DOI: 10.3724/SP.J.1105.2014.13225

Acta Polymerica Sinica (高分子学报) 2014/014:1 PP.173-178

One-pot Preparation of Bioactive Surface by Electropolymerization of Dopamine

An electrochemical technique involved facile method of cyclic voltammetric oxidation of dopamine to fabricate thickness controllable PDA films (ePDA) was reported. This process was preceded under a neutral environment and in the absence of oxygen. Most importantly, with precisely controlled electrochemical parameters, this dopamine electropolymerization technique can be further combined with bioactive molecules modification via a one-pot strategy to construct functionalized surfaces. By electropolymerization of dopamine in a one-pot mixture with REDV peptide, an endothelial cells (ECs) specific ligand, ECs adhesion enhanced bioactive surface incorporated with REDV was obtained. Ellipsometry and scanning electron microscopy (SEM) confirmed the homogeneous ePDA film fabrication. X-ray photoelectron spectroscopy (XPS) and fluorescence analysis results demonstrated the successful incorporation of REDV peptide into ePDA films (ePDA@REDV). Cells adhesion experiments suggested an excellent activity of incorporated REDV peptide was retained. Furthermore, ECs exhibited improved attachment, spreading and vinculin formation on ePDA@REDV functionalized surface. This dopamine electropolymerization assisted one-pot strategy for rapid construction of bioactive surfaces provides an innovative approach for multifunctional modification of complicated conductive biomaterials and devices.

Key words:Electropolymerization, Polydopamine, REDV peptide, One-pot, Endothelial cells

ReleaseDate:2014-07-21 17:05:25

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