doi:

DOI: 10.3724/SP.J.1148.2011.00139

Arid Zone Research (干旱区研究) 2011/28:1 PP.139-145

Effect of Drought Stress on Physiological and Biochemical Properties of Amorpha fruticosa Seedlings


Abstract:
In recent years, precipitation has reduced and groundwater has been seriously pumped. Drought trend has become as a global focus. Relevant research, such as the adaptive mechanism and drought resistance of plants to drought habitats, has become as one of the important issues of plant physiological ecology. Amorpha fruticosa is an easily-bred perennial deciduous legume shrub with its strong adaptability to drought stress. Objective of this study is to research the physiological, ecological and adaptive properties of A. fruticosa seedlings under drought stress so as to provide the theory and reference for its utilization and popularization in arid and semiarid areas. Effect of drought stress on physiological and biochemical properties of A. fruticosa were studied with potted seedlings. The changes of net photosynthesis rate (Pn), transpiration rate (Tr), stomatal conductance (Gs), osmotic adjustment, protective enzymes, chlorophyll content, and water status were investigated. Results show that water saturation deficit (WSD), plasma membrane permeability and MDA content of A. fruticosa were increased with the increase of drought stress, while Pn, Tr and Gs were decreased. However, water use efficiency (WUE), SOD activity and contents of K, soluble sugar and free proline (Pro) were increased evidently. Soluble sugar and Pro played an important role in osmotic adjustment. Contents of chlorophyll a (Chla), chlorophyll b (Chlb), total chlorophyll (Chl) and carotenoid (Car) pigment were also increased with the decrease of soil moisture content. Moreover, the change of Chla/Chlb ratio was not so remarkable with the increase of drought stress intensity. A physiological injury of A. fruticosa occurred under the drought stress. Drought stress on physiological and biochemical properties of A. fruticosa seedlings was different to a certian extent. Generally speaking, the higher the drought stress intensity is, the more distinct the index change will be. Results in this study show that Tr, WUE, contents of Pro, soluble sugar and K as well as SOD and POD activity are the objective reflection of the adaptive mechanism of A. fruticosa seedlings to drought habitats and could be used as the identification indexes of its drought resistance. These results reveal that A. fruticosa seedlings increase their drought resistance by changing their physiological properties and have a certain resistance to drought stress. Adaptation of A. fruticosa seedlings could provide the theory and reference for its utilization and popularization in arid and semiarid areas.

Key words:Amorpha fruticosa,drought stress,soil moisture,drought tolerance

ReleaseDate:2014-07-21 15:54:42



[1] 肖春旺,周广胜,马凤云.施水量变化对毛乌素沙地优势植物形态与生长的影响[J].植物生态学报,2002,26(1):69-76.[Xiao Chunwang,Zhou Guansheng,Ma Fengyun.Effect of water supply change on morphology and growth of dominant plants in Maowusu sandland[J].Acta Phytoecologica Sinica,2002,26(1):69-76.]

[2] Lange O L,Kappen L,Schulze E D.Water and Plant Life:Problems and Modern Approaches[M].Berlin:Heidelbergs Springer Verlag,1976.

[3] 王丽华,王静,佘洪军.紫穗槐播种育苗技术[J].林业实用技术,2007(4):46-47.[Wang Lihua,Wang Jing,She Hongjun.Technology of Amorpha fruticosa sowing and seedling raising[J].Practical Forestry Technology,2007(4):46-47.]

[4] 李卫,杨军,郭光沁,等.紫穗槐的离体快速繁殖[J].西北植物学报,2002,22(2):270-277.[Li Wei,Yang Jun,Guo Guangqin,et al.In vitro micro-propagation of Amorpha fruticosa[J].Acta Botanica Boreali-Occidentalia Sinica,2002,22(2):270-277.]

[5] 赵淑梅,张从景.紫穗槐的综合利用及栽培技术[J].防护林科技,2005,66(3):125-126.[Zhao Shumei,Zhang Congjing.Comprehensive utilization and cultivation technique of Amorpha fruticosa[J]. Protection Forest Science and Technology,2005,66(3):125-126.]

[6] 吴志远.地埂柠条和紫穗槐的水保作用与生态及经济效益分析[J].水土保持研究,1999,6(增刊):35-36.[Wu Zhiyuan.Water conservational role of Salix lutea and Amorpha fruticosa planted in field bund and their ecological and economic benefits[J].Research of Soil and Water Conservation,1999,6(Suppl.):35-36.]

