doi:

DOI: 10.3724/SP.J.1245.2012.00280

Asian Herpetological Research 2012/3:4 PP.280-287

Impact of the Qinghai-Tibet Railway on Population Genetic Structure of the Toad-Headed Lizard, Phrynocephalus vlangalii


Abstract:
Using data from nine microsatellite DNA loci and a population genetic approach, we evaluate the barrier effect of the Qinghai-Tibet Railway on toad-headed lizard, Phrynocephalus vlangalii. The study area is along a 20 km stretch of the railway on northern Qinghai-Tibet Plateau, and this section of the railway was constructed between 1958–1979. Both assignment tests and analysis of molecular variance (AMOVA) were used for data analysis. We found significant genetic differentiation between the populations from the study area and those from a further southeastern area, which are separated by a 20 km gap. This suggests the existence of population substructure at a fine-scale. However, we did not detect any difference between samples from the western and eastern sides of the railway within the study area, and concluded that the railway may not impose a significant barrier effect on these lizard populations at the present time. Available suitable habitat alongside the railway and bridge underpasses may have facilitated the gene exchange between the sides. The relatively short time since the completion of the railway may not allow the differentiation to accumulate to a detectable level. Since the Qinghai-Tibet Plateau maintains a unique and fragile ecosystem, long-term monitoring of such man-made landscape features is imperative for protecting this ecosystem.

Key words:Qinghai-Tibet Railway, barrier effect, population structure, Phrynocephalus vlangalii, microsatellite DNA, Bayesian assignment test

ReleaseDate:2014-07-21 16:32:25



Andrews K. M., Gibbons J. W. 2005. How do highways influence snake movement? Behavioral responses to roads and vehicles. Copeia, 2005: 772-782

Bartoszek J., Greenwald K. R. 2009. A population divided: Railroad tracks as barriers to gene flow in an isolated population of marbled salamanders (Ambystoma opacum). Herpetol Conserv Biol, 4: 191-197

Berry O., Gleeson D. M., Sarre S. D. 2003. Microsatellite DNA markers for New Zealand skinks. Conserv Genet, 4: 411-414

Crosby M. K. A., Licht L. E., Fu J. 2009. The effect of habitat fragmentation on finescale population structure of wood frog (Rana sylvatica). Conserv Gen, 10: 1707-1718

DiLeo M. F., Row J. R., Lougheed S. C. 2010. Discordant patterns of population structure for two co-distributed snake species across a fragmented Ontario landscape. Diver Distrib, 16: 571-581

Epps C. W., Palsboll P. J., Wehausen J. D., Roderick G. K., Ramey II R. R., McCullough D. R. 2005. Highways block gene flow and cause a rapid decline in genetic diversity of desert bighorn sheep. Ecol Lett, 8: 1029-1038

Ernst J. A., Branch L. C., Clark A. M., Hokit D. G. 2004. Polymorphic microsatellite markers for the Florida scrub lizard (Sceloporus woodi). Mol Ecol Notes, 4: 364-365

Excoffier L., Lischer H. 2011. Arlequin (version 3.5): An integrated software package for population genetic data analysis. User Manual distributed by the authors

Fahrig L., Rytwinski T. 2009. Effects of roads on animal abundance: An empirical review and synthesis. Ecol Soc, 14: 21

Forman R. T. T., Alexander L. E. 1998. Roads and their major ecological effects. Ann Rev Ecol Syst, 29: 207-231

Gibbs J. P., Shriver W. G. 2005. Can road mortality limit populations of pool-breeding amphibians? Wetlands Ecol Manag, 13: 281-289

Lesbarrères D., Primmer C. R., Lodé T., Merilä J. 2006. The effects of 20 years of highway presence on the genetic structure of Rana dalmatina populations. Ecoscience, 13: 531-538

Moore J. A., Miller H. C., Daugherty C. H., Nelson N. J. 2008. Fine-scale genetic structure of a long-lived reptile reflects recent habitat modification. Mol Ecol, 17: 4630-4641

