doi:

DOI: 10.3724/SP.J.1145.2010.00084

Chinese Journal of Appplied Environmental Biology (应用与环境生物学报) 2010/16:1 PP.84-90

Producing Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus in Substitutive Host Spodoptera exigua*


Abstract:
The infectivity and genetic stability of Dendrolimus punctatus Cytoplasmic polyhedrosis virus propagated in the substitutive host Spodoptera exigua were investigated. DpCPV-Se3, which was propagated in S. exigua for three passages, had identical electrophoresis pattern with the original isolate. They did not differ in polyhedra and virion morphology under the electron microscope. According the results of bioassays, DpCPV-Se3 had a similar infectivity with original isolate against the second instars of D. punctatus and the median lethal concentration (LC50) of DpCPV-Se3 (0.92×103 PIB/mL) was not significantly different from that of the original isolate (1.44×103 PIB/mL). Sequencing analysis indicated that the variations of S7 and S10 fragments between DpCPV-Se3 and DpCPV were minor. The results indicated that S. exigua might be a good substitutive host for the mass-production of DpCPV for biological control. Fig 6, Tab 2, Ref 23

Key words:Dendrolimus punctatus,cytoplasmic polyhedrosis virus,Spodoptera exigua,substitutive host

ReleaseDate:2014-07-21 15:16:33

Funds:Supported by the Special Project of Poverty Alleviation of Chinese Academy of Sciences (No. KSCX2-YW-N-011)



1 Chen CJ (陈昌洁). Integrated Management of Pine Caterpillars in China. Beijing, China (北京): China Forestry Publishing House, 1990

2 Chen CJ (陈昌洁).Wang ZX (王志贤), Liu G (刘革), Gao ZH (高志和), Tao L (陶粮), Chen JY (陈建寅), Wang ZN(王震南). Studies on introduction and utilization of the pine caterpillar Dendrolimus spectabilis CPV. For Res (林业科学研究), 1988, 1 (1): 14~24

3 Laitinen AM, Otvos IS, Levin DB. Geographic distribution of cytoplasmic polyhedrosis virus infection in Douglas-fir tussock moth larvae, Orgyia pseudotsugata, in British Columbia. J Invertebr Pathol, 1996, 67(3): 229~235

4 Zhao TH (赵同海), Chen CJ (陈昌洁), Xu J (徐静), Zhang QW (张青文). Host range and cross infection of cytoplasmic polyhedrosis viruses from Dendrolimus spp. Acta Entomol Sin (昆虫学报), 2004, 47 (1): 117~123

5 Zhao SL (赵淑玲), Liang CY (梁昌镛), Hong JJ (洪靖君), Peng HY (彭辉银). cDNA clone and sequence analysis of segments 4 of Dendrolimus punctatus cytoplasmic polyhedrosis virus. Virol Sin (中国病毒学). 2003, 18 (3): 246~250

6 Finney DJ. Probit Analysis. 3rd ed. London, UN: Cambridge University Press, 1973

7 Liu RZ (刘润忠), Xie TE (谢天恩), Peng HY (彭辉银), Chen XW (陈新文), Chen SW (陈世维). Studies on fine structure and some physico-chemical characteristics of a cytoplasmic polyhydrosis virus from Dendrolimus punctatus Wenshanensis. VirolSin (中国病毒学), 1992, 7 (1): 69~79

8 Sokal RR, Rohlf FJ. Biometry: The Principles and Practices of Statistics in Biological Research. 3rd ed. New York, USA: W. H. Freeman, 1994

9 Zhao SL, Liang CY, Hong JJ, Xu HG, Peng HY. Molecular characterization of segments 7-10 of Dendrolimus punctatus cytoplasmic polyhedrosis virus provides the complete genome. Virus Res, 2003, 94 (1): 17~23

10 Zhao SL, Liang CY, Hong JJ, Peng HY. Genomic sequence analyses of segments 1 to 6 of Dendrolimus punctatus cytoplasmic polyhedrosis virus. Arch Virol, 2003, 148 (7): 1357~1368

11 Xu HG (徐红革), Gao S (高嵩), Bai ZQ (白志强), Hong JJ (洪靖君), Ding Q (丁强), Xiao YZ (肖宇宙), Chen S (陈四), Peng HY (彭辉银). Studies on affected factors of high output of Dendrolimus punctatus cypovirus insecticide. Virol Sin (中国病毒学), 2003, 18 (4): 340~343

12 He LH (何丽华), Niu BL (牛宝龙), Weng HB (翁宏飚), Shen WF (沈卫锋), Wu ZL (吾中良), Meng ZQ (孟智启). Artificial rearing of Dendrolimus punctatus. For Pest & Dis (中国森林病虫), 2005, 24 (6): 31~34

13 Chen CJ (陈昌洁), Wang ZX (王志贤), Tao L (陶粮), Liu G (刘革). The replication of Dendrolimus punctatus CPV by Heliothis armigera. For Res (林业科学研究), 1990, 3 (3): 263~265

14 Zeng CX (曾陈湘), Wu RG (吴若光), He XX (何雪香). Screening of DpCPV`s substitutional hosts. Guangdong For Sci (广东林业科技), 1996, 12 (2): 22~27

15 Lü HS (吕鸿声). Molecular Biology of Insect Virus. Beijing, China (北京): China Agricultural Scientech Press (中国农业科技出版社), 1998

16 Zhao TH (赵同海), Chen CJ (陈昌洁). Research advance on electrophoretypes of different strains of cytoplasmic polyhedrosis viruses from Dedrolimus spp. Entomol Knowl (昆虫知识), 2004, 3 (42): 212~216

17 Li Y, Zhang J, Tan L, Chen W, Luo H, Hu Y. Phylogenetic analysis of Heliothis armigera cytoplasmic polyhedrosis virus type 14 and a series of dwarf segments found in the genome. J Gen Virol, 2007, 88: 991~997

18 Li Y, Tan L, Chen W, Zhang J, Hu Y. Identification and genome characterization of Heliothis armigera cypovirus types 5 and 14 and Heliothis assulta cypovirus type 14. J Gen Virol, 2006, 87: 387~394

19 Tan L, Zhang J, Li Y, Jiang H, Cao X, Hu Y. The complete nucleotide sequence of the type 5 Helicoverpa armigera cytoplasmic polyhedrosis virus genome. Virus Genes, 2008, 36 (3): 587~593

20 Li GH (李广宏), Pang Y (庞义), Chen QJ (陈其津), Su ZJ (苏志坚). Further studies on Spodoptera exigua artificial diet. Virol Sin (中国病毒学), 2000, 15 (Special issue): 259

21 Jiang XF (江幸福), Luo LZ (罗礼智), Hu Y (胡毅). Influece of larval diets on development, fecundity and flight capacity of the beet armyworm, Spodoptera exigua. Acta Entomol Sin (昆虫学报), 1999, 42 (3): 270~276

22 Li SC (李淑清). Effect of temperature and humidity on the development of Spodoptera exigua (Hübner). J Huazhong Agric Univ (华中农业大学学报), 2002, 21 (4): 352~355

PDF