DOI: 10.3724/SP.J.1006.2019.82049

Acta Agronomica Sinica (作物学报) 2019/45:5 PP.662-675

Phenotypic identification and gene mapping of temperature-sensitive green-revertible albino mutant tsa2 in rice (Oryza sativa L.)

Temperature-sensitive leaf color mutants of rice are ideal materials in studies on photosynthesis, chloroplast structure and function, and chloroplast development. A temperature-sensitive green-revertible albino mutant (tsa2) with genetically stable mutational traits was screened out from the progeny of ethyl methane sulfonate (EMS) treated indica three-line maintainer line Xinong 1B. The wild type seedlings had normal phenotype at 22℃, while the mutant tsa2 had completely albino leaves and about 40% of albino seedlings died at the seedling stage; the photosynthetic pigment contents and photosynthetic rate of surviving albino seedlings decreased significantly, and the main agronomic traits were significantly lower than those of the wild type at maturity stage. When germinated at 28℃, tsa2 showed light-green leaves with white streaks and significantly lower photosynthetic pigment contents than the wild type, while a small difference of photosynthetic rate and main agronomic traits between the tsa2 and the wild type. No significant difference in leaves was observed between tsa2 and the wild type when seedlings germinated at 32℃. Transmission electron microscope observation revealed that the albino leaves of tsa2 demonstrated abnormal chloroplast development (without differentiated grana and granum lamella) or without chloroplast at 22℃ and completely developed chloroplasts in partial mesophyll cells at 28℃, and normal number and morphology of mesophyll cells compared with wild type at 32℃. The analysis of qRT-PCR indicated that genes related to partial photosynthetic pigment metabolism pathways, chloroplast development and photosynthesis expressed in tsa2 to a varying degrees compared with these of the wild type. Genetic analysis suggested that mutational phenotype of tsa2 was controlled by a single recessive nuclear gene, TSA2, which was finally mapped between SSR markers S5-57 and S5-119 on chromosome 5, with a physical distance of 718 kb. These results lay a foundation for the research on genetic improvement and the mechanism explanation of chloroplast development affected by temperature in rice (Oryza sativa L.).

Key words:rice (Oryza sativa L.),temperature-sensitive,green-revertible albino,chloroplast ultrastructure,gene mapping

ReleaseDate:2019-11-05 15:29:59

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