doi:

DOI: 10.3724/SP.J.1085.2013.00208

Advances in Polar Science 2013/24:4 PP.208-212

Diversity and bioactivity of actinomycetes from Signy Is-land terrestrial soils,maritime Antarctic


Abstract:
The Antarctic represents a largely untapped source for isolation of new microorganisms with potential to produce bioactive natural products.Actinomycetes are of special interest among such microorganisms as they are known to produce a large number of natural products,many of which have clinical,pharmaceutical or agricultural applications.We isolated,characterized and classified actinomycetes from soil samples collected from different locations on Signy Island,South Orkney Islands,in the maritime Antarctic.A total of 95 putative actinomycete strains were isolated from eight soil samples using eight types of selective isolation media.The strains were dereplicated into 16 groups based on morphology and Amplified Ribosomal DNA Restriction Analysis(ARDRA)patterns.Analysis of 16S rRNA gene sequences of representatives from each group showed that streptomycetes were the dominant actinomycetes isolated from these soils;however,there were also several strains belonging to diverse and rare genera in the class Actinobacteria,including Demetria,Glaciibacter,Kocuria,Marmoricola,Nakamurella and Tsukamurella.In addition,screening for antibacterial activity and non-ribosomal peptide synthetase genes showed that many of the actinomycete strains have the potential to produce antibacterial compounds.

Key words:actinomycete diversity,Antarctica,rare actinomycetes,non-ribosomal peptide synthetase genes

ReleaseDate:2015-04-16 13:27:15



1 Stackebrandt E, Rainey F A, Ward-Rainey N L. Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacte-riol, 1997, 47(2): 479-491.

2 Aislabie J M, Chhour K L, Saul D J, et al. Dominant bacteria in soils of Marble Point and Wright Valley, Victoria Land, Antarctica. Soil Biol Bio-chem, 2006, 38(10): 3041-3056.

3 Babalola O O, Kirby B M, Roes-Hill M L, et al. Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils. Environm Microbiol, 2009, 11(3): 566-576.

4 Cary C S, McDonald I R, Barrett J E, et al. On the rocks: the microbiology of Antarctic Dry Valley soils. Nature Rev Microbiol, 2010, 8(2): 129-138.

5 Schumann P, Prauser H, Rainey F A, et al. Friedmanniella antarctica gen. nov., sp. nov., an LL-diaminopimelic acid-containing actinomycete from Antarctic sandstone. Int J Syst Bacteriol, 1997, 47(2): 278-283.

6 Mevs U, Stackebrandt E, Schumann P, et al. Modestobacter multiseptatus gen. nov., sp. nov., a budding actinomycete from soils of the Asgard Range (Transantarctic Mountains). Int J Syst Evol Microbiol, 2000, 50(1): 337-346.

7 Li H R, Yu Y, Luo W, et al. Marisediminicola antarctica gen. nov., sp. nov., an actinobacterium isolated from the Antarctic. Int J Syst Evol Microbiol, 2010, 60(Pt 11): 2535-2539.

8 Cui X L, Mao P H, Zeng M, et al. Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol, 2001, 51(Pt 2): 357-363.

9 Prabahar V, Dube S, Reddy G S, et al. Pseudonocardia antarctica sp. nov. an actinomycetes from McMurdo Dry Valleys, Antarctica. Syst Appl Microbiol, 2004, 27(1): 66-71.

10 Guglielmin M, Ellis-Evans C J, Cannone N. Active layer thermal regime under different vegetation conditions in permafrost areas. A case study at Signy Island (Maritime Antarctica). Geoderma, 2008, 144(1-2): 73-85.

11 Holdgate M W. Signy Island. Phil Trans R Soc Lond B Biol Sci, 1967, 252(777): 173-177.

12 Smith R I L. Signy Island as a paradigm of biological and environmental change in Antarctic terrestrial ecosystems // Kerry K R, Hempel G Antarctic ecosystems: ecological change and conservation. Berlin Heidelberg: Springer Verlag, 1990: 32-50.

13 Pearce D A, van der Gast C, Lawley B, et al. Bacterioplankton community diversity in a maritime Antarctic lake, determined by culture-dependent and culture-independent techniques. FEMS Microbiol Ecol, 2003, 45(1): 59-70.

14 Chong C W, Tan G Y A, Wong R C S, et al. DGGE fingerprinting of bacteria in soils from eight ecologically different sites around Casey Station, Antarctica. Polar Biol, 2009, 32(6): 853-860.

15 Chong C W, Pearce D A, Convey P, et al. High levels of spatial heterogeneity in the biodiversity of soil prokaryotes on Signy Island, Antarctica. Soil Biol Biochem, 2010, 42(4): 601-610.

16 Ayuso-Sacido A, Genilloud O. New PCR Primers for the screening of NRPS and PKS-I systems in actinomycetes: Detection and distribution of these biosynthetic gene sequences in major taxonomic groups. Microb Ecol, 2005, 49(1): 10-24.

17 Ronald M A. Handbook of microbiological media. New York: CRC Press, 1993.

18 Küster E, Williams S T. Selection of media for isolation of streptomycetes. Nature, 1964, 202(4935): 928-929.

19 Sambrook J F, Russell D W. Molecular cloning: A laboratory manual, 3rd edn. New York: Cold Spring Harbor Laboratory Press, 2001.

20 Lane D J. 16S/23S rRNA sequencing // Stackerbrandt E, Goodfellow M. Nucleic acid techniques in bacterial systematics. New York: Wiley & Sons, 1991: 115-175.

21 Altschul S F, Gish W, Miller W, et al. Basic local alignment search tool. J Mol Biol, 1990, 215(3): 403-410.

22 Chun J, Lee J H, Jung Y, et al. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol, 2007, 57(10): 2259-2261.

23 Tamura K, Dudley J, Nei M, et al. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol, 2007, 24(8): 1596-1599.

24 Nedialkova D, Naidenova M. Screening the antimicrobial activity of ac-tinomycetes strains isolated from Antarctica. J Cult Collect, 2005, 4(1): 29-35.

25 Nichols D S, Sanderson K, Buia A, et al. Bioprospecting and biotechnology in Antarctica // Jabour-Green J, Haward M. The Antarctic: Past, Present & Future. Hobart: Antarctic CRC, 2002: 85-103.

26 Teng H B, Tang H X, Xiao H, et al. Isolation and identification of actino-bacteria from rhizospheric soil in the Arctic Yellow River Station. Chinese J Polar Res, 2009, 21(1): 33-42 (in Chinese).

27 Sanyika W T, Stafford W, Don Cowan A. The soil and plant determinants of community structures of the dominant actinobacteria in Marion Island terrestrial habitats, Sub-Antarctica. Polar Biol, 2012, 35(8): 1129-1141.

28 Peeters K, Hodgson D A, Convey P, et al. Culturable diversity of hetero-trophic bacteria in Forlidas Pond (Pensacola Mountains) and Lundstrom Lake (Shackleton Range), Antarctica. Microb Ecol, 2011, 62(2): 399-413.

29 Pearce D A, Newsham K K, Thorne M A, et al. Metagenomic analysis of a southern maritime Antarctic soil. Front Microbiol, 2012, 3: 403.