doi:

DOI: 10.3724/SP.J.1259.2015.00338

Chinese Bulletin of Botany (植物学报) 2015/50:3 PP.338-345

Identification of Cold Tolerance at the Plumule Stage Quantitative Trait Loci with Single Segment Substituted Lines in Rice


Abstract:
Cold tolerance at the plumule stage is an important trait during rice growth and can be easily affected by genetic background and environmental factors. Single segment substitution lines (SSSLs) can effectively reduce the inter-ference of the genetic background. We developed a set of genome-wide SSSLs carrying Nipponbare introgression segments in the background of Guangluai 4. Quantitative trait loci (QTL) analysis of cold tolerance at the plumule stage involved one-way ANOVA and Dunnett's test for comparing SSSLs with the recurrent parent Guangluai 4 (P ≤ 0.001). We detected eight QTL for cold tolerance at the plumule stage on chromosomes 1, 6, 8, 9 and 10; all had a positive effect. In both years studied, the additive effect of these QTL ranged from 14% to 44% and 10% to 45%, with additive effect percentages 700% to 2 200% and 500% to 2 250%. Four of these QTL were primarily mapped by using the substitution mapping method, and qCTP9-2 had the largest additive effect in both years for 44% and 45%, respectively. These results provide useful information for exploring and using new grain number-related resources.

Key words:rice;plumule stage;cold tolerance;quantitative trait loci;single segment substitution lines;substitution mapping

ReleaseDate:2015-07-13 09:55:51



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