doi:

DOI: 10.3724/SP.J.1145.2012.01043

Chinese Journal of Appplied Environmental Biology (应用与环境生物学报) 2012/18:6 PP.1043-1048

Phylogenetic Diversity Analysis of Archaeal in the Pit Mud with Different Cellar Age


Abstract:
In order to investigate the clusters and succession rules of archaeal in the pit mud,the total DNAs were extracted from the pit mud with cellar age of 20,50 and 150 years,respectively.The community structure was studied by polymerase chain reaction-amplified rDNA restriction analysis(PCR-ARDRA) and sequencing of 16S rDNA.The results showed that 191 clones from the 16S rDNA library fell into 22 operational taxonomic units(OTUs) from 6 groups including Methanosarcina,Methanobacterium,Methanobacterium,Methanocorpusculum,Methanosaeta and Methanospirillum.Diversity was obvious in the predominant archaeal from the pit mud with different cellar age.The predominant archaeal from the pit mud with cellar age of 20 years were Methanosarcina and Methanosaeta,which produced methane by utilizing acetic acid.However,the predominant archaeal from the pit mud with cellar age of 50 and 150 years were Methanocorpusculum and Methanobacterium,which utilizing hydrogen.With the increasing cellar age,the Shannon-Wiener index(H) and Berger-Parker index(D) of archaeal in the pit mud showed an increasing trend,while the Pielou index(J) showed a decreasing trend.The Sorenson similarity index of the pit mud with different cellar age was the range of 0.429~0.677,and there was the highest similarity of community structure between the pit mud with cellar age of 50 and 150 years.This study revealed the relationship between archaeal phylogenetic diversity of pit mud and the aging of pit mud.

Key words:pit mud,archaeal community,16S rDNA sequence,clone library,phylogenetic,diversity

ReleaseDate:2015-04-14 17:41:03



[1] Yue YY (岳元媛), Zhang WX (张文学), Liu X (刘霞), Hu C (胡承),?Zhang SY (张宿义). Isolation and identi fi cation of facultativ e anaerobes?in the pit mud of Chinese Luzhou- fl avor liquor. Microbiol Chin (微生物学通报),2007,34(2):251~255

[2]Wu YY (吴衍庸), Lu SH (卢世珩), Liu GY (刘光烨). A study of?enhancing the quality of Luzhou type Qujiu by using simulataneous?fermentation of caproic acid bacteria and Methane bacteria. Food &?Ferment Ind (食品与发酵工业),1990,6:2~6

[3]Huang ZG (黄治国), Zhen P (甄攀), Luo HB (罗惠波). Research on?archaea community in Luzhou- fl avor liquor pits. J Southwest University?Nat Sci Ed (西南大学学报自然科学版),2010,32(12):91~96

[4]Shi S (施思), Wang HY (王海英), Zhang WX (张文学), Deng Y (邓宇), Lai DY (赖登燡), Fan A (范鏖). Analysis of microbial communities?characteristics in different pit mud of Luzhou-flavor liquor. LiquorMaking Sci & Technol (酿酒科技),2011(5):38~41

[5]Ulrich A, Becker R. Soil parent material is a key determinant of the?bacterial community structure in arable soils. FEMS Microbiol Ecol.2006,56(3):430~443

[6]Lu LQ (卢莉琼), Xu YT (徐亚同), Liang J (梁俊). Perspective of?molecular biological techniques applied in microbiological diversity and?microbiological ecology. Chin J Appl Environ Biol (应用与环境生物学报),2004,10(6):826~830

[7]Bai YH (柏耀辉), Sun QH (孙庆华), Wen DH (温东辉), Tang XY (唐孝炎). Application of molecular ecological techniques on the study of?microbial nitri fi cation and denitri fi cation. Acta Sci Nat Univ Pekinensis?(北京大学学报自然科学版),2011,47(2):378~384

