DOI: 10.3724/SP.J.1003.2012.06012

Biodiversity Science (生物多样性) 2012/20:3 PP.324-329

Convergence in host recognition behavior between obligate pollinating fig wasps and non-pollinating fig wasps

The highly specific mutualism between fig trees and their obligate pollinating fig wasps is usually exploited by non-agaonid wasps, and some of these wasps can enter and pollinate the figs just like the obligate pollinating wasps. Therefore, the agaonid and non-agaonid wasps have convergently evolved in their morphological characteristics and phenology. However, there are few data about the convergence of host recognition behaviors among these wasps. In Ficus curtipes, there are three internally ovipositing wasps, i.e. one obligate pollinating wasp, Eupristina sp., and two inquiline wasps (Diaziella yangi and Lipothymus sp.), which can also pollinate the figs if they enter the figs. In this study, we carried out several behavioral ex-periments with a Y-tube olfactometer to test the hypothesis of convergence of host recognition behaviors among these wasps. We observed and recorded the wasps’ behavior of choice among figs at different devel-opmental phases and among 6-methyl-5-hepten-2-ol, 6-methyl-5-hepten-2-one and the mixture of the two chemicals. Our results showed that all three of the wasps were significantly attracted by the receptive F. cur-tipes figs when presented with choices between receptive figs and figs at other developmental phases and were significantly repelled by the male phase figs of F. curtipes when presented with choices between male phase figs and figs at other developmental phases. In addition, all of them also preferred to the compound 6-methyl-5-hepten-2-ol at the dose level of 1µL. These results provide evidence for the hypothesis of con-vergence of host recognition behavior among obligate pollinating fig wasps and non-pollinating fig wasps. The role of behavioral convergence in the evolution of non-obligate pollinating wasps into obligate ones is also discussed.

Key words:Ficus, Agaonidae, Ficus curtipes, host recognition, behavioral convergence, obligate pollination system

ReleaseDate:2014-07-21 16:28:15

Anstett MC, Hossaert-McKey M, Kjellberg F (1997) Figs and fig pollinators: evolutionary conflicts in a coevolved mutualism. Trends in Ecology and Evolution, 12, 94-99.

Chen C, Song QS, Proffit M, Bessière JM, Li ZB, Hos-saert-McKey M (2009) Private channel: a single unusual compound assures specific pollinator attraction in Ficus semicordata. Functional Ecology, 23, 941-950.

Compton SG, Grehan K, van Noort S (2009) A fig crop polli-nated by three or more species of agaonid fig wasps. African Entomology, 17, 215-222.

Cook JM, Segar ST (2010) Speciation in fig wasps. Ecological Entomology, 35(Suppl.1), 54-66.

Galil J, Eisikowitch D (1968) On the pollination ecology of Ficus sycomorus in East Africa. Ecology, 49, 259-269.

Harrison RD (2005) Figs and the diversity of tropical rainfor-ests. BioScience, 55, 1053-1064.

Herre EA, Jandér K, Machado CA (2008) Evolutionary ecol-ogy of figs and their associates: recent progress and out-standing puzzles. Annual Review of Ecology, Evolution, and Systematics, 39, 439-458.

Janzen DH (1979) How to be a fig. Annual Review of Ecology and Systematics, 10, 13-51.

Jousselin E, Rasplus JY, Kjellberg F (2001) Shift to mutualism in parasitic lineages of the fig/fig wasp interaction. Oikos, 94, 287-294.

Kerdelhué C, Rossi JP, Rasplus JY (2000) Comparative com-munity ecology studies on Old World figs and fig wasps. Ecology, 81, 2832-2849.

Khadari B, Gibernau M, Anstett MC, Kjellberg F, Hos-saert-McKey M (1995) When figs wait for pollinators: the length of fig receptivity. American Journal of Botany, 82, 992-999.

Kobmoo N, Hossaert-McKey M, Rasplus JY, Kjellberg F (2010) Ficus racemosa is pollinated by a single population of a single agaonid wasp species in continental South-East Asia. Molecular Ecology, 19, 2700-2712.

Michaloud G, Carrière S, Kobbi M (1996) Exceptions to the one:one relationship between African fig trees and their fig wasp pollinators: possible evolutionary scenarios. Journal of Biogeography, 23, 513-520.

Molbo D, Machado CA, Sevenster JG, Keller L, Herre EA (2003) Cryptic species of fig-pollinating wasps: implications for the evolution of the fig-wasp mutualism, sex allocation, and precision of adaptation. Proceedings of the National Academy of Sciences, USA, 100, 5867-5872.

Proffit M, Schatz B, Bessière JM, Chen C, Soler C, Hos-saert-McKey M (2008) Signalling receptivity: comparison of the emission of volatile compounds by figs of Ficus his-pida before, during and after the phase of receptivity to pol-linators. Symbiosis, 45, 15-24.

Su ZH, Iino H, Nakamura K, Serrato A, Oyama K (2008) Breakdown of the one-to-one rule in Mexican fig-wasp as-sociations inferred by molecular phylogenetic analysis. Symbiosis, 45, 73-81.

van Noort S, Compton SG (1996) Convergent evolution of agaonine and sycoecine (Agaonidae, Chalcidoidea) head shape in response to the constraints of host fig morphology. Journal of Biogeography, 23, 415-424.

Weiblen GD (2002) How to be a fig wasp. Annual Review of Entomology, 47, 299-330.

Yang P, Li ZB, Peng YQ, Yang DR (2012) Exchange of hosts: can agaonid fig wasps reproduce successfully in the figs of non-host Ficus? Naturwissenschaften, 99, 199-205.

Zhang FP, Peng YQ, Guan JM, Yang DR (2008) A species of fig tree and three unrelated fig wasp pollinators. Evolution-ary Ecology Research, 10, 611-620.

Zhang FP (张凤萍) (2009) Shift to Mutualism in Parasitic Lineages of the Fig-Wasp Interaction (非传粉榕小蜂由寄生向互惠演化的机制). PhD dissertation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming. (in Chinese with English abstract)

Zhang FP (张凤萍), Peng YQ (彭艳琼), Yang DR (杨大荣) (2008) Coevolution between two internal ovipositing fig wasps and host Ficus curtipes. Journal of Plant Ecology (植物生态学报), 32, 768-775. (in Chinese with English ab-stract)

Zhang FP (张凤萍), Peng YQ (彭艳琼), Yang DR (杨大荣) (2009) The comparison of three fig wasp pollinators and their pollination efficiency on Ficus curtipes. Acta Ecologica Sinica (生态学报), 29, 5252-5257. (in Chinese with English abstract)