doi:

DOI: 10.3724/SP.J.1143.2010.10001

Acta Botanica Yunnanica (云南植物研究) 2010/32:3 PP.211-220

Species Diversity of Russuloid Mycorrhizae-forming Fungi on Pinus yunnanensis Seedlings and the Mycorrhizal Morphology


Abstract:
Russuloid ectomycorrhizae on 1-2 years-old seedlings of Pinus yunnanensis collected from the central Yunnan, China were investigated. Six morphotypes were recognized by macro- and anatomical-morphological approaches as well as molecular analyses. The six morphotypes were confirmed to represent six phylotypes by matching rDNA ITS sequences. The fungal partners in the six morpho-phylotypes are those closely related with Russula livescens, R.violeipes, R.densifolia, R.nigricans, R.sanguinea and R.nauseosa respectively. The correlation between the morphology of mantles and cystidia and the phylogenetic clades is further supported in our work. The framework, in which this report is included, shows that russuloid mycorrhizae are one of the most dominant representative ectomycorrhizae formed on 1-2 years seedlings of Pinus yunnanensis.

Key words:Pinus yunnanensis,Russula,Ectomycorrhizae,ITS,Morphotypes

ReleaseDate:2014-07-21 15:19:51



金振洲, 彭鉴, 2004. 云南松 [M]. 昆明: 云南科学技术出版社

Agerer R, 1987-2006. Colour Atlas of Ectomycorrhizae [M]. Einhorn-Verlag, Schwbisch Gmünd

Agerer R, 1991. Characterization of ectomycorrhiza [J]. Methods in Mycrobiology, 23: 26—65

Agerer R, 2001. Exploration types of ectomycorrhizae: a proposal to classify ectomycorrhizal mycelial systems according to their patterns of differentiation and putative ecological importance [J]. Mycorrhiza, 11: 107—114

Agerer R, 2006. Fungal relationships and structural identity of their ectomycorrhizae [J]. Mycological Progress, 5 (2): 67—107

Beenken L, 2004a. Les ectomycorhizes du genre Russula [J]. Bulletin of Society mycology of France, 120(1-4): 293—333

Beenken L, 2004b. Die Gattung Russula Untersuchungen zu ihrer Systematik anhand von Ektomykorrhizen [M]. dissertation zur erlangung des grades eines doktors, der Naturwissenchaften, der Fakultat Biologie, der Ludwig-Maximilians-Universitat München

Dai KJ(戴开结), Shen YX(沈有信), Zhou WJ(周文君) et al., 2005. Rhizosphere pH of Pinus yunnanensis Franch. and rhizosphere pH of P.yunnanensis seedlings at different phosphrous rates [J]. Acta Bot Boreal-Occident Sin(西北植物学报), 25(12): 2490—2494

Dai KJ(戴开结), He F(何方), Shen YX(沈有信) et al., 2009. Effects of different phosphorus on growth and P absorption of Pinus yunnanensis Franch. seedlings [J]. Acta Ecologica Sinica(生态学报), 29(8): 4078—4083

Deng Y(邓云), Guan HL(官会林), Dai KJ(戴开结) et al., 2006. Effects of phosphorus supply on seedling morphology and organic acids exudation of Pinus yunnanensis [J]. Journal of Yunnan University(云南大学学报), 28(4): 358—363

Gao YQ(高永茜), Zhou YH(周跃华), Tian K(田昆) et al., 2009a. Cultivating Pinus yunanensis seedlings with bagasse substrates [J]. Journal of Zhejiang Forestry College(浙江林学院学报), 26(4): 598—602

Gao YQ(高永茜), Zhou YH(周跃华), Zheng W(郑畹) et al., 2009b. Seedling raising experimental study on Pinus yunnanensis of different families [J]. Journal of West China Forestry Science(西部林业科技), 38(2): 47—52

Gardes M, Bruns D, 1993. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rust [J]. Molecular Ecology, 2: 113—118

Gardes M, Bruns TD, 1996. Community structure of ectomycorrhizal fungi in a Pinus muricata forest: above- and below-ground views [J]. Canadian Journal of Bonaty, 74: 1572—1583

