DOI: 10.3724/SP.J.1006.2019.83058

Acta Agronomica Sinica (作物学报) 2019/45:5 PP.656-661

Genetic analysis and causal gene identification of maize viviparous mutant vp-like8

The maize mutant vp-like8 shows clear viviparous phenotype and stable inheritance, and genetic analysis showed that the mutant phenotype was controlled by a single recessive gene. Using an F2 segregation population derived from vp-like8 and inbred line Zheng 58, the causal gene was mapped to an interval from 160.4 Mb to 165.6 Mb on chromosome 3 by the BSR-Seq technology. According to the maize genomic database, a previously discovered viviparous gene Vp1 was identified to be in this mapping interval. The test crosses from vp1 and vp-like8 heterozygous plants showed a 3:1 segregation ratio between normal and viviparous kernels. The genomic sequence analysis revealed that vp-like8 mutant had a 343 bp deletion in the second intron and 222 bp insertion in the third intron of Vp1 gene, which is different from vp1 mutation of an only 343 bp deletion in the second intron of Vp1 gene. Further real time PCR analysis revealed that, compared with the normal kernels, the transcript level of Vp1 was significantly decreased both in vp-like8 and vp1 viviparous kernels. Taken together, these evidences suggest that vp-like8 is a new allele mutant of Vp1.

Key words:maize,viviparous,mutant,Vp1,gene mapping

ReleaseDate:2019-11-05 15:29:58

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