doi:

DOI: 10.3724/SP.J.1006.2018.00169

Acta Agronomica Sinica (作物学报) 2018/44:2 PP.169-176

Identification and Gene Mapping of a Floral Organ Number Mutant mf2 in Rice (Oryza sativa)


Abstract:
A rice floral organ mutant mf2 (multi-floret 2) was identified from Hanghui 7, an indica restorer line in rice (Oryza sativa L.), which was treated with heavy ion irradiation. The glumes, lodicules, stamens, pistil of the mutant increased, and most of the spikelets had 2-3 flowers. The palea and lemma of mf2 mutant were dehiscent, the shape and number of vascular bundles were different from those of the wild-type. Scanning electron microscope analysis revealed that the flowers variation of mf2 had been formed in the differentiation of the primordium during the spike differentiation stage. The heading date of the mf2 mutant was delayed, the plant height was decreased, but the number of panicles was increased, showing that the vegetative growth of the mutant was also affected. Genetic analysis showed that the mf2 mutant phenotype was controlled by a recessive nuclear gene. There are 321 recessive individuals from the F2 segregation population were used to fine map MF2. Finally, MF2 was located in a 102 kb physical interval between markers SSR39108 and InDel39210 on chromosome 1.

Key words:rice (Oryza sativa L.),heavy ion mutagenesis,floral mutants,gene mapping

ReleaseDate:2018-03-08 10:13:38



[1] Guo S, Sun B, Looi L S, Xu Y, Gan E S, Huang J, Ito T. Co-ordination of flower development through epigenetic regulation in two model species:rice and Arabidopsis. Plant Cell Physiol, 2015, 56:830-842

[2] Yanofsky M F. Floral meristems to floral organs:genes controlling early events in Arabidopsis flower development. Annu Rev Plant Physiol Plant Mol Biol, 1995, 46:167-188

[3] Fornara F, Marziani G, Mizzi L, Kater M, Colombo L. MADS-box genes controlling flower development in rice. Plant Biol, 2003, 5:16-22

[4] 田大刚, 刘华清, 苏军, 张礼华, 王锋. 水稻与拟南芥中控制花器官发育MADS-box基因的比较研究进展. 福建农业学报, 2011, 26:309-320 Tian D G, Liu H Q, Su J, Zhang L H, Wang F. Flower-Development-Controlling MADS-box genes in rice and Arabidopsis thaliana. Fujian J Agric Sci, 2011, 36:309-320(in Chinese with English abstract)

[5] Yoshida H, Nagato Y. Flower development in rice. J Exp Bot, 2011, 62:4719-4730

[6] Takahashi M, Nagasawa N, Kitano H, Nagato Y. Panicle phytomer 1 mutations affect the panicle architecture of rice. Theor Appl Genet, 1998, 96:1050-1056

[7] Kyozuka J, Konishi S, Nemoto K, Izawa T, Shimamoto K. Down-regulation of RFL, the FLO/LFY homolog of rice, accompanied with panicle branch initiation. Proc Natl Acad Sci USA, 1998, 95:1979-1982

[8] Jeon J S, Jang S, Lee S, Nam J, Kim C, Lee S H, Chung Y Y, Kim S R, Lee Y H, Cho Y G. Leafy hull sterile 1 is a homeotic mutation in a rice MADS box gene affecting rice flower development. Plant Cell, 2000, 12:871-884

[9] Chanhong K, Donghoon J, An G H. Molecular cloning and characterization of OsLRK1 encoding a putative receptor-like protein kinase from Oryza sativa. Plant Sci, 2000, 152:17-26

[10] Jang S, Lee B, Kim C, Kim S J, Yim J, Han J J, Lee S, Kim S R, An G. The OsFOR1 gene encodes a polygalacturonase-inhibiting protein (PGIP) that regulates floral organ number in rice. Plant Mol Biol, 2003, 53:357-369

[11] Suzaki T, Sato M, Ashikari M, Miyoshi M, Nagato Y, Hirano H Y. The gene FLORAL ORGAN NUMBER1 regulates floral meristern size in rice and encodes a leucine-rich repeat receptor kinase orthologous to Arabidopsis CLAVATA1. Development, 2004, 131:5649-5657

[12] Chu H, Qian Q, Liang W, Yin C, Tan H, Yao X, Yuan Z, Yang J, Huang H, Luo D. The FLORAL ORGAN NUMBER4 gene encoding a putative ortholog of Arabidopsis CLAVATA3 regulates apical meristem size in rice. Plant Physiol, 2006, 142:1039-1052

[13] Suzaki T, Toriba T, Fujimoto M, Tsutsumi N, Kitano H, Hirano H Y. Conservation and diversification of meristem maintenance mechanism in Oryza sativa:function of the FLORAL ORGAN NUMBER2 gene. Plant Cell Physiol, 2006, 47:1591-1602

[14] Zhao L, Xu S, Chai T, Tai W. OsAP2-1, an AP2-like gene from Oryza sativa, is required for flower development and male fertility. Plant Reprod, 2006, 19:197-206

[15] Lee D Y, Lee J, Moon S, Park S Y, An G. The rice heterochronic gene SUPERNUMERARY BRACT regulates the transition from spikelet meristem to floral meristem. Plant J, 2007, 49:64-78

