DOI: 10.3724/SP.J.1105.2014.13213

Acta Polymerica Sinica (高分子学报) 2014/014:2 PP.270-275

Biodegradable PLGA Microspheres for Controlled Delivery of Parathyroid Hormone Related Peptide

PLGA microspheres were chosen as the carrier to overcome the limitations of application for parathyroid hormone related peptide (PTHrP) which can increase the number and viability of osteoblasts and promote bone formation with a low-dose,long time,intermittent administration.PLGA microspheres were prepared by a water-in-oil-in-water (W1/O/W2) double emulsion solvent evaporation and PTHrP1-34 was encapsulated to the microspheres by a technique with good sphericity and high efficiency.The micrographs of polyester microspheres were evaluated by scanning electron microscope (SEM).The polyester microspheres had a good spherical shape.PTHrP1-34 loaded microspheres exhibited a slow release behavior for about 25 days,which was accord with expection.The effect of PTHrP1-34 loaded microspheres on pre-osteoblastic proliferation was studied via cell proliferation evaluation experiments.The results demonstrated that PTHrP1-34 loaded PLGA microspheres can enhance the proliferation and differentiation of MC3T3 cell effectively.Alkaline phosphatase (ALP) quantification experiment was also performed to investigate the influence of differentiation.The cells incubated with PTHrP1-34 loaded PLGA microspheres exhibited higher ALP level which was related to the differentiation.In summary,PTHrP1-34 loaded PLGA microspheres show great potential as a promising delivering and releasing carriers for application of oral implantology.

Key words:Parathyroid hormone related peptide, PLGA, Microspheres, Controlled-release

ReleaseDate:2014-07-21 17:05:31

1 Canalis E.Clinical Endocrinology & Metabolism,2010,95(4):1496~1506

2 Blind E.Clinical Laboratory,2008,54(11):439~449

3 Pleiner D J,Zwettler E,Paschalis E.Calcified Tissue International,2009,84(3):159~170

4 Chen C,Koh A J,Datta N S.J Biol Chem,2004,279:29121~29129

5 Devogelaer J P,Boutsen Y,Manicourt D H.Current Osteoporosis Report,2010,8(3):154~161

6 Inoue D,Matsumoto T.Biomed Pharma-cother,2000,54:32s~41s

7 Horwitz M J,Tedesco M B,Gundberg C.J Clin Endocrinol Metab,2003,88:569~575

8 Stewrat A F,Cain R L,Burr D B.J Bone Miner Res,2000,15(8):1517~1525

9 Jiang G,Matsumoto H,Fujii A.J Dent Res,2007,86:52~57

10 Yang J,Farnell D,Devlin H.J Dent Res,2005,33:123~129

11 Li Cong(李聪),Zhang Zhe(张喆),He Chaoliang(贺超良),Zhuang Xiuli(庄秀丽),Chen Li(陈莉),Cai Qing(蔡青),Xue Yan(薛妍),Zhang Bin(张宾),Wang Shizhen(王世振),Meng Weiyan(孟维艳),Chen Xuesi(陈学思).Acta Polymerica Sinica(高分子学报),2012,(7):778~783

12 Vahle J L,Sato M,Long G.Toxicol Pathol,2002,30(3):312~321

13 Cann C,Oe E,Sanchez S,Arnaud C.J Bone Miner Res,2000,66(6):456~460

14 Lu Rong(陆荣),Zhang Feng(张峰),Ding Cuicui(丁翠翠),Li Suya(李苏亚).Technology & Development of Chemical Industry(化工技术与开发),2010,39(9):4~7

15 Jung R E,Cochran D L,Domken O,Seibl R,Jones A A,Buser D,Hammerle C H.Clin Oral Implants Res,2007,18(3):319~325

16 Xiong Lei(熊雷),Jiang Hongwei(姜宏伟),Wang Dizhen(王迪珍).Acta Polymerica Sinica(高分子学报),2008,(8):791~796

17 Shi Xudong(石旭东),Sun Lei(孙磊),Gan Zhihua(甘志华).Acta Polymerica Sinica(高分子学报),2011,(8):866~873

18 Zhang Xiumei(张秀梅),Cai Jing(蔡静),Yang Yanan(杨亚楠),Chen Xuesi(陈学思),Xie Xuming(谢续明).Acta Polymerica Sinica(高分子学报),2011,(6):653~660

19 Alves E C,Szejnfeld V L,Pereira S N,Coelho L E,Heldan M C.Calcif Tissue Int,2010,87(5):424~436