doi:

DOI: 10.3724/SP.J.1105.2010.00250

Acta Polymerica Sinica (高分子学报) 2010/010:2 PP.250-254

PREPARATION OF HIGH MOLECULAR WEIGHT SOY PROTEIN AQUEOUS SOLUTION AND SEPARATION OF ITS MAIN COMPONENTS


Abstract:
Both solubility tests and reference data proved that commercially available soy protein isolate (SPI) had low solubility in pure water.As an alternative,guanidine hydroxyl chloride (GuHCl) and dithiothreitol (DTT) were used to treat SPI during the dissolution process.After dialysis of GuHCl and DTT,an SPI aqueous solution was obtained.Solubility tests showed this SPI aqueous solution had a relatively high concentration (6 times larger as that directly dissolved in pure water),and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that such an SPI solution did not suffer the degradation of the protein subunits.In addition,through a simple treatment of GuHCl and dialysis process,a 7S-rich solution and an 11S-rich sediment were obtained,so that the two major components of SPI were primarily separated.SDS-PAGE gave the major evidence of the separation,and the possible mechanism of the separation was discussed.It was a relative simple method with high productivity, though the separation efficiency was lower than that of the chromatography method.The paper provides new insights into the role of disulfide bonds that play in stabilizing the structure of SPI,especially that of the component 11S.

Key words:Glycinin, β-Conglycinin, Dialysis, Disulfide bond, Hydrogen bond

ReleaseDate:2014-07-21 15:07:40



1 Chen Nina(陈妮娜),Chen Riyao(陈日耀),Zheng Xi(郑曦),Chen Xiao(陈晓),Chen Zhen(陈震).Acta Polymerica Sinica(高分子学报),2008,(11):1068~1075

2 Huang Zhihai(黄志海),Li Jihong(李纪宏),Lin Pinghua(林萍华),Dong Yinsheng(董寅生),Guo Chao(郭超),Sheng Xiaobo(盛晓波),Chu Chenglin(储成林).Acta Polymerica Sinica(高分子学报),2009,(3):273~277

3 Cheng Cheng(程成),Shao Zhengzhong(邵正中),Chen Xin(陈新).Acta Polymerica Sinica(高分子学报),2008,(10):974~978

4 Liu Xiaoya(刘晓亚),Jiang Peng(姜鹏),Zhou Hua(周华),Jiang Jinqiang(江金强),Bai Huiyu(白绘宇),Jiang Ming(江明).Acta Polymerica Sinica(高分子学报),2009,(5):430~436

5 Kumar R,Choudhary V,Mishra S,Varma I K,Mattiason B.Ind Crops Prod,2002,16:155~172

6 Zhang X F,Min B G,Kumar S.J Appl Polym Sci,2003,90:716~721

7 Lu Y S,Weng L H,Zhang L N.Biomacromolecules,2004,5:1046~1051

8 Riblett A L,Herald T J,Schmidt K A,Tilley K A.J Agric Food Chem,2001,49:4983~4989

9 Utsumi S,Maruyama N,Satoh R,Adachi M.Enzyme Microb Technal,2002,30:284~288

10 Adachi M,Takenaka Y,Gidamis A B,Mikami B,Utsumi S.J Mol Biol,2001,305:291~305

11 Adachi M,Ho C Y,Utsumi S.J Agric Food Chem,2004,52:5717~5723

12 Tian Kun(田琨),Guan Juan(管娟),Shao Zhengzhong(邵正中),Chen Xin(陈新).Progress in Chemistry(化学进展),2008,20:565~573

13 Wolf W J,Babcock G E,Smith A K.Arch Biochem Biophys,1962,99:265~274

14 Wu S W,Murphy P A,Johnson L A,Reuber M A,Fratzke A R.J Agric Food Chem,2000,48:2702~2708

15 Chove B E,Grandison A S,Lewis M.J Food Hydrocolloids,2007,21:1379~1388

16 Thanh V H,Shibasaki K J.Agric Food Chem,1976,24:1117~1121

17 Ishino K,Okamoto S.Cereal Chem,1975,52:9~21

18 Sathe S K.J Food Biochem,1991,15:33~49

19 Maruyama N,Sato R,Wada Y,Matsumura Y,Goto H,Okuda E,Nakagawa S,Utsumi S.J Agric Food Chem,1999,47:5278~5284

20 Iwabuchi S,Yamauchi F.J Agric Food Chem,1987,35:205~209

21 Arrese E L,Sorgentini D A,Wagner J R,Anon M C.J Agric Food Chem,1991,39:1029~1032

22 Greene R F,Pace C N.J Biol Chem,1974,249:5388~5393

23 Santoro M M,Bolen D W.Biochemistry,1988,27:8063~8068

24 Loo J A,Edmonds C G,Udseth H R,Smith R D.Anal Chem,1990,62:693~698

25 Chen Xin(陈新),Shao Zhengzhong(邵正中),Zhou Li(周丽).China patent(中国专利),Pat,No.CN1483866.2004-03-24

26 Sze K W C,Kshirsagar H H,Venkatachalam M,Sathe S K.J Agric Food Chem,2007,55:8745~8753

27 Wolf W J.J Agric Food Chem,1993,41:168~176

PDF