DOI: 10.3724/SP.J.1096.2013.30667

Chinese Journal of Analytical Chemistry (分析化学) 2013/41:12 PP.1869-1874

Determination of 5 Kinds of Biogenic Amines in Bonito by Matrix Solid Phase Dispersion Extraction with Hydrophilic Interaction Chromatography-Tandem Mass Spectrometry

A method of hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS) was established for the simultaneous determination of five kinds of biogenic amines (cadaverine, histamine, phenylethylamine, tyramine and tryptamine) in bonito. The five biogenic amines were extracted by matrix solid phase dispersion (MSPD), then the extract was separated on a HILIC column with the mobile phases of acetonitrile and 50 mmol/L ammonium formate (pH 4.0 adjusted with formic acid) in a gradient elution mode. The electrospray source was operated in the positive ion mode, and monitored in the multiple reaction monitoring (MRM) mode. The linearity of five biogenic amines was good with the concentration between 0.005 to 0.200 mg/L, and the limit of quantification of the five biogenic amines was 0.1 mg/kg. The recoveries ranged from 74.5% to 116.5% with the relative standard deviations in the range of 3.8%-13.9% (n=6). The results indicated that the method was simple, rapid and accurate for the determination of five biogenic amines in bonito without the need of derivatization.

Key words:Matrix solid phase dispersion,Hydrophilic interaction chromatography-tandem mass spectrometry,Biogenic amines,Bonito

ReleaseDate:2015-04-19 11:08:07

1 HE Qing-Hua,WU Yong-Ning,YIN Yu-Long. Chinese J. Food Hygiene, 2007, 19(5): 451-454

何庆华,吴永宁,印遇龙. 中国食品卫生杂志, 2007, 19(5): 451-454

2 WANG Ying,QIU Po,HAN Bei-ZHong,YIN Li-Jun. China Brewing, 2011, 235(10): 1-5

王 颖, 邱 皤, 韩北忠, 殷丽君. 中国酿造, 2011, 235(10): 1-5

3 Mah J H, Han H K, Oh Y J, Kim M G, Hwang H J. Food Chem., 2002, 79(2): 239-243

赵中辉, 林 洪, 李振兴. 现代食品科技, 2011, 27(2): 228-231

水产品卫生标准的分析方法. 中华人民共和国国家标准GB/T 5009.45-2003

4 Dugo G, Vilasi F, Torre G L, Pellicanò T M. Food Chem., 2006, (4): 672-676

5 Onal A.Food Chem., 2007, 103(7): 1475-1486

6 Becker K, Southwick K, Reardon J, Berg R, MacCormack J N. J. A. M. A., 2001, 285(10): 1327-1330

7 Kung H F, Lee Y C, Huang Y R, Lin W F, Lin C M, Chen W C, Tsai Y H. Food Control, 2010, 21(7): 977-982

8 Lapa-Guimaráes J, Pickova J. J. Chromatogr. A, 2004, 1045(1-2): 223-232

9 Saccani G, Tanzi E, Pastore P, Cavalli S, Rey M. Food Chem., 2007, 105(4): 1652-1658

10 Paik M J, Choi Y, Kim K R. Anal. Chim. Acta, 2006, 560(1-2): 218-226

11 Antoine F R, Wei C I, Otwell W S, Sims C A, Littell R C, Hogle A D, Marshall M R. J. Agric. Food Chem., 2002, 50(17): 4754-4759

12 Hernández-Borges J, D'Orazio G, Aturki Z, Fanali S. J. Chromatogra. A, 2007, 1147(2): 192-199

13 ZHAO Zhong-Hui, LIN Hong, LI Zhen-Xing. Modern Food Science and Technology, 2011, 27(2): 228-231

14 AOAC Official Method 977.13, Histamine in Sea food Fluorometric Method

15 GB/T 5009.45-2003, Method for Analysis of Hygienic Standard of Fish and Other Aquatic Products. National Standards of the Peoples Republic of China

16 Bolygo E, Cooper P A, Jessop K M, Moffatt F. Journal of AOAC International, 2000, 83(1): 89-94

17 Calbiani F, Careri M, Elviri L, Mangia A, PistaràL, Zagnoni I. Journal of Agricultural and Food Chemistry, 2005, 53: 3779-3783