DOI: 10.3724/SP.J.1004.2013.02043

Acta Automatica Sinica (自动化学报) 2013/39:12 PP.2043-2050

A Novel In-vivo Optical Projection Tomography System and Its Application

Optical projection tomography (OPT) is a low-cost and high-resolution imaging technology which can provide three-dimensional (3D) images for samples from 1 to 10mm. OPT has been greatly applied to drug efficacy evaluation or gene expression through imaging low scattering ex-vivo biological tissues, such as small animal extremities, animal embryos, etc. However, ex-vivo imaging cannot record dynamic change in biological tissues. Therefore, in-vivo application of OPT is promising. Our group has developed a novel OPT system for in-vivo imaging of small insects. The system consists of a continuous wave (CW) laser, three translation stages, a rotation stage, and a low-temperature electron multiplying (EM) CCD camera. The spatial resolution of our system is less than 10 μm with an imaging field between 1mm and 10mm. An imaging experiment costs less than 2 min containing a 5 s 3D reconstruction. Besides the system introduction, this paper also demonstrates the system operation processes and preliminary biological applications. Experimental results show that 3D in-vivo imaging of drosophila melanogaster pupae and other insects are easy to implement by using our system.

Key words:Optical projection tomography (OPT), in-vivo imaging, green fluorescent protein, drosophila melanogaster pupae, microscopic imaging

ReleaseDate:2014-07-21 17:04:34

Funds:National Natural Science Foundation of China (61172167, 81101084), Instrument Developing Project of the Chinese Academy of Sciences (YZ201164), Fellowship for Young International Scientists of Chinese Academy of Sciences (2010Y 2GA03), Supporting Foundation for University Key Youth Teacher of Heilongjiang Province of China (1155G12), Natural Science Foundation of Beijing (4111004), Visiting Professor-ship for Senior International Scientists of Chinese Academy of Sciences (2012T1G0036), Natural Science Foundation of Heilongjiang Province (F201311), and the Foundation of Heilongjiang Educational Committee (12531119)