doi:

DOI: 10.3724/SP.J.1035.2012.01142

Acta Hydrobiologica Sinica (水生生物学报) 2012/36:6 PP.1142-1148

ECOTOXICOLOGICAL EFFECTS OF EXPOSURE TO NONYLPHENOL ON COURTSHIP BEHAVIOR OF ZABRAFISH DANIO RERIO


Abstract:
Endocrine disrupting chemicals (EDCs) pose serious risks to the health of animals. As an important and susceptible indicator in examining and assessing ecotoxicological effects of EDCs in aquatic ecosystem, fish behavior is of high ecological significance. Animals are always behaving. Behavioral repertoire represents the consequence of a diversity of physiological and biochemical alterations. It is a link between physiological responses to contaminants and ecological processes for a given species. Behavioral change is one of the most noticeable endpoints produced by EDCs, as toxicants can often disrupt or even eliminate the behavioral patterns that are essential for the fitness and survival of the entire population. However, it has been largely overlooked by ecotoxicologists until the last a few years. Nonylphenol (NP) has been extensively used in industrial and household production and is commonly released from industrial and municipal sewage treatment plants into the aquatic ecosystem. NP is one of the most serious environmental concerned EDCs and is capable of mimicking the biochemical behavior of natural estrogen and interacting with estrogen receptor. Courtship behavior in fish is hormonally controlled, thus it may serve as a sensitive indicator of sublethal exposure to estrogenic compounds. If courtship behavior is suppressed by EDCs, spawning potential would be depressed. Therefore, it is important to evaluate the effects of EDCs on courtship behavior of fishes. However, a standard protocol for assess-ing the effect of EDCs on courtship behavior in zebrafish (Danio rerio) has not yet been well established. In this study, the effects of exposure to nonylphenol (NP) on courtship behavior and fecundity of zebrafish (Danio rerio) were exam-ined to evaluate the possible hazard of NP to courtship behavior of fish and to pursue a potential biomarker of NP con-tamination. Fish were randomly divided into six groups. Five groups were exposed to 0.1, 1, 10, 50 and 100 μg/L nominal concentrations of NP for 24d, respectively. The sixth (control) group was exposed to acetone only. Acetone concentration in water did not exceed 10 μL/L. The contaminants were administered via the water, as 50% of the water was exchanged daily and replaced with water contaminated with NP. Courtship behavior was observed during a 15min period. The frequency of chasing, pushing, tail-nose and quiver was used to quantify the courtship behavior performance of male zebrafish, and the fecundity was studied to assess reproductive success. NP exposure showed remarkably effect on fecundity (P = 0.005). A positive correlation (P<0.05) was found between the fecundity and the frequency of chasing (R = 0.292), pushing (R = 0.293), tail-nose (R = 0.377) and quiver (R = 0.362). No significant differences in the fre-quency of chasing, pushing, tail-nose, quiver behavior were observed in different NP exposure groups, whereas NP im-posed marked influence on the reproductive effort of chasing (P = 0.017) and tail-nose behavior (P = 0.021). The re-productive effort of chasing and tail-nose behavior in zebrafish might be promising ecologically relevant biomarker or indicator of NP contamination in aquatic ecosystem.

Key words:Nonylphenol,Zebrafish,Courtship behavior,Reproductive effort,Ecotoxicological effect

ReleaseDate:2014-07-21 16:42:40



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