doi:

DOI: 10.3724/SP.J.1006.2018.00297

Acta Agronomica Sinica (作物学报) 2018/44:2 PP.297-305

Homologous Cloning of BnGS3 and BnGhd7 Genes in Brassica napus and Their Relationship with Rapeseed Yield-related Traits


Abstract:
Brassica napus is an allotetraploid with complex genomic structure, but Brassica genome has a collinearity with that of rice. In this study, the homologous genes BnGS3 and BnGhd7 of Brassica napus were obtained by homologous cloning of the yield related genes GS3 and Ghd7 of rice. BnGS3 has six exons with a 666 bp of ORF and encodes 222 amino acids. BnGS3 protein has VWF structure, one of the four conserved domains of rice GS3, and belongs to type A. BnGhd7 contains one exon having ORF with a full-length of 1014 bp and encoding 337 amino acids. BnGhd7 protein has two important domains, the N-terminal B-Box and the C-terminal CCT. BnGS3 and BnGhd7 were located in linkage groups A2 and A10, respectively. Polymorphism markers brgs-16 of BnGS3 and polymorphism markers brghd-3 and ghd7-7 of BnGhd7 were obtained by comparative sequencing, among which brghd-3 with thousand kernel weight (P < 0.05) and ghd7-7 with plant height (P <0.01) were positively correlated, and ghd7-7 was negatively correlated with flowering stage (P < 0.05). The results indicate that it is feasible to clone the homologous genes of Brassica napus using rice functional gene sequence information, which provides an effective way in Brassica napus functional gene research.

Key words:Brassica napus,homologous cloning,yield,comparative sequencing,correlation analysis

ReleaseDate:2018-03-08 10:13:40



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