doi:

DOI: 10.3724/SP.J.1259.2015.00346

Chinese Bulletin of Botany (植物学报) 2015/50:3 PP.346-353

The Effects of 2E, 4E-decadienal on the Growth and Photosynthesis of Three Species of Phytoplankton


Abstract:
Polyunsaturated aldehydes (PUAs) are cleaved from fatty acid precursors by enzymes activated within seconds of diatom cell wounding. These aldehydes are responsible for a suite of physiological dysfunctions during egg development, hatching and morphogenesis in larvae of copepods, therefore relieving the grazing risks. However, few studies have investigated the allelopathic effect of PUAs on phytoplankton. We explored whether the production of reactive volatile aldehydes can be linked to their ability to cause growth and photosynthesis failure in phytoplankton. Prorocentrum donghaiense, Chaetoceros didymus and Prymnesium parvum were exposed to 2E, 4E-decadienal (DEC) at different concentrations, and the growth and photosynthesis were monitored frequently. We did not find any negative effect of DEC at 0.5 mg·L-1 on the growth and photosynthesis of phytoplankton. However, DEC at 1 mg·L-1 inhibited the cell abundance and Fv/Fm of P. donghaiense but had no effect on C. didymus and P. parvum. The growth and photosynthesis of all three selected phytoplanktons were depressed significantly with DEC at 5 mg·L-1, whereas cell density, Fv/Fm, yield and rETR decreased to 0 with PUAs at 10 mg·L-1. PUAs clearly inhibited the growth and photosynthesis of phytoplankton. However, the response of phytoplankton to PUAs is species-specific. Diatom-derived PUAs can suppress the growth of competitive phytoplankton under nutrition-limited conditions, thereby supporting the biomass accumulation of diatoms.

Key words:2E;4E-decadienal;growth inhibition;PUAs;photosynthesis;phytoplankton

ReleaseDate:2015-07-13 09:55:52



Ban S, Burns C, Castel J, Chaudron Y, Christou E, Es-cribano R, Fonda Umani S, Gasparini S, Guerrero Ruiz F, Hoffmeyer M, Ianora A, Kang HK, Laabir M, Lacoste A, Miralto A, Ning XR, Poulet S, Rodriguez V, Runge J, Shi JX, Starr M, Uye SI, Wang YJ (1997). The paradox of diatom-copepod interactions. Mar Ecol Prog Ser 157, 287-293.

Casotti R, Mazza S, Brunet C, Vantrepotte V, Ianora A, Miralto A (2005). Growth inhibition and toxicity of the algal aldehgde 2-trans-2-cis decadienal on Thalassiosira weissflogii (Bacillariophyceae). J Phycol 41, 7-20.

Field CB, Behrenfeld MJ, Randerson JT, Falkowski P (1998). Primary production of the biosphere:integrating terrestrial and oceanic components. Science 281, 237-240.

Hay ME, Kubanek J (2002). Community and ecosystem level consequences of chemical cues in the plankton. J Chem Ecol 28, 2001-2016.

Ianora A, Miralto A (2010). Toxigenic effects of diatoms on grazers, phytoplankton and other microbes:a review. Ecotoxicology 19, 493-511.

Kolber Z, Zehr J, Falkowski P (1988). Effects of growth irradiance and nitrogen limitation on photosynthetic energy conversion in photosystem Ⅱ. Plant Physiol 88, 923-929.

Kromkamp JC, Forster RM (2003). The use of variable fluorescence measurements in aquatic ecosystems:differences between multiple and single turnover measuring protocols and suggested terminology. Eur J Phycol 38, 103-112.

Miralto A, Barone G, Romano G, Poulet SA, Ianora A, Russo GL, Buttino I, Mazzarella G, Laabir M, Cabrini M, Giacobbe MG (1999). Giacobbe MG. The insidious effect of diatoms on copepod reproduction. Nature 402, 173-176.

Pohnert G (2000). Wound-activated chemical defense in unicellular planktonic algae. Angew Chem Int Ed 39, 4352-4354.

Pohnert G, Lumineau O, Cueff A, Adolph S, Cordevant C, Lange M, Poulet S (2002). Are volatile unsaturated al-dehydes from diatoms the main line of chemical defence against copepods? Mar Ecol Prog Ser 245, 33-45.

Ribalet F, Berges JA, Ianora A, Casotti R (2007a). Growth inhibition of cultured marine phytoplankton by toxic al-gal-derived polyunsaturated aldehydes. Aquatic Toxicol 85, 219-227.

Ribalet F, Wichard T, Pohnert G, Ianora A, Miralto A, Casotti R (2007b). Age and nutrient limitation enhance polyunsaturated aldehyde production in marine diatom. Phytochemistry 68, 2059-2607.

Ribalet F, Vidoudez C, Cassin D, Pohnert G, Ianora A, Miralto A, Casotti R (2009). High plasticity in the pro-duction of diatom-derived polyunsaturated aldehydes under nutrient limitation:physiological and ecological implications. Protist 160, 444-451.

Wichard T, Poulet SA, Pohnert G (2005). Determination and quantification of α,β,γ,δ-unsaturated aldehydes as pentafluorobenzyl-oxime derivates in diatom cultures and natural phytoplankton populations:application in marine field studies. J Chromatogr B 814, 155-161.