DOI: 10.3724/SP.J.1143.2010.10004

Acta Botanica Yunnanica (云南植物研究) 2010/32:3 PP.189-203

Micro-morphological Characteristics of Leaf Epidermis under Light Microscopy and Its Taxonomic Significance in Persea(Lauraceae) from America

Leaf epidermal micro-morphological characters of 54 species belonging to genus Persea (Lauraceae) from America were comparatively studied by the means of light microscopy. It was shown that the leaf epidermal cells were usually regular bearing with straight or beaded anticlinal walls.Most of these species had unicellular trichomes uniformly distributed. The stomatal apparatus which were paracytic, only randomly presented in the areoles of abaxial epidermis for all sampled species, can be assigned into three types. The results showed that similar leaf epidermal characters can be found in the species with similar morphology. Nineteen characters were selected for PCA analysis, and the sampled species was divided into two clades. The characters of leaf epidermis were little different within a species but can vary among species. However, the multiple leaf epidermal characters including the shape of epidermal cells, the shape of stomatal apparatus, stomatal ledges and some distinct characters were relatively constant and taxonomically quite useful for the identification of some species with similar phenotypes.

Key words:America,Persea,Leaf epidermis,Micro-morphological characteristics

ReleaseDate:2014-07-21 15:19:50

Ben-Ya′acov A, 1995. The taxonomy of the avocado: a proposed new classification of the Persea subgenus Persea [R]. Third world Avocado Congress, Tel Aviv, Israel, Abstract: 75

Bergh BO, Ellstrand N, 1987. Taxonomy of the avocado [J]. California Avocado Society Yearbook 1987, 70: 135—145

Chen JQ(陈俊秋), Li L(李朗), Li J(李捷)et al, 2009. Bayesian inference of nrDNA ITS sequences from Machilus(Lauraceae)and its systematic significance [J]. Acta Botanica Yunnanica(云南植物研究),31 (2): 117—126

Christophel DC, Kerrigan R, Rowett AI, 1996. The use of cuticular features in the taxonomy of the Lauraceae [J]. Annals of the Missouri Botanical Garden, 83 (3): 419—432

Christophel DC, Rowett AI, 1996. Leaf and Cuticle Atlas of Australian Leafy Lauraceae [M]. Australian Biological Resources Study, Canberra

Dilcher DL, 1974. Approaches to the identification of angiosperm leaf remains [J]. The Botanical Review, 40: 1—157

Edwards HH, 1989. The stomatal complex of Persea borbonia [J]. Canadian Journal of Botany, 68: 2543—2547

Francisco GL, 2009. Persea pallescens, a new combination for Phoebe pallescens(Lauraceae, Perseeae), a mistaken taxon of Mexico and Guatemala [J]. Novon, 19 (2): 201—203

Franco C, 1939. Relation between chromosome number and stomata in Coffea [J]. Botany Garden, 100: 817—827

Hill RS, 1986. Lauraceous leaves from the Eocene of Nerriga, New South Wales [J]. Alcheringa, 10: 327—351

Kamel EA, Loutfy MHA, 2001. Laurel family (Lauraceae) the significance of cuticular features, petiole anatomy and SDS-PAGE in the taxonomy of the Lauraceae [J]. Pakistan Journal of Biological Science, 4 (9): 1094—1100

Kopp LE, 1966. A taxonomic revision of the genus Persea in the western hemisphere (Perseae-Lauraceae) [J]. Memoirs of New York Botany Garden, 14: 1—120

Kostermans AJGH, 1993. Mutisiopersea Kostermans, a new genus in Lauraceae [J]. Rheedea, 3: 132—135

Li J, Christophel DC, 2000. Systematic relationships within the Litsea complex (Lauraceae): A cladistic analysis based on morphological and leaf cuticle data [J]. Australian Systematic Botany, 13: 1—13

Li J, Christophel DC, Conran JG et al, 2004. Phylogenetic relationships within the ‘core’ Laureae (Litsea complex, Lauraceae) inferred from sequences of the chloroplast gene matK and nuclear ribosomal DNA ITS regions [J]. Plant Systematics and Evolution, 26: 19—36

Meissner CF, 1864. Lauraceae [A]. In: Candolle AD ed., Prodromus Systematic Naturalis Regni Vegetavilis [M], 15: 1—260

Mhameed S, Sharon D, Kaufman D et al, 1997. Genetic relationships within avocado (Persea americana Mill) cultivars and between Persea species [J]. Theoretical and Applied Genetics, 94: 279—286

Nishida S, Christophel DC, 1999. Leaf anatomy of Beilschmiedia (Lauraceae) in the neotropics [J]. Nature and Human Activities, 4: 9—43

Nishida S, van der Werff H, 2007. Are cuticular characters useful in solving generic relationships of problematic species of Lauraceae? [J].Taxon, 56 (4): 1229—1237

Pole M, 2007. Lauraceae macrofossils and dispersed cuticle from the Miocene of southern New Zealand [J]. Palaeontologia Electronica,10 (1): 3A—38p

Rohwer JG, Li J, Rudolph B et al, 2009. Is Persea (Lauraceae) monophyletic? Evidence from nuclear ribosomal ITS sequences [J]. Taxon, 58 (4): 1153—1167

Rojas EC, Terrazas T, Lo′pez-Mata L, 2007. Persea (avocados) phylogenetic analysis based on morphological characters: hypothesis of species relationships [J]. Genetic Resources and Crop Evolution, 54: 249—258

van der Werff H, 2002. A synopsis of Persea (Lauraceae) in central America [J]. Novon, 12 (4): 575—586

Wilkinson HP, 1979. The Plant Surface (Mainly Leaf) [A]. Metcalfe CR, Chalk L eds., Anatomy of the Dicotyledons [M]. Oxford: Clarendon Press, 1: 97—165

Williams LO, 1977. The avocados, a synopsis of the genus Persea, subgPersea [J]. Economic Botany, 31: 315—320

Zhuang XY(庄雪影), Zhang Y(张粤), Sun TX(孙同兴), 2002. Leaf epidermis and their taxonomic significance in Machilus of Hong Kong [J]. Journal of South China Agricultural University(华南农业大学学报), 23 (1): 52—54