[7] 康清海,严国民,金绥庆.黄土高原片沙区建筑梯田生物护埂固沙试验研究[J].水土保持学报,2002,16(5):135-136.[Kang Qinghai,Yan Guomin,Jin Suiqing.Research on biological ridge protection and sand-fixing of building terrace in sheet sand region of Loess Plateau[J].Journal of Soil and Water Conservation,2002,16(5):135-136.]

[8] 张友焱,刘致远,周泽福,等.环境因子对毛乌素沙地紫穗槐光合、蒸腾速率的影响[J].林业科学研究,2006,19(4):463-466.[Zhang Youyan,Liu Zhiyuan,Zhou Zefu,et al.The influence of environment factors on photosynthetic and transpiration rate of Amorpha fruticosa in Maowusu sandland[J].Forest Research,2006,19(4):463-466.]

[9] 杨娜,王冬梅,王百田,等.土壤含水量对紫穗槐蒸腾速率与光合速率影响研究[J].水土保持应用技术,2006(3):6-9.[Yang Na,Wang Dongmei,Wang Baitian,et al.Study on the effect of soil water on Amorpha fruticosa transpiration rate and photosynthetic rate[J].Technology of Soil and Water Conservation,2006(3):6-9.]

[10] 曹艳萍,卢翠英,白根举.紫穗槐叶中杀虫化学成分的分离和结构鉴定[J].化学研究与应用,2004,16(5):719-720.[Cao Yanping,Lu Cuiying,Bai Genju.Separation and identification of pesticide components of Amorpha fruticosa[J].Chemical Research and Application,2004,16(5):719-720.]

[11] 刘畅,姜泓,张建逵,等.GC-MS法测定紫穗槐果实挥发油中的化学成分[J].中华中医药学刊,2008,26(1):213-214.[Liu Chang,Jiang Hong,Zhang Jiankui,et al.Analysis of essential oil in dry fruits of Amorpha fruticosa by GC-MS[J].Chinese Archives of Traditional Chinese Medicine,2008,26(1):213-214.]

[12] 高桂枝,苑姗姗,虞梅.紫穗槐协同杀虫成分分离及其毒力研究[J].现代农药, 2008, 7(5):43-45.[Gao Guizhi,Yuan Shanshan,Yu Mei.Studies on separation of compounds and toxicity of the insecticide in Amorpha fruticosa [J]. Modern Agrochemicals,2008,7(5):43-45.]

[13] 李文华,刘广权,马松涛,等.干旱胁迫对苗木蒸腾耗水和生长的影响[J].西北农林科技大学学报: 自然科学报,2004,32(1):61-65.[Li Wenhua,Liu Guangquan,Ma Songtao,et al.Effect of drought stress on transpiration and growth characteristics of young plant[J].Journal of Northwest Agriculture and Forest University: Natural Science Edition,2004,32(1):61-65.]

[14] 王得祥,康博文,刘建军,等.主要城市绿化树种苗木耗水特性研究[J].西北林学院学报,2004,19(4):20-23.[Wang Dexiang,Kang Bowen,Liu Jianjun,at al.Water consumption traits of several city greening seedlings[J].Journal of Northwest Forestry University,2004,19(4):20-23.]

[15] 赵世杰,许长成,邹琦,等.植物组织中丙二醛测定方法的改进[J].植物生理学通讯,1994,30(3):207-210.[Zhao Shijie,Xu Changcheng,Zou Qi,et al.Improvements of method for measurement of malondialdehyde in plant issues[J].Plant Physiology Communications,1994,30(3):207-210.]

[16] 邹绮.植物生理学实验指导[M].北京: 中国农业出版社, 2000.[Zou Qi.Experimental Guide of Plant Physiology[M].Beijing:China Agricultural Press,2000.]

[17] 丛日春,胡雅君,刘洪庆.几种攀缘植物耐旱性研究[J].内蒙古林学院学报, 1996,18(3):33-39.[Cong Richun,Hu Yajun,Liu Hongqing.Research on drought resistance of several climbing plants[J].Journal of Neimonggol Forestry College,1996,18(3):33-39.]

[18] 张建国,李吉跃,沈国舫.树木耐旱特征及其机理研究[M].北京:中国林业出版社,2000:45-61.[Zhang Jianguo,Li Jiyue,Shen Guofang.Studies on Drought Tolerant Characteristics and Mechanism Research of Trees[M].Beijing:China Forestry Press,2000:45-61.]

