DOI: 10.3724/SP.J.1245.2014.00281

Asian Herpetological Research 2014/5:4 PP.281-283

Development and Characterization of Fifteen Polymorphic M icosatellites for Extremely Endangered Salamander, Echinotriton chinhaiensis

Echinotriton chinhaiensis is a critically endangered salamander and its distribution is restricted to Ningbo, Zhejiang Province of China. In this study, we developed and characterized fifteen polymorphic microsatellite loci for E. chinhaiensis from dinucleotide- and tetranucleotide- enriched library. The number of alleles ranges from 4 to 12 with an average of 7.27 alleles per locus. The observed and expected heterozygosities values were from 0.250 to 0.844 and 0.511 to 0.872 with an average value of 0.596 and 0.722; respectively. The polymorphic microsatellite loci described in this paper are useful in the further study on genetic diversity and gene flow, which would be helpful to formulate effective conservation strategies for the E. chinhaiensis.

Key words:Echinotriton chinhaiensis,polymorphic microsatellite loci,population genetics,conservation biology

ReleaseDate:2015-03-27 09:32:51

Houlahan J. E., Findlay C. S., Schmidt B. R., Meyer A. H., Kuzmin S. L. 2000. Quantitative evidence for global amphibian population declines. Nature, 404: 752-755

Liu C, Xie F, Jiang JP, Zheng ZH, Liu XT, Liu Y, Wu MF. 2010. Annual reproduction comparision and analysis of Chinhai Salamander (Echinotriton chinhaiensis) Ruiyansi Population. Sichuan Journal of Zoology Vol. 29 No. 1: 24-30.

Stuart SN, Chanson JS, Cox NA, Young BE, Rodrigues ASL, Fischman DL, Waller RW. 2004. Status and trends of amphibian declines and extinctions worldwide. Science, 306: 1783-1786

Zane L, Bargelloni L, Patarnello T. 2002. Strategies for microsatellite isolation: a review. Molecular Ecology, 11, 1-16