[16] Sun Q, Zhou D X. Rice jmjC domain-containing gene JMJ706 encodes H3K9 demethylase required for floral organ development. Proc Natl Acad Sci USA, 2008, 105:13679-13684

[17] Li H, Xue D, Gao Z, Yan M, Xu W, Xing Z, Huang D, Qian Q, Xue Y. A putative lipase gene EXTRA GLUME1 regulates both empty-glume fate and spikelet development in rice. Plant J Cell Mol Biol, 2009, 57:593-605

[18] Xiao H, Tang J, Li Y, Wang W, Li X, Jin L, Xie R, Luo H, Zhao X, Meng Z. STAMENLESS 1, encoding a single C2H2 zinc finger protein, regulates floral organ identity in rice. Plant J, 2009, 59:789-801

[19] Ren D Y, Li Y F, Wang Z, Xu F F, Guo S, Zhao F M, Sang X C, Ling Y H, He G H.. Identification and gene mapping of a multi-floret spikelet 1(mfsl) mutant associated with spikelet development in rice. J Integr Agric, 2012, 11:1574-1579

[20] Wang N, Li Y F, Sang X C, Ling Y H, Zhao F M, Yang Z L, He G H. Nonstop glumes (nsg), a novel mutant affects spikelet development in rice. Genes & Genomics, 2013, 35:149-157

[21] Cai Q, Yuan Z, Chen M, Yin C, Luo Z, Zhao X, Liang W, Hu J, Zhang D. Jasmonic acid regulates spikelet development in rice. Nat Commun, 2014, 5:3476

[22] Zhang J, Tang W, Huang Y, Niu X, Zhao Y, Han Y, Liu Y. Down-regulation of a LBD-like gene, OsIG1, leads to occurrence of unusual double ovules and developmental abnormalities of various floral organs and megagametophyte in rice. J Exp Bot, 2015, 66:99-112

[23] Wang H H, Zhang L, Cai Q, Jin Z M, Zhao X X, Huang Q M, Luo Z J, Chen M J, Zhang D B, Yuan Z. OsMADS32 interacts with PI-like proteins and regulates rice flower development. J Integr Plant Biol, 2015, 57:504-513

[24] Yang C, Ma Y, Li J. The rice YABBY4 gene regulates plant growth and development through modulating the gibberellin pathway. J Exp Bot, 2016, 67:5545-5556

[25] 李云峰, 杨正林, 凌英华, 王楠, 任德勇, 王增, 何光华. 水稻多小花小穗突变体mf1的鉴定与基因定位. 作物学报, 2011, 37:280-285 Li Y F, Yang Z L, Ling Y H, Wang N, Ren D Y, Wang Z, He G H. Characterization and gene mapping of a spikelet mutant mf1 in rice. Acta Agron Sin, 2011, 37:280-285(in Chinese with English abstract)

[26] Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8:4321-4326

[27] Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis:a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88:9828-9832

[28] 赵利峰, 柴团耀. AP2/EREBP转录因子在植物发育和胁迫应答中的作用. 植物学通报, 2008, 25:89-101 Zhao L F, Chai T Y. Roles of AP2/EREBP family of transcription factors in development and stress response of plants, Chin Bull Bot, 2008, 25:89-101(in Chinese with English abstract)

[29] Poethig R S. Phase change and the regulation of developmental timing in plants. Science, 2003, 301:334-336

[30] Feng L, Gao Z, Xiao G, Huang R, Zhang H. Leucine-rich repeat receptor-like kinase FON1 regulates drought stress and seed germination by activating the expression of ABA-responsive fenes in rice. Plant Mol Biol Rep, 2014, 32:1158-1168

[31] Jiang L, Qian Q, Mao L, Zhou Q Y, Zhai W X. Characterization of the rice floral organ number mutant fon3. J Integr Plant Biol, 2005, 47:100-106

[32] Li Y, Xu P, Zhang H, Peng H, Zhang Q, Wang X, Wu X. Characterization and identification of a novel mutant fon(t) on floral organ number and floral organ identity in rice. J Genet Genomics, 2007, 34:730-737

[33] Kaplinsky N J, Freeling M. Combinatorial control of meristem identity in maize inflorescences. Development, 2003, 130:1149-1158

[34] Lee D Y, An G. Two AP2 family genes, SUPERNUMERARY BRACT (SNB) and OsINDETERMINATE SPIKELET 1(OsIDS1), synergistically control inflorescence architecture and floral meristem establishment in rice. Plant J, 2012, 69:445-461

[35] Padham A K, Hopkins M T, Wang T W, Mcnamara L M, Lo M, Richardson L G, Smith M D, Taylor C A, Thompson J E. Characterization of a plastid triacylglycerol lipase from Arabidopsis. Plant Physiol, 2007, 143:1372-1384

[36] Zhang B, Wu S, Zhang Y, Xu T, Guo F, Tang H, Li X, Wang P, Qian W, Xue Y. A high temperature-dependent mitochondrial lipase EXTRA GLUME1 promotes floral phenotypic robustness against temperature fluctuation in rice (Oryza sativa L.). PLoS Genet, 2016, 12:e1006152