doi:

DOI: 10.3724/SP.J.1006.2009.01474

Acta Agronomica Sinica (作物学报) 2009/35:8 PP.1474-1482

Comparison of Genetic Diversity between in-situ Conserved and Non-conserved Oryza rufipogon Populations in China


Abstract:
By the end of 2007, a total of 15 sites for Oryza rufipogon in-situ conservation had been established in different parts of China. However, these sites were selected only on the basis of the scientists’experience and the materials submitted by different provinces. Thus, the validity of the selected populations still needs to be verified. By virtue of 24 pairs of SSR markers, the re-search was conducted on genetic diversity for 427 accessions of Oryza rufipogon from the 15 in-situ conserved populations and 357 accessions from 15 non-conserved populations which were randomly selected according to their latitude, so as to clarify the genetic diversity and representativeness of the existing Oryza rufipogon in situ conserved populations in China. The results indicated that the average Ae and I of the 24 loci of the conserved populations were 5.98 and 1.90 respectively, which were higher than those of non-conserved populations (5.85 and 1.86 respectively). However, the average He of the 24 loci of the conserved populations was 0.79, which was lower than that of the non-conserved populations (0.80). Besides, test of significance on the Ae, I, and He of the SSR loci showed that there was no significant difference between the two populations, demonstrating that the genetic diversity of the conserved populations could be representative for the Oryza rufipogon populations in China. Moreover, the number of specific alleles of the conserved populations was 40, which far exceeded that of the non-conserved populations (20), showing that the conserved populations possessed more unique genes which are worth conserved. In addition, based on previous research findings and in accordance with the geographic information of the conserved populations, we found that the 15 conservation sites covered all the typical geographic types in the Oryza rufipogon distribution areas in China. Thus, we may conclude that the 15 in-situ conservation sites were scientifically and rationally selected. On the basis of the analysis of the genetic structure of the Oryza rufipogon populations in China, it was recommended that future in-situ conservation sites should be established mainly in the southern part of Guangxi and in Guangdong.

Key words:Oryza rufipogon Griff.,in-situ conservation,Genetic diversity,SSR

ReleaseDate:2014-07-21 14:47:09



[1] Zhong D-B(钟代彬), Luo L-J(罗利军), Ying C-S(应存山). Ad-vances on transferring e1ite gene from wild rice species into cul-tivated rice. Chin J Rice Sci (中国水稻科学), 2000, 14(2): 103-106 (in Chinese with English abstract)

[2] Xie J-K(谢建坤), Kong X-L(孔祥礼), Bao J-S(包劲松), Wan Y(万勇). Recent advances in molecular mapping and cloning of useful genes from wild rice and their application in breeding. Hereditas (遗传), 2004, 26 (1): 115-121 (in Chinese with Eng-lish abstract)

[3] Sun C Q, Wang X K, Li Z C, Yoshimura A. Comparison of the genetic diversity of common wild rice (Oryza rufipogon Griff.) and cultivated rice (O. sativa L.) using RFLP markers. Theor Appl Genet, 2001, 102: 157-162

[4] Zhu Z-F(朱作峰), Sun C-Q(孙传清), Fu Y-C(付永彩), Zhang P-J(张培江), Wang X-K(王象坤). Comparison of the genetic di-versity of common wild rice and cultivated rice using SSR mark-ers. Sci Agric Sin (中国农业科学), 2002, 35(12): 437-441 (in Chinese with English abstract)

[5] Zhang X-L(张晓丽), Guo H(郭辉), Wang H-G(王海岗), Lü J-Z(吕建珍), Yuan X-P(袁筱萍), Peng S-T(彭锁堂), Wei X-H(魏兴华). Comparative assessment of SSR allelic diversity in wild and cultivated rice in China. Acta Agron Sin (作物学报), 2008, 34(4): 591-597 (in Chinese with English abstract)

