DOI: 10.3724/SP.J.1148.2011.00126

Arid Zone Research (干旱区研究) 2011/28:1 PP.126-132

Osmolyte Accumulation of Armeniaca vulgaris under Continuous Drought Stress

In this paper, the osmotic regulation characteristics of 4 types of Armeniaca vulgaris seedlings under 80-day drought stress were studied. Drought stress treatment was applied to test the osmotic regulation characteristics of biennial seedlings of Armeniaca vulgaris f. aurantica L., Wang et D. F. Cui and Armeniaca vulgaris f. glyeyosma L., Wang et D. F. Cui, and the relative soil moisture content at depth of 15-20 cm, relative water content in leaves of A. vulgaris and the contents of Na+, K+, soluble protein, soluble sugar and proline were measured every 20 days. The results show that the relative soil moisture content at depth of 15-20 cm was decreased to slight drought stress (54%) after the seedlings were irrigated for 20 days, the relative water content in leaves was maintained higher than 80% except that in leaves of sweet kernel No. 2. Under slight drought stress, K+ accumulation occurred in leaves of most wild apricots. Na+ accumulation occurred in leaves of all four types of wild apricot seedlings when the relative soil moisture content was decreased to the severe drought stress (47%-18%) after the seedlings were irrigated for 40-80 days. Soluble protein was relatively sensitive to drought stress, it was accumulated under slight to moderate drought stress, and such accumulation was possibly related to the drought-resistant capability of seedlings. Soluble sugar was also accumulated when drought stress was severer. Proline content was sharply increased under severe drought stress after the seedlings were irrigated for 60 days, but it was too low to contribute to osmotic regulation under hyperosmotic conditions, which suggests that the Proline accumulation is a symptom that the cells were injured under such drought stress.

Key words:wild apricot,drought stress,soluble protein,soluble sugar,Ili valley,Xinjiang

ReleaseDate:2014-07-21 15:54:41

[1] 林培钧, 崔乃然.天山野果林资源——伊犁野果林综合研究[M].北京: 中国林业出版社, 2000.[Lin Peijun,Cui Nairan.Wild Fruit Forest Resources in Tianshan Mountains—Comprehensive Research on Wild Fruit Forest in Ili,Xinjiang,China[M].Beijing:Chinese Forestry Publishing House,2000.]

[2] 侯冬花, 萨拉木·艾尼瓦尔, 海利力·库尔班,等.伊犁不同类型野生杏花期冻害及坐果率研究[J].新疆农业科学, 2007,44(2): 122-125.[Hou Donghua,Salam·Aniwar,Halil·Kurban,et al.Study on the freezing injury and the fruit set percentage in flowering stage of different types of wild apricot in Ili[J].Journal of Xinjiang Agrecultural Sciences, 2007,44(2):122-125.]

[3] 李新周, 刘晓东, 马柱国.近百年来全球主要干旱区的干旱化特征分析[J].干旱区研究,2004, 21(2):97-103.[Li Xinzhou,Liu Xiaodong,Ma Zhuguo.Analysis on the drought characteristics in the main arid regions in the world since hundred-odd years[J].Arid Zone Research, 2004,21(2):97-103.]

[4] Ghannoum O.C4 photosynthesis and water stress[J].Annals of Botany,2009,103:635-644.

[5] Martinez J P,Kinet J M,Bajji M,et al.NaCl alleviates polyethylene glycol-induced water stress in the halophyte species Atriplex halimus L[J].Journal of Experimental Botany,2005,56(419): 2 421-2 431.

[6] 刘海燕, 李吉跃, 赵燕, 等.干旱胁迫对5个种源沙柳(Salix psammophila)气体交换及水分利用效率的影响[J].干旱区研究, 2007, 24(6): 815-820.[Liu Haiyan,Li Jiyao,Zhao Yan,et al.Influence of drought stress on gass exchange and water use efficiency of Salix psammophila in five places[J].Arid Zone Research,2007, 24(6): 815-820.]

[7] Fitter A H,Hay RKM.Environmental Physiology of Plants[M].London:Academic Press ,1987:121-184.

[8] Morgan J M.Osmoregulation and water stress in higher plants[J].Annual Review of Plant Physiology,1984,35:299-319.

[9] Johan A H.Osmoregulation[J].Plant Physiology,1976,27:485-505.

[10] Hasegawa P M,Bressan R A,Zhu J K,et al.Plant cellular and molecular responses to high salinity[J].Annual Review of Plant Physiology and Plant Molecular Biology,2000,51:463-499.