[19] 时连辉,牟志美,姚健.不同桑树品种在土壤水分胁迫下膜伤害和保护酶活性变化[J].蚕业科学, 2005,31(4):13-17.[Shi Lianhui,Mu Zhimei,Yao Jian.Cell membrane damage and change of protective enzymes’activity in different mulberry varieties under soil water stress[J].Science of Sericulture,2005,31(4):13-17.]

[20] 史吉平,董永华.水分胁迫对小麦光合作用的影响[J].麦类作物,1995(5):49-51.[Shi Jiping,Dong Yonghua.The effect of water stresses to photosynthesis of wheat[J].Journal of Triticeae Crops,1995(5):49-51.]

[21] 黄建昌.草毒对干旱的生理反应[J].果树科学,1994,11(2):114-116.[Huang Jianchang.Physiological reaction of strawberry under rapid drought stress[J].Journal of Fruit Science,1994,11(2):114-116.]

[22] 王晶英,赵雨森,王臻,等.干旱胁迫对银中杨生理生化特性的影响[J].水土保持学报,2006,20(1):197-200.[Wang Jingying,Zhao Yusen,Wang Zhen,et al.Effect of drought stress on physiologic and biochemical characteristic of Populus alba×Populus berolinensis [J]. Journal of Soil and Water Conservation,2006,20(1):197-200.]

[23] 任红旭,陈雄,吴冬秀. CO2浓度升高对干早胁迫下蚕豆光合作用和抗氧化能力的影响[J].作物学报,2001,27(6):11-18.[Ren Hongxu,Chen Xiong,Wu Dongxiu.Effects of elevated CO2 on photosynthesis and antioxidative ability of broad bean plants grown under drought condition[J].Acta Agronomica Sinica,2001,27(6):11-18.]

[24] 华春,周泉澄,张边江,等.毕氏海蓬子和盐角草幼苗对PEG 6000模拟干旱的生理响应[J].干旱区研究,2009,26(5):702-707.[Hua Chun,Zhou Quancheng,Zhang Bianjiang,et al.Physiologic response of seedlings of Salicornia bigelovii Torr. and Salicornia europaea L. to drought simulated with PEG 6000[J]. Arid Zone Reseach,2009,26(5):702-707.]

[25] 张成军.辽东栎林中四种木本植物幼苗对土壤干旱的生理生态响应[D].哈尔滨:东北林业大学博士论文,2003.[Zhang Chengjun.Ecophysiologcial Responses to Soil Drought in Four Woody Species Seedling in Quercus Liaotungensis Forest[D].Harbin:Northeast Forestry University,2003.]

[26] 阎勇,罗兴录,张兴思,等.不同供水条件下玉米耐旱生理特性比较[J].中国农学通报,2007,23(9):323-326.[Yan Yong,Luo Xinglu,Zhang Xingsi,et al.Comparison of maize physiological characters under different treatment of irrigation[J].Chinese Agricultural Science Bulletin,2007,23(9):323-326.]

[27] Park K J,Choi M H,Hong J H.Control of composting odor using biofiltration[J].Compost Science and Utilization,2002,10(4):356-362.

[28] 柴胜丰,蒋运生,韦霄,等.干旱胁迫对槐树幼苗生长和生理生态特征的影响[J].浙江林业科技,2008,28(2):43-47.[Chai Shengfeng,Jiang Yunsheng,Wei Xiao,et al.Effect of drought stress on growth and eco-physiological properties of Sophora japonica seedlings[J].Journal of Zhejiang Forestry Science and Technology,2008,28(2):43-47.]

[29] 陈立松,刘星辉.水分胁迫对荔枝叶片活性氧代谢的影响[J].园艺学报,1998,25(3):241-246.[Chen Lisong,Liu Xinghui.Effects of water stress on active oxygen metabolism in Litchi leaves[J].Acta Horticulturae Sinica,1998,25(3):241-246.]

[30] 刘涛,李柱,安沙舟,等.干旱胁迫对木地肤幼苗生理生化特性的影响[J].干旱区研究,2008,25(2):231-235.[Liu Tao,Li Zhu,An Shazhou,et al.Effects of drought stress on physiological and biochem ical properties of Kochia prostrata (L.) schrad seedlings[J].Arid Zone Research,2008,25(2):231-235.]

[31] 石喜,王密侠,姚雅琴,等.水分亏缺对玉米植株干物质累积、水分利用效率及生理指标的影响[J].干旱区研究,2009,26(3):396-400.[Shi Xi,Wang Mixia,Yao Yaqin,et al.Effects of water deficit on dry matter accumulation,WUE and physiological indexes of Maize [J].Arid Zone Research,2009,26(3):396-400.]

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