DOI: 10.3724/SP.J.1145.2013.00090

Chinese Journal of Appplied Environmental Biology (应用与环境生物学报) 2013/19:1 PP.90-95

S144.2Optimization of Solid-state Fermentation Conditions for Antagonistic Bacillus subtilis SQR9 Producing Bio-organic Fertilizer

Bacillus subtilis SQR9 is an antagonist with a very broad antagonistic spectrum to soil borne pathogens. Production of biofertilizer containing SQR9 using agricultural waste is very important to reuse agricultural waste as sources and biocontrol soil borne plant diseases. In the present study, amino acid organic fertilizer, pig slurry compost, cattle manure compost and Chinese medicinal herb residue compost were used as ferment materials to investigate their optimal ratio and optimal fermenting conditions for SQR9 to produce bio-organic fertilizer. The optimum parameters were obtained as follows: Chinese medicinal herb residue compost as principal raw material with 10% of inoculation, 15% of wheat bran supplementation, 65% of initial moisture content, fermentation temperature 37 ℃, and once a day of turning over. Under the optimized conditions, the bacterial number of SQR9 could reach to 1.20×1011 CFU g-1. The optimum proportion of four organic composts was studied by orthogonal test. The optimum proportion obtained was that Chinese medicinal herb residue compost : cattle manure compost : pig slurry organic fertilizer : amino acid organic fertilizer was 40 : 6 : 0 : 8, in which the bacterial number of SQR9 could be as high as 1.97×1010 CFU g-1. This research therefore suggested that high quality biofertilizer could be produced using certain agricultural waste under suitable condition. Fig 5, Tab 5, Ref 33

Key words:Bacillus subtilis,solid-state fermentation,fermentation condition optimization,bio-organic fertilizer

ReleaseDate:2014-07-21 16:57:39

Funds:Fund Project for Science and Technology Achievement Transformation of Jiangsu Province (No. BA2008027) and the Ministry of Science and Technology of China (No. 2009120)