doi:

DOI: 10.3724/SP.J.1096.2013.30494

Chinese Journal of Analytical Chemistry (分析化学) 2013/41:12 PP.1812-1816

Establishment of a Method Combined Immunomagnetic Separation with Colloidal Gold Lateral Flow Assay and Its Application in Rapid Detection of Escherichia. coli O157:H7


Abstract:
The immunomagnetic beads were prepared by coupling anti-Escherichia. coli O157:H7 monoclonal antibody with magnetic beads. The colloidal gold test strip was composited with anti-O157:H7 monoclonal antibody marked by colloidal gold as the detection antibody, anti-E.coli O157:H7 polyclonal antibody as the test line and the donkey anti-mouse IgG secondary antibody as the control line. Immunomagnetic separation was combined with colloidal gold lateral flow assay, and this method was applied in the rapid detection of E.coli O157:H7. The results showed that 1 mL of 7.6×103 CFU/mL of E.coli O157:H7 gave a positive result by using colloidal gold lateral flow after immunomagnetic separation. Food sample spiked with 10 CFU/g of E.coli O157:H7 after 6 h of enrichment could be detected within 72 min of immunomagnetic separation and colloidal gold lateral flow assay. The method is of great value to the rapid on-site detection of harmful substance in food.

Key words:Immunomagnetic separation,Colloidal gold,Immunochromatography,Escherichia. coli O157:H7

ReleaseDate:2015-04-19 11:07:53



牛 牧, 杜美红, 邓 奕, 高明远. 高等学校化学学报, 2011, 32(2): 322-326

1 NIU Mu, DU Mei-Hong, DENG Yi, GAO Ming-Yuan. Chem. J. Chinese Universities, 2011, 32(2): 322-326

颜小飞, 汪懋华, 温新华, 安 冬. 分析化学, 2013, 41(6): 817-821

2 YAN Xiao-Fei, WANG Mao-Hua, WEN Xin-Hua, AN Dong. Chinese J. Anal. Chem., 2013, 41(6): 817-821

3 Sung Y J, Suk H J, Sung H Y, Li T, Poo H, Kim M. Biosensors and Bioelectronics, 2013, 43: 432-439

李智洋, 何 磊, 何农跃, 史智扬, 汪 华, 李 松, 刘洪娜, 戴亚斌. 化学学报, 2010, 68(03): 251-256

黄岭芳, 段 霞, 陈 媛, 刘文娟, 魏 华, 赖卫华. 食品科学, 2010, 31(24): 355-359

4 Yoshitomi K J, Jinneman K C, Zapata R, Weagant S D, Fedio W M. Journal of Food Science, 2012, 77(8): M481-M489

5 Mendonça M, Conrad N L, Conceição F R, MoreiraÅ N, da Silva W P, Aleixo, J A, Bhunia A K. BMC Microbiology, 2012, 12(1): 275

6 Leuvering J H, Thal P J, Van Der Waart M, Schuurs A H. Journal of Immunological Methods, 1981, 45(2): 183-194

7 Le T, Yan P, Xu J, Hao Y. Food Chemistry, 2013, 138(2-3): 1610-1615

8 Moon J, Kim G, Lee S. Materials, 2012, 5(4): 634-643

9 Ferens W A, Hovde C J. Foodborne Pathogens and Disease, 2011, 8(4): 465-487

10 Li S, Liu H, Deng Y, Lin L, He N. Journal of Biomedical Nanotechnology, 2013, 9(7): 1254-1260

11 LI Zhi-Yang, HE Lei, HE Nong-Yue, SHI Zhi-Yang, WANG Hua, LI Song, LIU Hong-Na, DAI Ya-Bin. Acta Chim. Sinica, 2010, 68(3): 251-256

12 HUANG Ling-Fang, DUAN Xia, CHEN Yuan, LIU Wen-Juan, WEI Hua, LAI Wei-Hua. Food Science, 2010, 31(24): 355-359