[6] Pang H-H(庞汉华), Chen C-B(陈成斌). Wild Rice Resources in China (中国野生稻资源). Guangxi: Guangxi Science and Tech-nology Press, 2001. pp 204-214 (in Chinese)

[7] Yang Q-W(杨庆文), Zhang W-X(张万霞), He D-X(贺丹霞), Chen D-Z(陈大洲), Dai L-Y(戴陆园), Chen C-B(陈成斌), Huang K-D(黄坤德). Studies on in-situ conservation methods of wild rice in China. J Plant Genet Resour (植物遗传资源学报), 2003, 4(1): 63-67(in Chinese with English abstract)

[8] Millar C I, Libby W J. Strategies for conserving clinal, ecotypic, and disjunct population diversity in widespread species. In: Falk D A, Holsinger K E, eds. Genetics and Conservation of Rare Plants. New York: Oxford University Press, 1991. pp 149-170

[9] Schaal B A, Leverisch W J, Rogstad S H. Comparison of meth-ods forassessing genetic variation in plant conservation biology. In: Falk D A, Holsinger K E, eds. Genetics and Conservation of Rare Plants. New York: Oxford University Press, 1991. pp 123-134

[10] Song Z P, Xu X, Wang B, Chen J K, Lu B R. Genetic diversity in the northernmost Oryza rufipogon populations estimated by SSR markers. Theor Appl Genet, 2003, 107: 1492-1499

[11] Gao L Z. Population structure and conservation genetics of wild rice Oryza rufipogon (Poaceae): A region-wide perspective from microsatellite variation. Mol Ecol, 2004, 13: 1009-1024

[12] Yu P(余萍), Li Z-C(李自超), Zhang H-L(张洪亮) , Li D-Y(李道远), Wang M-X(王美兴), Sun J-L(孙俊立), Wang X-K(王象坤). Genetic diversity of common wild rice (Oryza rufipogon Griff.) by using SSR markers and phenotypic traits in Guangxi province. Acta Genet Sin (遗传学报), 2004 , 31(9): 934-940 (in Chinese with English abstract)

[13] Li C(李晨), Pan D-J(潘大建), Mao X-X(毛兴学), Tu C-Y(涂从勇), Zhou H-Q(周汉钦), Fan Z-L(范芝兰), Li X-F(李晓方). Study on genetic diversity of Gaozhou wild rice by using SSR. Chin Sci Bull (科学通报), 2006, 51(5): 551-558 (in Chinese)

[14] Yang Q-W(杨庆文), Yu L-Q(余丽琴), Zhang W-X(张万霞), Chen D-Z(陈大洲), Shi J-X(时津霞), Ren J-F(任军方), Miao H(苗晗). Comparative studies on genetic diversities between in-situ and ex-situ conserved germplasm of Oryza rufipogon. Sci Agric Sin (中国农业科学), 2005, 38(6): 1073-1079 (in Chinese with English abstract)

[15] Li X-X(李小湘), Wang S-H(王淑红), Duan Y-H (段永红), Yu L-Q(余丽琴), Huang H-M(黄海明), Li W-H(李卫红), Sun G-H(孙桂华), Liu Y(刘勇). Comparative studies on the genetic diversity between conserved and non-conserved Oryza rufipogon populations. J Plant Genet Resour (植物遗传资源学报), 2007, 8 (4): 379-386 (in Chinese with English abstract)

[16] Qian J, He T H, Song Z P, Lu B R. Genetic evaluation of in-situ conserved and reintroduced populations of wild rice (Oryza rufi-pogon. Poaceae) in China. Biochem Genet, 2005, 12: 561-575

[17] Han D-F(韩东飞). Genetic Diversity and Conservation of Oryza rufipogon in China (中国普通野生稻的遗传多样性及其保护). MS Dissertation of Chinese Academy of Agricultural Sciences, 2006 (in Chinese with English abstract)

