Chinese Bulletin of Botany (植物学报) 2015/50:3 PP.378-387
Unfertilized ovary or ovule culture is an effective technology for producing homozygous materials (doubled haploids) via gynogenesis. We studied the effects of basal medium and bud developmental stage on the genogenesis in seven lily genotypes, including one wild species and six cultivars. Gynogenesis occurred in all genotypes, but BDS was the optimal basal medium as compared with CBM and MS. When ovary sections from buds collected 1 d before anthesis were cultured in BDS medium, more embryogenic ovules or responding ovules were obtained. To estimate the effect of phytohormones on lily gynogenic embryogenesis, two genotypes were tested. An amount of 2 mg·L-1 2,4-dichloro- phenoxyacetic acid (2,4-D) and 2 mg·L-1 6-benzylaminopurine (6-BA) or kinetin (KT) was suitable for embryo induction from unfertilized ovaries. During culture, embryo development was observed by the cleaning-squash technique; most ovules contained one embryo, which lay on the micropylar end, zygote end or polar nucleus position. In addition, a few ovules with two embryos were observed. In total, 146 regenerants were obtained from unpollinated ovary culture of five genotypes; for 62, the ploidy level was determined by chromosome counting. We found that 43 plantlets had a chromosome number different from maternal plants.
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