Manel S., Schwartz M. K., Luikart G., Taberlet P. 2003. Landscape genetics, combining landscape ecology and population genetics. Trends Ecol Evol, 18: 189-197

Paetkau D., Slade R., Burden M., Estoup A. 2004. Genetic assignment methods for the direct, real-time estimation of migration rate: A simulation based exploration of accuracy and power. Mol Ecol, 13: 55-65

Piry S., Alapetite A., Cornuet J. M., Paetkau D., Baudouin L., Estoup A. 2004. GeneClass2: A software for genetic assignment and first-generation migrant detection. J Hered, 95: 536-539

Pritchard J. K., Stephans M., Donnelly P. 2000. Inference of population structure using multilocus genotype data. Genetics, 155: 945-959

Pritchard J. K., Wen X., Falush D. 2009. Documentation for structure software: Ver. 2.3. Available from http://pritch.bsd. uchicago.edu/structure.html

Qi Y., Li S. S., Suo L., Li H., Wang Y. Z. 2011. An ethogram of the toad-headed lizard Phrynocephalus vlangalii during the breeding season. Asian Herpetol Res, 2(2): 110-116

Rannala B., Mountain J. L. 1997. Detecting immigration by using multilocus genotypes. Proc Natl Acad Sci USA, 94: 9197-9201

Raymond M., Rousset F. 1995. Genepop (ver. 1.2): Population genetic software for exact tests and ecumenicism. J Hered, 86: 248-249

Riley S. P. D., Pollinger J. P., Sauvajot R. M., York E. C., Bromley C., Fuller T. K., Wayne R. K. 2006. A southern California freeway is a physical and social barrier to gene flow in carnivores. Mol Ecol, 15: 1733-1741

Rousset F. 2008. Genepop’007: A complete reimplementation of the genepop software for Windows and Linux. Mol Ecol Res, 8: 103-106

Shine R., Lemaster M., Wall M., Langkilde T., Mason R. 2004. Why did the snake cross the road? Effects of roads on movement and location of mates by garter snakes (Thamnophis sirtalis parietalis). Ecol Soc, 9: 9

Trombulak S. C., Frissell C. A. 2000. Review of the ecological effects of roads on terrestrial and aquatic ecosystems. Conserv Biol, 14: 18-30

Urquhart J. W., Bi K., Gozdzik A., Fu J. 2005. Isolation and characterization of microsatellite DNA loci in the toad-headed lizards, Phrynocephalus przewalskii complex. Mol Ecol Notes, 5: 928-930

Wang Y., Zhan A., Fu J. 2009. Testing historical phylogeographic inferences with contemporary gene flow data: Population genetic structure of the Qinghai toad-headed lizard. J Zool, 278: 149-156

Waser P. M., Hadfield J. D. 2011. How much can parentage analyses tell us about precapture dispersal? Mol Ecol, 20: 1277-1288

Xia L., Yang Q., Li Z., Wu Y., Feng Z. 2007. The effect of the Qinghai-Tibet railway on the migration of Tibetan antelope Pantholops hodgsonii in Hoh-xil National Nature Reserve, China. Oryx, 41: 352-357

Yanes M., Velasco J. M., Suárez F. 1995. Permeability of roads and railways to vertebrates: The importance of culverts. Biol Conserv, 71: 217-222

Yang Q., Xia L. 2008. Tibetan wildlife is getting used to the railway. Nature, 452: 810-811

Yin B., Huai H., Zhang Y., Zhou L., Wei W. 2006. Influence of the Qinghai-Tibetan railway and highway on the activities of wild animals. Acta Ecol Sin, 26: 3917-3923

Zhan A., Fu J. 2009. Microsatellite DNA markers for three toadheaded lizard species (Phrynocephalus vlangalii, P. przewalskii and P. guttatus). Mol Ecol Res, 9: 535-538

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