DOI: 10.3724/SP.J.1245.2014.00240

Asian Herpetological Research 2014/5:4 PP.240-244

Group-spawning and Simultanous Polyandry of a Stream-dwelling Frog Feirana kangxianensis

Here we provide an example of simultaneous polyandry based on genetic evidence in Feirana kangxianensis. This stream-dwelling species occurs only in Kangxian County, southern Gansu Province, where it is sympatric with its sibling species F. quadranus. During the breeding season the sex ratio of F. kangxianensis was marginally femalebiased(44♂:59♀) and the encounter rate in a relatively pristine habitat was significantly higher than that in heavily quarried habitats(9.6 ± 4.8 indiv./km vs. 3.2 ± 2.5 indiv./km). Three egg masses containing an average of 698 eggs were deposited on the underside of one or two adjacent flat rocks, 6.0-10.0 cm under the water surface and 1.0-3.5 cm above the streambed. Using Bayesian sibship clustering of nine polymorphic microsatellite genotypes, two females were detected as group-spawning in one oviposition site, with three males fertilizing each female's eggs simultaneously. We also discuss the conservation requirements of this range-restricted species and the evolutionary implication of its unusual reproductive strategy.

Key words:group-spawning,oviposition site,sex ratio,simultaneous polyandry,habitat conservation

ReleaseDate:2015-03-27 09:32:50

Byrne P. G., Roberts J. D. 2012. Evolutionary causes and consequences of sequential polyandry in anuran amphibians. Biol Rev, 87, 209-228

Chen X. H., Yang J., Qiao L., Zhang L. X., Lu X. 2011. Reproductive ecology of the stream-dwelling frog Feirana taihangnicus in central China. Herpetol J, 21, 135-140

Fei L., Ye C. Y., Jiang J. P. 2012. Colored Atlas of Chinese Amphibians and Their Distributions. Chengdu: Sichuan Publishing House of Sciences and Technology

Gonser R. A., Collura R. V. 1996. Waste not, want not: toe-clips as a source of DNA. J Herpetol, 30, 445-447

Huelsenbeck J. P., Ronquist F. 2001. MrBayes: Bayesian inference of phylogeny. Bioinformatics, 17, 754-755

Jones O. R., Wang J. 2010. COLONY: a program for parentage and sibship inference from multilocus genotype data. Mol Ecol Resour, 10, 551-555

Kalinowski S. T., Taper M. L., Marshall T. C. 2007. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol, 16, 1099-1106

Marshall, T. C., Slate J., Kruuk L. E. B., Pemberton J. M. 1998. Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol, 7, 639-655

Roberts J. D., Byrne P. G. 2011. Polyandry sperm competition, and the evolution of anuran amphibians. Adv Study Behav, 43, 1-53

Ronquist F., Teslenko M., van der Mark P., Ayres D. L., Darling A., Höhna S., Larget B., Liu L., Suchard M. A., Huelsenbeck J. P. 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol, 61, 539-542

Seymour R. S., Bradford D. F. 1995. Respiration of amphibian eggs. Physiol Zool, 68, 1-25

Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol, 30, 2725-2729

Tao J., Yang J., Chen X. H. 2010. The early embryonic development and ecological adaptation of Feirana taihangnicus. Chin J Zool, 45, 39-46

Wang B., Jiang J. P., Xie F., Chen X. H., Dubois A., Liang G., Wagner S. 2009. Molecular phylogeny and genetic identification of populations of two species of Feirana Frogs (Amphibia: Anura, Ranidae, Dicroglossinae, Paini) endemic to China. Zool Sci, 26, 500-509

Wang J., Zhang J., Yang X., Li X. L., Jiang J. P. 2014. Isolation and characterization of 26 microsatellite loci for the frog Feirana quadranus. Conserv Genet Resour, 6, 57-58

Wells K.D. 2007. The Ecology and Behavior of Amphibians. Chicago: University of Chicago Press

Yang X., Wang B., Hu J. H., Jiang J. P. 2011. A new species of the Genus Feirana (Amphibia: Anura: Dicroglossidae) from the western Qinling Mountains of China. Asian Herpetol Res, 2, 72-86

Zhang L. X., Yang J., Lu Y., Lu X., Cheng X. H. 2012. Aquatic eggs are fertilized by multiple males not engaged in amplexus in a stream-breeding frog. Behav Processes, 91, 304-307