doi:

DOI: 10.3724/SP.J.1006.2019.84104

Acta Agronomica Sinica (作物学报) 2019/45:5 PP.792-797

Repetitive intense flashes inhibit photosystem II activity and thermal dissipa-tion in cotton leaves


Abstract:
Not only continuous high light results in the decrease of photosynthetic efficiency, but also intense flashes may affect the photosynthetic function. In this study, cotton (Gossypium hirsutum L.) cultivar Xinluzao 45 was used to investigate the effects of repetitive intense flash treatment (leaves exposed to 20,000 μmol m-2 s-1 for 300 ms, with interval time of 10 s, and the whole treatment duration was 30 min) on two photosystems and photosynthetic function of cotton leaves. Chlorophyll fluorescence, P700 and gas exchange were measured before and after repetitive intense flash treatment, respectively. The content of active PSI (photosystem I) reaction center and the electron transfer activity of PSⅡ (photosystem Ⅱ) all decreased after repetitive intense flash treatment which reflected by the significant increase in J and K phases of the fluorescence induction kinetics curves after repetitive intense flash treatment. φND (the quantum yield of non-photochemical energy dissipation due to donor side limitation) of PSI decreased while φNA (the quantum yield of non-photochemical energy dissipation due to acceptor side limitation) increased, indicating that acceptor side of PSI was primarily inhibited by repetitive intense flashes. Repetitive intense flash treatment induced a distinct decrease in the quantum yield of PSⅡ in cotton leaves under actinic light. Moreover, φNPQ (the quantum yield of regulated energy dissipation) of PSⅡ decreased significantly after repetitive intense flash treatment. However, φNO (the quantum yield of non-regulated energy dissipation) increased considerably, demonstrating that the repetitive intense flashes caused PSⅡ photoinhibition. The photosynthetic rate and the stomatal conductance decreased while the intercellular CO2 concentration increased after repetitive intense flash treatment, indicating that the reduction of carbon assimilation induced by repetitive intense flash treatment is not limited by stomata. Therefore, we believe that the repetitive intense flash treatment not only induces inactivation of PSI, but also leads to PSⅡ photoinhibition and the decrease of thermal dissipation. The suppression of photosynthetic electron transport activity may play important role in the decrease of photosynthetic rate after repetitive intense flash treatment.

Key words:cotton,repetitive intense flashs,photoinhibition,photosynthesis,electron transport

ReleaseDate:2019-11-05 15:30:04



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