DOI: 10.3724/SP.J.1006.2019.84121

Acta Agronomica Sinica (作物学报) 2019/45:5 PP.705-713

Comparative analysis on flower bud differentiation of multilocular and bilocular traits in Brassica juncea

Brassica juncea, possessing natural multilocular traits compared with bilocular rapeseed (two-chambered), has high yielding potential due to multiple locules (3-5 locules) and multi-grains. To disclose the formation process of multilocular traits, we constructed a BC3F5 population from a cross between Qinghai multilocular rapeseed and Tayou 2 (two locules) to analyse the whole process of flower bud differentiation of homozygous bilocular plants and multilocular plants through paraffin section along with scanning electron-microscopy observation. The flower bud differentiation in multilocular rapeseed took place at about 22 days after seedling emergence, with an average leaf number of 5, and whole differentiation was divided into five stages. The diameters of shoot apical meristem (SAM) and floral meristem (FM) in multilocular plants were significantly larger than these in bilocular plants. The center of growing cone of the multilocular was deeply recessed into an "X" shaped region with four carpels, while that of the bilocular into an "I" shape, forming half and half regions with two carpels, such differentiation could affect the number of carpels and seeds rows. The tip of the stigma also formed "I" and "X" shapes, which could be used as an indicator, to distinguish between multilocular plants and bilocular plants in the early stage.

Key words:multilocular,flower bud differentiation,shoot apical meristem,floral meristem,carpel

ReleaseDate:2019-11-05 15:30:00

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