[18] Jin Y(金燕), Lu B-R(卢宝荣). Sampling strategies for genetic diversity. Biodiversity Sci (生物多样性), 2003, 11(2): 155-161 (in Chinese with English abstract)

[19] Guillmaut P, Drouard L M. Isolation of plant DNA: A fast, inex-pensive and reliable method. Plant Mol Biol Rep, 1992, 10: 60-65

[20] Panaud O, Chen X L, McCouch S R. Development of microsatel-lite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L). Mol Gen Genet, 1996, 252: 597-607

[21] Yeh F C, Yang R C, Boyle T. POPGENE Version 1.31 Quick User Guide. Canada: University of Alberta, and Center for Inter-national Forestry Research, 1999

[22] Pritchard J K, Stephens M, Donnelly P. Inference of population structure using multilocus genotype data. Genetics, 2000, 155: 945-959

[23] Falush D, Stephens M, Pritchard J K. Inference of population structure using multilocus genotype data: Linked loci and corre-lated allele frequencies. Genetics, 2003, 164: 1567-1587

[24] Falush D, Stephens M, Pritchard J K. Inference of population structure using multilocus genotype data: Dominant markers and null alleles. Mol Ecol Notes, 2007, 7: 574-578

[25] Evanno G, Regnaut S, Goudet J. Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study. Mol Ecol, 2005, 14: 2611-2620

[26] Li Y-F(李亚非), Chen C-B(陈成斌), Zhang W-X(张万霞), Liang S-C(梁世春), Yang Q-W(杨庆文). Studies on genetic diversity and population structure of Oryza rufipogon near the tropic of cancer in China. J Plant Genet Resour (植物遗传资源学报), 2007, 8(3): 280-284 (in Chinese with English abstract)

[27] Huang Y-J(黄英金), Li G-H(李桂花), Chen D-Z(陈大洲), Shi Q-H(石庆华), Xiao Y-F(肖玉峰), Wang F-J(王锋尖), Yu L-Q(余丽琴), Xiao Y-Q(肖叶青). A preliminary study on the genetic diversity of allozymicloci of Dongxiang wild rice. Acta Agric Univ Jiangxiensis (江西农业大学学报), 2002, 24(1): 1-5 (in Chinese with English abstract)

[28] Zhou H F, Xie Z W, Ge S. Microsatellite analysis of genetic di-versity and population genetic structure of a wild rice (Oryza rufipogon) in China. Theor Appl Genet, 2003, 107: 332-339

[29] Chen C-B(陈成斌). Studies on genetic diversity of Oryza rufi-pogon near the tropic of cancer in China. Subtrop Plant Sci (亚热带植物科学), 2001, 30 (4): 5-9 (in Chinese)

[30] Yang Q-W(杨庆文), Dai L-Y(戴陆园), Shi J-X(时津霞), Zhang W-X(张万霞), Ren J-F(任军方), Miao H(苗晗). Study of genetic diversity and conservation strategy of (Oryza rufipogon Griff.) in Yuanjiang. J Plant Genet Resour (植物遗传资源学报), 2004, 5(1): 1-5 (in Chinese with English abstract)

[31] Lin S-B(林色标), Chen L-H(陈丽华), Hong B-Y(洪彬艺). Characteristics and utilization value of (Oryza rufipogon Griff.) in Fujian. Fujian Sci Technol Rice & Wheat (福建稻麦科技), 2004, 22(4): 31-32 (in Chinese)

[32] Pang H-H(庞汉华), Chen C-B(陈成斌). Wild Rice Resources in China (中国野生稻资源). Guangxi: Guangxi Science and Tech-nology Press, 2001. pp 3-7 (in Chinese)

[33] Gao L-Z(高立志), Zhang S-Z(张寿洲), Zhou Y(周毅), Ge S(葛颂), Hong D-Y(洪德元). A survey of the current status of wild rice in China. Biodiversity Sci (生物多样性), 1996, 4(3): 160-166 (in Chinese)

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