[8]Zhang WX (张文学), Qiao ZW (乔宗伟), Hu C (胡承), Wang ZY (王忠彦). Analysis of bacterial community in fermented grains during?the production of Chinese strong aromatic spirits by PCR technique. J?Sichuan Univ Eng Sci Ed (四川大学学报工程科学版),2005,37(5):82~87

[9]Shi S (施思), Hu C (胡承), Zhang WX (张文学). Preliminary study on?an actinomycete producing brown-pigment and its 16SrDNA sequence?analysis. Sci & Technol Food Ind (食品工业科技),2010,31(10):239~240

[10]Quiroz HC, Brue S, Yashiro E, Sun C, Yavitt J, Zinder S. Vertical?pro fi les of methanogenesis and methanogens in two contrasting acidic?peatlands in central New York State, USA. Environ Microbiol,2006,8(8):1428~1440

[11]Xia BC (夏北成), Zhou J, Tiedje JM. Structures of bacteria cloning?communities in the soil environment and their ecological characteristics.?Acta Ecol Sin (生态学报),2001,21(4):574~578

[12]Good IJ. The population frequencies of species and the estimation of?population parameters. Biometrika,1953,40:237~264

[13]Drancourt M, Bollet C, Carlioz A, Martelin R, Gayral JP, Raoult D.16S?ribosomal DNA sequence analysis of a large collection of environmental?and clinical unidenti fi able bacterial isolates. Clin Microbiol,2000,38:3623~3630

[14]Dianou D, Miyaki T, Asakawa S, Morii H, Nagaoka K, Oyaizuand?H, Matsumoto S. Methanoculleus chikugoensis sp. nov., a novel?methanogenic archaeon isolated from paddy field soil in Japan, and?DNA-DNA hybridization among Methanoculleus species. Int J Sys Evol?Microbiol,2001,51:1663~1669

[15]Schmidt JE, Ahring BK. Immobilization patterns and dynamics of?acetate-utilizing methanogens immobilized in sterile granular sludge in?up fl ow anaerobic sludge blanket reactors. Appl Environ Microbiol,1999,

[16]Wei CH (卫春会), Zhen P (甄攀), Luo HB (罗惠波), Yang J (杨洁).?Study on pit mud lixivium of different cellar age by gas chromatography?mass spectrometry. Sci & Technol Food Ind (食品工业科技),2011,32(2):95~97

[17]Wang LP (王俪鲆), Zhang L (张良), Liu LY (刘来雁), Zhang SY (张宿义), Xu DF (许德富), Liu GY (刘光烨). Comparative study of two?Methanobacterium strains in the ancient fermentation pits of Luzhou.?Chin J Appl Environ Biol (应用与环境生物学报),2010,16(6):840~844

[18]Fernández N, Díaz EE, Amils R, Sanz JL. Analysis of microbial?community during biofilm development in an anaerobic wastewater?treatment reactor. Microbial Ecol,2008,56(1):121~132

[19]Gao RF (高瑞芳), Yuan XF (袁旭峰), Wang XF (王小芬), Zhu?WB (朱万斌), Cheng X (程序), Cui ZJ (崔宗均). Archaeal diversity?and metabolic function in anaerobic digest process treating high?concentration ethanol wastewater. Microbiol Chin (微生物学通报),2011,38(4):468~473

[20]Maestrojuan GM, Boone DR. Characterization of Methanosarcina?barkeri MST and 227, Methanosarcina mazei S-6T, and Methanosarcina?vacuolata Z-761T. Int Journal Sys Bacteriol,1991,41(2):267~274

[21]Fu L (傅霖), Xin MX (辛明秀). Ecological diversity and industrial?application of methanogens. Chin J Appl Environ Biol (应用与环境生物学报),2009,15(4):574~578

[22]Zhang W (张薇), Hu YG (胡跃高), Huang GH (黄国和), Gao HW (高洪文). Soil microbial diversity of arti fi cial peashrub plantation on North?Loess Plateau of China. Acta Microbiol Sin (微生物学报),2007,47(5):751~756

[65](3):1050~1054