

Immature embryos from the maize inbred line Chang 7-2 were collected at 14 days after pollination, and cultured on MS or MSA medium (MS medium plus active carbon) for nine days at 24℃. Active carbon significantly accelerated the growth and development of maize seedlings from cultured immature embryos. Using RNA-seq technique, the genes involved in the growth promotive effects of active carbon were analyzed. The presence of active carbon in the medium affected the gene expression in seedlings significantly. Number of up-and down-regulated genes was 1612 and 530 in roots, as well as 69 and 78 in shoots, respectively; indicating that active carbon mainly affects gene expression in roots. GO enrichment analysis showed that differentially expressed genes (DEGs) in roots were mainly involved in DNA packaging, DNA packaging complex and hydrolase activity; the DEGs in shoots were mainly involved in lipid metabolic process, extracellular region and peroxidase activity. The KEGG enrichment analysis showed that the DEGs in roots were significantly associated with energy metabolism, carbohydrate metabolism, lipid metabolism, amino acid metabolism, cell cycle and plant hormone signal transduction. The DEGs in shoots were significantly associated with biosynthesis of ubiquinone and other terpenoid-quinone compounds. Several key genes involved in the cell cycle pathway (i.e., CYC, CDH1, MCM3, PCNA2, and BUB1), signal transduction of auxin (Aux/IAA) and cytochrome function (CYP450 oxidase) were significantly up-regulated by active carbon. Ten DEGs were confirmed by Real-time quantitative PCR assay, suggesting that our data and analysis of transcriptome sequencing are reliable.

Key words:Maize,Immature embryo,Active carbon,Transcriptome

ReleaseDate:2017-12-07 16:30:33

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