[11] 蒲光兰, 袁大刚, 胡学华, 等. 杏树抗旱性研究[J].西北林学院学报,2005,20(3): 40-43. [Pu Guanglan,Yuan Dagang,Hu Xuehua,et al.Characteristics of Prunus armeniaca against drought[J].Journal of Northwest Forestry University,2005,20(3):40-43.]

[12] 彭立新,李德全,束怀瑞.园艺植物水分胁迫生理及耐旱机制研究进展[J].西北植物学报,2002,22(5):1 275-1 281.[Peng Lixin,Li Dequan,Su Huairui.Advances in horticultural plant water stress physiology and drought resistance mechanism research[J].Acta Botanica Boreali-Occidentalia Sinica,2002,22(5):1 275-1 281.]

[13] 周海燕.金昌市4种乔木抗旱性生理指标的研究[J].中国沙漠, 1997,17(3): 301-303.[Zhou Haiyan.Study on the drought resistance physiological indexes of four kind of tree plant in Jinchang city[J].Journal of Desert Research,1997,17(3): 301-303.]

[14] Farrant JM,Cooper K,Kruger LA,et al.The effect of drying rate on the survival of three desiccation-tolerant angiosperm species[J].Annals of Botany,1999,84:371-379.

[15] Earl H J.A precise gravimetric method for simulating drought stress in pot experiments[J].Crop Science,2003,43:1 868-1 873.

[16] 王蕾, 海利力·库尔班, 萨拉木·艾尼瓦尔. 野生杏种子对外源赤霉素的生理响应[J].干旱区研究, 2009, 26(5): 708-713.[Wang Lei,Halil Kurban,Salam Aniwar.Physiological response of wild apricot seeds to exogenous gibberelin[J].Arid Zone Reserch,2009,26(5):708-713.]

[17] 张宪政. 作物生理研究法[M]. 北京: 农业出版社, 1999. [Zhang Xianzheng.Methods of Crop Physiological Research[M].Beijing:Agricultural Press, 1999.]

[18] Pearcy R W,Ehleringer J,Mooney H A,et al.Plant Physiological Ecology: Field Methods and Instrumentation[M].London:Published by Chapman and Hall,1994.

[19] Kurban H,Saneoka H,Nehira K,et al.Effect of salinity on growth and accumulation of organic and inorganic solutes in the leguminous plants Alhagi pseudoalhagi and Vigna radiata[J].Soil Science Plant Nutrition,1998,44:589-597.

[20] Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding[J].Analytical Biochemistical,1976,72:248-254.

[21] Bates L S,Waldren R P,Teare I D.Rapid determinaton of free proline for water-stress studies[J].Plant and Soil,1973,39:205-207.

[22] Morgan J M.Osmoregulation and water stress in higher plants[J].Annual Review of Plant Physiology,1984,35:299-319.

[23] Cushman JC.Osmoregulation in plants:Implications for agriculture[J].Integrative and Comparative Biology,2001,41:758-769.

[24] Maathuis FJM,Sanders D.Mechanisms of potassium absorption by higher plant roots[J].Physiologia Plantarum,1996,96:158-168.

[25] Lacombe B,Pilot G,Michard E,et al.A shaker-like K+ channel with weak rectification is expressed in both source and sink phloem tissues of arabidopsis[J].The Plant Cell,2000,12:837-851.

[26] Venekamp JH,Koot JTM.The sources of free proline and aspargine in field plants,Vicia faba L.during and after a short period of water withholding[J].Journal of Plant Physiology,1988,132:102-109.

[27] Voetberg GS,Sharp RE.Growth of the maize primary root at low water potentials1 Ⅲ.Role of increased proline deposition in osmotic adjustment[J].Plant Physiol,1991,96:1 125-1 130.

[28] Bohnert H J,Nelson D E,Jensen R G.Adaptation to environmental stresses[J].The Plant Cell,1995,7:1 099-1 111.

[29] Lutts S,Kinet J M,Bouharmont J.Effects of salt stress on growth,mineral nutrition and proline accumulation in relation to osmotic adjustment in rice (Oryza sativa L.) cultivars differing in salinity resistance[J].Plant Growth Regulation,1996,19(3):207-218.

[30] Moftah A E,Michel B E.The effect of sodium chloride on solute potential and proline accumulation in soybean leaves[J].Plant Physiology,1987,83:238-240.