doi:

DOI: 10.3724/SP.J.1006.2019.84110

Acta Agronomica Sinica (作物学报) 2019/45:5 PP.798-805

Screening and comprehensive evaluation of aluminum-toxicity tolerance during germination stage in 52 varieties (lines) of pea germplasm


Abstract:
With increasing acidification of soil, aluminum toxicity has become one of the important stress factors affecting seed germination quality and crop yield. The purpose of this study is to explore the characteristics of aluminum toxicity tolerance in different genotypes of Pisum sativum, and establish the comprehensive evaluation system for screening and breeding new varieties with aluminum toxicity tolerance. The identification of tolerance at germination stage was performed according to the comprehensive tolerance coefficient of aluminum toxicity (CAC) value, the average subordinate function value (ASF value), the comprehensive evaluation value of aluminum toxicity tolerance (A-value) in correlation analysis, frequency analysis, principal component analysis, cluster analysis and stepwise regression analysis. Fifty-two pea varieties collected from different places were treated with 40 mg L-1 Al3+ screened in the pretest. There were significant differences between treatment and control groups in germination rate, germination energy, germination index, root length, bud length, root dry weight, bud dry weight, root-shoot ratio, and the genetic diversity index ranging from 1.43-2.03 and 1.51-2.06, respectively, showing extensive genetic variation. Cluster analysis was carried out based on A value, exhibiting that the tested cultivars were roughly divided into groups with three aluminum toxicity tolerance grades. The first group including two varieties is tolerant to aluminum toxicity, the second group including 19 varieties is sensitive to aluminum toxicity, and the last group containing 31 other varieties is very sensitive to aluminum toxicity. Combined with the optimal regression equation, seven indexes of pea seed germination rate, germination potential, germination index, bud length, root length, bud dry weight and root dry weight were used to comprehensively evaluate and identify the aluminum toxicity tolerance of pea during germination period. Through comprehensive evaluation and cluster analysis, germplasm C175 and C145 were screened out with strong tolerance to aluminum toxicity in germinating stage, which can be used as important resources for breeding near varieties and studying the mechanism of tolerance to aluminum toxicity in pea.

Key words:pea,germination period,aluminum toxicity tolerance,comprehensive evaluation,germplasm resources screening

ReleaseDate:2019-11-05 15:30:04



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