doi:

DOI: 10.3724/SP.J.1096.2013.30253

Chinese Journal of Analytical Chemistry (分析化学) 2013/41:12 PP.1919-1922

Ionic Liquid-Based Dispersive Liquid-Liquid Microextraction Coupled with Capillary Electrophoresis to Determine Drugs of Abuse in Urine


Abstract:
A method for dispersive liquid-liquid microextraction using ionic-liquid was applied for pre-concentration, separation and simultaneous detection of trace-amount ephedrine and ketamine drugs in urine samples,by coupling with high performance capillary electrophoresis. Various experimental conditions, including selection of ionic-liquid and dispersant and their concentrations, the amount of addition of NaOH and HCl in the sample, were investigated and optimized. The pretreated sample (0.5 mL) was added into 3.5 mL 0.2 g/L NaOH solution and its extraction was performed within several seconds by using 40 mL [BMIM]PF6 as extraction solvent and 350 mL acetonitrile as dispersant, then the analytes were back-extracted into 60 μL 0.1 mol/L HCl solution. Under the optimum conditions, the method shows good linearity for detection of ephedrine and ketamine in the range of 0.10-10 mg/L (r2 is 0.9968 for ephedrine and 0.9981 for ketamine). The enrichment factors for ephedrine and ketamine were achieved to be 28.8 and 16.9, respectively, and the LODs were determined to be 0.015 and 0.03 mg/L, respectively (S/N=3). The method show good repeatability with RSD less than 6.8% (n=6). The feasibility of the method was demonstrated by successful determination of ephedrine and ketamine drugs in urine samples, with spiked recoveries in the range of 79%-90% and LODs (S/N=3) of 0.21 mg/L for ephedrine and 0.39 mg/L for ketamine. The method has properties such as cheap, easy-to-operate, high enrichment, sensitive detection and little interference, and would have potential application in detection of trace-amount drugs in the biological samples.

Key words:Ionic liquid,Dispersive liquid-liquid microextraction,Capillary electrophoresis,Drug of abuse

ReleaseDate:2015-04-19 11:08:23



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