DOI: 10.3724/SP.J.1096.2013.30688
Chinese Journal of Analytical Chemistry (分析化学) 2013/41:12 PP.1851-1861
WANG Zhi-Cong
1
ZHANG Ling-Yi
1
Abstract：
An ultra-high performance liquid chromatography-diode array detection-tandem mass spectrometric (UPLC-DAD-MS/MS) method was developed for determination of phenolic compounds in chrysanthemum samples. The chromatographic separation was performed on an UPLC HSS T3 column by gradient elution with mobile phases of acetonitrile and water both containing 0.1% formic acid at a flow rate of 0.3 mL /min. The samples were extracted with aqueous methanol solution under ultrasonic condition, and the crude extracts were directly assayed without any further pretreatment. Total 10 caffeyolquinic acids (CQAs) and 22 flavonoids were positively identified in Boju, Chuju, Gongju and Hangju by comparing retention times and mass spectra of samples with standards and publications. The results showed that the amounts of phenolic compounds in four chrysanthemum samples all were agreed with the criteria of China Pharmacopoeia (2010), but there were significant differences for the content and constitute distribution of each caffeyolquinic acid and flavonoid. Except chlorogenic acid (3-CQA), 3,5-dicaffeoylquinic acid (3,5-DCQA) and luteolin-7-O-glucoside (L-7-O-Glu), other compounds with high content, such as apigenin-7-O-6″-malonylglucoside (AP-7-O-6″-MGlu), 4,5-dicaffeoylquinic acid (4,5-DCQA), 1,5-dicaffeoylquinic acid (1,5-DCQA), luteolin-7-O-6″-malonylglucoside (L-7-O-6″-MGlu) and apigenin-7-O-glucoside (AP-7-O-Glu), were also found in chrysanthemum samples. Among the four chrysanthemum samples, there were big variances for the amounts of total flavonoids, but total CQAs were found at the same level; as for total phenolic compounds in four chrysanthemum samples, Gongju showed the highest content of 66.85 mg/g, followed by Hangju, Chuju and Boju with the contents of 54.69, 44.59 and 37.10 mg/g, respectively.
ReleaseDate：2015-04-19 11:08:03
1 The Pharmacopoeia Committee of the People's Republic of China. Chinese Pharmacopoeia (Volume 1), 2010, 292
中华人民共和国药典委员会. 中国药典 (一部), 2010, 292
2 HAO Chun-Ying, ZHU Si-Si, SHI Yi-Bing, MA Ying-Li. Information on Traditional Chinese Medicine, 2012, 29(1): 24-27
郝春影, 朱思斯, 史怡冰, 马英丽. 中医药信息, 2012, 29(1): 24-27
3 WANG Yan, XIE Yuan-Yuan, SUN Jia-Hong, WANG Rui-Jie, YUAN Dan. Journal of Shenyang Pharmaceutical University, 2011, 28(2): 124-129
王 岩, 谢媛媛, 孙嘉鸿, 王瑞杰, 袁 丹. 沈阳药科大学学报, 2011, 28(2): 124-129
沈 智, 张文婷, 黄琴伟, 陈丽珏, 赵维良. 中草药, 2010, 41(2): 307-340
4 SHEN Zhi, ZHANG Wen-Ting, HUANG Qin-Wei, CHEN Li-Jue, ZHAO Wei-Liang. Chinese Traditional and Herbal Drugs, 2010, 41(2): 307-340
5 QIN Shan, WEN Xue-Sen. China Journal of Chinese Material Medica, 2011, 36(11): 1474-1477
覃 珊, 温学森. 中国中药杂志, 2011, 36(11): 1474-1477
吴德玲, 金传山, 李 琼, 俞年军, 王桐生, 张 伟. 中药材, 2011, 34(10): 1556-1558
6 WU De-Ling, JIN Chuan-Shan, LI-Qiong, YU Nian-Jun, WANG Tong-Sheng, ZHANG Wei. Journal of Chinese Medicinal Materials, 2011, 34(10): 1556-1558
7 JIA Ling-Yun, SUN Qi-Shi, HUANG Shun-Wang. Chinese Journal of Medicinal Chemistry, 2003, 13(3): 159-161
贾凌云, 孙启时, 黄顺旺. 中国药物化学杂志, 2003, 13(3): 159-161
刘 菲, 杨 洋, 谭晓杰, 毕开顺, 贾 英. 中国中药杂志, 2009, 34(16): 2067-2069
8 LIU Fei, YANG Yang, TAN Xiao-Jie, BI Kai-Shun, JIA Ying. China Journal of Chinese Material Medica, 2009, 34(16): 2067-2069
汤 毅, 高美华, 姚美村. 中药材, 2009, 32(10): 1532-1534
9 TANG Yi, GAO Mei-Hua, YAO Mei-Cun. Journal of Chinese Medicinal Materials, 2009, 32(10): 1532-1534
李瑞明, 宋伟峰, 陈 杰, 喻丽元, 梁耀荣, 张俊鹏. 今日药学, 2012, 22(9): 513-515
10 Lin L Z, Harnly J M. Food Chem., 2010, 120(1): 319-326
11 Xie Y Y, Qu J L, Wang Q L, Wang Y, Yoshikawa M, Yuan D. J. Agric. Food Chem., 2012, 60(51): 12574-12583
12 Lai J P, Lim Y H, Su J, Shen H M, Ong C N. J. Chromatogr. B, 2007, 848(2): 215-225
13 Clifford M N, Wu W G, Kirkpatrick J, Kuhnert N. J. Agric. Food Chem., 2007, 55(3): 929-936
14 LI Rui-Ming, SONG Wei-Feng, CHEN Jie, YU LI-Yuan, LIANG Yao-Rong, ZHANG Jun-Peng. Pharmacy Today, 2012, 22(9): 513-515
15 Lin L Z, Harnly J M. J. Agric. Food Chem., 2008, 56(21): 10105-10114
16 Gouveia S C, Castilho P C. Food Res. Int., 2012, 45(1): 362-368
17 Schütz K, Kammerer D, Carle R, Schieber R. J. Agric. Food Chem., 2004, 52(13): 4090-4096
18 Cuyckens F, Ma Y L, Pocsfalvi G, Claeys M. Analusis, 2000, 28(10): 888-895
19 Rijke E, Out P, Niessen W M, Ariese F, Gooijer C, Brinkman U. J. Chromatogr. A, 2006, 1112(1): 31-63
20 Ma Y L, Li Q M, Heuvel H, Claeys M. Rapid Commun. Mass spectrum., 1997, 11(12): 1357-1364
21 Cuyckens F, Claeys M. J. Mass Spectrom., 2004, 39(1): 1-15