Haug I, Wei M, Homeier J et al., 2005. Russulaceae and thelephoraceae form ectomycorrhizas with members of the nyctaginaceae (Caryophyllales) in the tropical mountain rain forest of southern Ecuador [J] . New Phytologist, 165: 923—936

Horton TR, Bruns TD, 2001. The molecular revolution in ectomycorrhizal ecology: peeking into the black-box [J]. Molecular Ecology, 10: 1855—1871

Ingleby K, Munro RC, Noor M et al., 1998. Ectomycorrhizal populations and growth of Shorea parvifolia (Dipterocarpaceae) seedling regenerating under three different forest canopies following logging [J]. Forest Ecology and Management, 111: 171—179

Koljalg U, Larsson KH, Abarenkov K et al., 2005. UNITE: A database providing web-based methods for the molecular identification of ectomycorrhizal fungi [J]. New Phytologist, 166: 1063—1068

Li GX(李贵祥), Shi HJ(施海静), Meng GT(孟广涛) et al., 2007. Community structural properties and species diversity in primary Pinus yunnanensis forest [J]. Journal of Zhejiang Forestry College(浙江林学院学报), 24(4): 396—400

Liu DY(刘代亿), LI GQ(李根前), Zheng W(郑畹) et al., 2008. Discussion on key problems of excellent clone selection of Pinus yunnanensis at early stage [J]. Forest Inventory and Planning(林业调查规划), 33(3): 67—72

Matsuda Y, Noguchi Y, Ito SI, 2009. Ectomycorrhizal fungal community of natually regenerated Pinus thunbergii seedlings in a coastal pine forest [J]. Japan of Forestry Research, 14: 335—341

Miller SL, Buyck B, 2002. Molecular phylogeny of the genus Russula in europe with a comparison of modern infrageneric classifications [J]. Mycological Research, 106(3): 259—276

Nunez MA, Horton TR, Simberloff D, 2009. Lack of belowground mutualisms hinders Pinaceae invasions [J]. Ecology, 90(9): 2352—2359

Rathnayake AP, Kadono H, Toyooka S et al., 2008. A novel optical interference technique to measure minute root elongations of Japanese red pine (Pinus densiflora Seibold & Zucc.) seedings infected with ectomycorrhizal fungi [J]. Environmental and Experimental Botany, 64: 314—321

Sarnari M, 1998. Monografia Illustrata del Genre Russula in Europa [M]. Tomo primo, Associazione Micologica Bresadola, Trento

Shimono Y, Kato M, Takamatsu S, 2004. Molecular phylogeny of Russulaceae (Basidiomycetes; Russulales) inferred from the nucleotide sequences of nuclear large subunit rDNA [J]. Mycoscience, 45: 303—316

Sonthworth D, Carrington EM, 2009. Mycorrhizas on nursery and field seedlings of Quercus garryana [J]. Mycorrhiza, 19: 149—158

Tedersoo L, Jairus T, Horrton BM et al., 2008. Strong host preference of ectomycorrhizal fungi in a tasmanian wet sclerophyll forest as revealed by DNA barcording and taxon-specific primer [J]. New Phytologist, 180: 479—490

Yu FQ(于富强), Liu PG(刘培贵), 2005. Species diversity of wild edible mushrooms from Pinus yunnanensis forests and conservation strategies [J]. Biodiversity Science(生物多样性), 13(1): 58—69

Yu FQ(于富强), Xiao YQ(肖月芹), Liu PG(刘培贵), 2007. Spatiotemporal distribution of ectomycorrhizal fungi in Pinus yunnanensis forests [J]. Acta Ecologica Sinica(生态学报), 27(6): 2305—2333

Zhang YM(张跃敏), Li GQ(李根前), Li LF(李莲芳) et al., 2008. The growth characterstics of Pinus yunnanensis and improvement measures [J]. Shanxi Forest Science and Techology(陕西林业科技),(3): 4—7

Zhang YM(张跃敏), Li GQ(李根前), Li LF(李莲芳) et al., 2009. Fertilization effect of nitrogen combination with phosphorus on seedling growth of Pinus yunnanensis [J]. Journal of Southwest Forestry University(西南林学院学报),(3): 5—10

Zhong K(仲凯), Liu HX(刘红霞), 2008. The new characters and application of mycorrhizal studies [J]. Ecological Sciences(生态科学), 27(3): 169—178

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